Quantification of the Antiviral Effect of Interferon by Immunoassay of Vesicular Stomatitis Virus Proteins

Fabrication and photophysical study of photofunctional nanoporous alumina membrane (PNAM) were performed, and its application of photodynamic antimicrobial chemotherapy (PACT) was investigated. Nanoporous alumina membrane (NAM) was fabricated by two-step aluminium anodic oxidation process. Surface of the fabricated NAM was modified with organo-silane agent to induce covalent bonding between NAM and a photosensitizer (PtCP: [5,10,15-triphenyl-20-(4-methoxycarbonylphenyl)-porphyrin] platinum). PtCP was covalently bonded to the surface of the modified NAM by nucleophilic acyl substitution reaction process. The morphology and the photophysical properties of the fabricated PNAM were confirmed with field emission scanning electron microscope (FE-SEM), steady-state spectroscopies, and nanosecond laser-induced time-resolved spectroscopy. For the efficacy study of PNAM in PACT, an enveloped animal virus, vesicular stomatitis virus (VSV), was utilized as a target organism. Antiviral effect of the PNAM-PACT was measured by the extent of suppression of plaque-forming units (PFU) after the light irradiation. In the cultures inoculated with PACT-treated VSV, the suppression of PFU was prominent, which demonstrates that PNAM is a potential bio clean-up tool.

Download Full-text

In situ cross-linking of vesicular stomatitis virus proteins with reversible agents

Virology ◽

10.1016/0042-6822(78)90284-2 ◽

1978 ◽

Vol 88 (2) ◽

pp. 263-280 ◽

Cited By ~ 14

Author(s):

John A. Mudd ◽

Richard E. Swanson

Keyword(s):

Vesicular Stomatitis Virus ◽

Cross Linking ◽

Vesicular Stomatitis ◽

Virus Proteins

Download Full-text

Antiviral effect of short hyperthermic treatment at specific stages of vesicular stomatitis virus replication cycle

Journal of General Virology ◽

10.1099/0022-1317-74-8-1685 ◽

1993 ◽

Vol 74 (8) ◽

pp. 1685-1690 ◽

Cited By ~ 17

Author(s):

A. De Marco ◽

M. G. Santoro

Keyword(s):

Vesicular Stomatitis Virus ◽

Virus Replication ◽

Antiviral Effect ◽

Replication Cycle ◽

Hyperthermic Treatment ◽

Vesicular Stomatitis ◽

Virus Replication Cycle

Download Full-text

Cell-membrane phospholipase C is involved in inducing the antiviral effect of interferon

Bioscience Reports ◽

10.1007/bf01119795 ◽

1989 ◽

Vol 9 (5) ◽

pp. 531-539 ◽

Cited By ~ 2

Author(s):

L. M. Popescu ◽

C. Cernescu ◽

I. I. Moraru ◽

St. N. Constantinescu ◽

F. Baltã ◽

...

Keyword(s):

Phospholipase C ◽

Vesicular Stomatitis Virus ◽

Second Messengers ◽

Calcium Ionophore ◽

Antiviral Effect ◽

Vero Cells ◽

Calcium Ionophore A23187 ◽

Ionophore A23187 ◽

Antibody Molecule ◽

Vesicular Stomatitis

A monospecific inhibitory antibody directed to phospholipase C (phosphoinositidase C) blocked the antiviral effect of human interferons alpha and beta when tested on human quiescent fibroblasts challenged with the vesicular stomatitis virus. This action was due to specific inhibition of polyphosphoinositide hydrolysis because (a) the F(ab′)2 fragment of the antibody molecule was also inhibitory; (b) excess antibodies directed to phospholipase A2 and to a phosphatidylcholine-preferring phospholipase C did not have any inhibitory effect, and (c) the combination of 12-O-tetradecanoylphorbol-acetate and calcium ionophore A23187 had an interferon-like antiviral effect which was not influenced by the inhibitory anti-phospholipase C antibodies. To avoid an interferon-like effect due to induction of interferon by second messengers, Vero cells, which lack interferon biosynthesis, were also used. Liposomes containing inositol 1,4,5-triphosphate and 1-oleoyl-2-acetyl-rac-glycerol protected Vero cells against the infection with the vesicular stomatitis virus. These results taken together show that phosphoinositide-derived second messengers are involved in triggering the antiviral effect of interferons alpha and beta.

Download Full-text