RNA Polymerase Binding Sites on the Broad Host Range Plasmid RP4

Microbiology ◽  
1981 ◽  
Vol 125 (1) ◽  
pp. 189-193
Author(s):  
H.-J. BURKAR ◽  
W. WOHLLEBEN
Keyword(s):  
Rna Polymerase ◽  
Host Range ◽  
Binding Sites ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Polymerase Binding

Broad Host-Range Plasmid R1162: Replication, Incompatibility, and Copy-Number Control

1985 ◽  
pp. 173-188 ◽  
Author(s):  
Richard J. Meyer ◽  
Lung-Shen Lin ◽  
Kyunghoon Kim ◽  
Michael A. Brasch
Keyword(s):  
Host Range ◽  
Copy Number ◽  
Broad Host Range ◽  
Copy Number Control ◽  
Broad Host Range Plasmid

scholarly journals Promoters of the broad host range plasmid RK2: analysis of transcription (initiation) in five species of gram-negative bacteria.

Genetics ◽  
1992 ◽  
Vol 130 (1) ◽  
pp. 27-36 ◽  
Author(s):  
A Greener ◽  
S M Lehman ◽  
D R Helinski
Keyword(s):  
Host Range ◽  
Transcription Initiation ◽  
Promoter Activity ◽  
Firefly Luciferase ◽  
Broad Host Range ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Broad Host Range Plasmid ◽  
Transcriptional Start Sites ◽  
Plasmid Rk2

Abstract A broad host range cloning vector was constructed, suitable for monitoring promoter activity in diverse Gram-negative bacteria. This vector, derived from plasmid RSF1010, utilized the firefly luciferase gene as the reporter, since the assay for its bioluminescent product is sensitive, and measurements can be made without background from the host. Twelve DNA fragments with promoter activity were obtained from broad host range plasmid RK2 and inserted into the RSF1010 derived vector. The relative luciferase activities were determined for these fragments in five species of Gram-negative bacteria. In addition, four promoters were analyzed by primer extension to locate transcriptional start sites in each host. The results show that several of the promoters vary substantially in relative strengths or utilize different transcriptional start sites in different bacteria. Other promoters exhibited similar activities and identical start sites in the five hosts examined.

scholarly journals A broad host range plasmid vector that does not encode replication proteins

2002 ◽  
Vol 211 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Eza Kalyaeva ◽  
Irina Bass ◽  
Gennady Kholodii ◽  
Vadim Nikiforov
Keyword(s):  
Host Range ◽  
Plasmid Vector ◽  
Broad Host Range ◽  
Replication Proteins ◽  
Broad Host Range Plasmid
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