scholarly journals Fabrication of Photofunctional Nanoporous Membrane and Its Photoinactivation Effect of Vesicular Stomatitis Virus

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Kang-Kyun Wang ◽  
Bong-Jin Kim ◽  
Si-Hwan Ko ◽  
Dong Hoon Choi ◽  
Yong-Rok Kim
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Photophysical Properties ◽  
Antiviral Effect ◽  
Nanosecond Laser ◽  
Alumina Membrane ◽  
Time Resolved ◽  
Nanoporous Alumina ◽  
Animal Virus ◽  
Covalently Bonded ◽  
Vesicular Stomatitis

Fabrication and photophysical study of photofunctional nanoporous alumina membrane (PNAM) were performed, and its application of photodynamic antimicrobial chemotherapy (PACT) was investigated. Nanoporous alumina membrane (NAM) was fabricated by two-step aluminium anodic oxidation process. Surface of the fabricated NAM was modified with organo-silane agent to induce covalent bonding between NAM and a photosensitizer (PtCP: [5,10,15-triphenyl-20-(4-methoxycarbonylphenyl)-porphyrin] platinum). PtCP was covalently bonded to the surface of the modified NAM by nucleophilic acyl substitution reaction process. The morphology and the photophysical properties of the fabricated PNAM were confirmed with field emission scanning electron microscope (FE-SEM), steady-state spectroscopies, and nanosecond laser-induced time-resolved spectroscopy. For the efficacy study of PNAM in PACT, an enveloped animal virus, vesicular stomatitis virus (VSV), was utilized as a target organism. Antiviral effect of the PNAM-PACT was measured by the extent of suppression of plaque-forming units (PFU) after the light irradiation. In the cultures inoculated with PACT-treated VSV, the suppression of PFU was prominent, which demonstrates that PNAM is a potential bio clean-up tool.

Cell-membrane phospholipase C is involved in inducing the antiviral effect of interferon

1989 ◽  
Vol 9 (5) ◽  
pp. 531-539 ◽  
Author(s):  
L. M. Popescu ◽  
C. Cernescu ◽  
I. I. Moraru ◽  
St. N. Constantinescu ◽  
F. Baltã ◽  
...  
Keyword(s):  
Phospholipase C ◽  
Vesicular Stomatitis Virus ◽  
Second Messengers ◽  
Calcium Ionophore ◽  
Antiviral Effect ◽  
Vero Cells ◽  
Calcium Ionophore A23187 ◽  
Ionophore A23187 ◽  
Antibody Molecule ◽  
Vesicular Stomatitis

A monospecific inhibitory antibody directed to phospholipase C (phosphoinositidase C) blocked the antiviral effect of human interferons alpha and beta when tested on human quiescent fibroblasts challenged with the vesicular stomatitis virus. This action was due to specific inhibition of polyphosphoinositide hydrolysis because (a) the F(ab′)2 fragment of the antibody molecule was also inhibitory; (b) excess antibodies directed to phospholipase A2 and to a phosphatidylcholine-preferring phospholipase C did not have any inhibitory effect, and (c) the combination of 12-O-tetradecanoylphorbol-acetate and calcium ionophore A23187 had an interferon-like antiviral effect which was not influenced by the inhibitory anti-phospholipase C antibodies. To avoid an interferon-like effect due to induction of interferon by second messengers, Vero cells, which lack interferon biosynthesis, were also used. Liposomes containing inositol 1,4,5-triphosphate and 1-oleoyl-2-acetyl-rac-glycerol protected Vero cells against the infection with the vesicular stomatitis virus. These results taken together show that phosphoinositide-derived second messengers are involved in triggering the antiviral effect of interferons alpha and beta.

scholarly journals Study of Glutoxim antiviral activity in the fibroblast cell line infected with vesicular stomatitis virus

2018 ◽  
Vol 2018 (6) ◽  
pp. 25-29 ◽  
Author(s):  
Сергей Ожерелков ◽  
Sergey Ozherelkov ◽  
Татьяна Кожевникова ◽  
Tat'yana Kozhevnikova ◽  
Александр Санин ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Cell Lines ◽  
Vesicular Stomatitis Virus ◽  
Fibroblast Cell ◽  
Antiviral Effect ◽  
Low Doses ◽  
Vesicular Stomatitis ◽  
Cytopathic Action ◽  
Fibroblast Cell Lines

New strategy for the treatment of animal infectious diseases is based upon the modulation of the host immune response in order to enhance the clearance of infectious agents and reduce the damaging effects of inflammation in the tissues. The modern approach to the use of immunomodulators (IMD) in veterinary practice consists in the usage of such drugs, which are not only immunomodulating, but also have antiviral, antioxidant, anti-inflammatory, hemostimulating and/or other important properties. The aim of the study was to identify possible antiviral activity of known IMD Glutoxim (GLT) during infection of diploid fibroblast cell lines M-8 and M-22 with vesicular stomatitis virus (VSV). Materials and methods: VSV, strain Indiana, was used. Antiviral activity of GLT investigated: 1) at doses recommended for experiments in vitro: 1, 4 and 8 µg/ml; 2) at low doses: 0,1; 0,25 and 0,5 µg/ml. GLT was added to the cell monolayer according to preventive (for 24 hours prior to VSV infection of cells) and treatment (unanimous with VSV infection) protocols. The antiviral activity of GLT was assessed by the following criteria: ability of the drug to prevent the development of virus cytopathic action, to inhibit the reproduction of VSV, and by expessing virucidal action. Results: GLT in doses recommended for in vitro experiments (1, 4, 8 µg/ml) did not delay the development of a specific virus-induced cytopathic action. The VSV titers in infected cells in the presence of GLT did not differ from those in the control cell lines infected with VSV without the addition of GLT. The latter had no virucidal effect against the VSV. Inoculation of GLT into the cell culture at low doses of 0.1, 0.25 and 0.5 mg/ml led to a significant (more than 100-fold) inhibition of VSV replication 24 hours after infection of cells. At later stages, 40 and 48 hours following infection, the antiviral effect of GLT was not detected. Thus, we established that GLT possesses antiviral effect in vitro, which is manifested 24 hours following infection of diploid fibroblast cell lines with VSV.

626: Initial Experience with Vesicular Stomatitis Virus as a Treatment for Bladder Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 202-202
Author(s):  
Sherwin Zargaroff ◽  
Yuancheng Wang ◽  
Xiayong Zheng ◽  
Jian Pu ◽  
Savio L. Woo ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Vesicular Stomatitis Virus ◽  
Initial Experience ◽  
Vesicular Stomatitis

Inhibition of Various Steps in the Replication Cycle of Vesicular Stomatitis Virus Contributes to Its Photoinactivation by AlPcS4 or Pc4 and Red Light

1999 ◽  
Vol 69 (3) ◽  
pp. 353 ◽  
Author(s):  
Anne C. E. Moor ◽  
Angeline E. Wagenaars-van Gompel ◽  
Ralph C. A. Hermanns ◽  
Jannes van der Meulen ◽  
Jolanda Smit ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Red Light ◽  
Replication Cycle ◽  
Vesicular Stomatitis
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