Properties of Monoclonal Antibodies to the Genus-specific Antigen of Chlamydia and Their Use for Antigen Detection by Reverse Passive Haemagglutination

SUMMARYHuman lymphatic filariasis (LF) is a major cause of disability globally. The success of global elimination programmes for LF depends upon effectiveness of tools for diagnosis and treatment. In this study on stage-specific antigen detection in brugian filariasis, L3, adult worm (AW) and microfilarial antigenaemia were detected in around 90–95% of microfilariae carriers (MF group), 50–70% of adenolymphangitis (ADL) patients, 10–25% of chronic pathology (CP) patients and 10–15% of endemic normal (EN) controls. The sensitivity of the circulating filarial antigen (CFA) detection in serum samples from MF group was up to 95%. In sera from ADL patients, unexpectedly, less antigen reactivity was observed. In CP group all the CFA positive individuals were from CP grade I and II only and none from grade III or IV, suggesting that with chronicity the AWs lose fecundity and start to disintegrate and die. Amongst EN subject, 10–15% had CFA indicating that few of them harbour filarial AWs, thus they might not be truly immune as has been conventionally believed. The specificity for antigen detection was 100% when tested with sera from various other protozoan and non-filarial helminthic infections.

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Monoclonal Antibodies to a Tumor Specific Antigen on Rat Mammary Carcinoma Sp4 and Their Use in Drug Delivery Systems

Hybridomas and Cellular Immortality ◽

10.1007/978-1-4615-9352-2_12 ◽

1983 ◽

pp. 185-200

Author(s):

R. W. Baldwin ◽

M. J. Embleton ◽

G. R. Flannery ◽

B. Gunn ◽

J. A. Jones ◽

...

Keyword(s):

Drug Delivery ◽

Monoclonal Antibodies ◽

Mammary Carcinoma ◽

Drug Delivery Systems ◽

Specific Antigen ◽

Delivery Systems ◽

Tumor Specific Antigen

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Analysis of human bone marrow with monoclonal antibodies.

Journal of Histochemistry & Cytochemistry ◽

10.1177/33.12.2415573 ◽

1985 ◽

Vol 33 (12) ◽

pp. 1183-1189 ◽

Cited By ~ 2

Author(s):

P J Thurlow ◽

L Kerrigan ◽

R A Harris ◽

I F McKenzie

Keyword(s):

Bone Marrow ◽

Monoclonal Antibodies ◽

Specific Antigen ◽

Cell Types ◽

Diagnostic Value ◽

Immunofluorescence Assay ◽

Simple Method ◽

Antigenic Phenotype ◽

Beta 2 Microglobulin ◽

Cellular Phenotypes

In order to study the antigenic phenotype of different hemopoietic cells, we used a series of monoclonal antibodies to investigate normal bone marrow in a standard immunofluorescence assay. The antibodies detected the following antigens: HLA-ABC, beta 2-microglobulin (beta 2m), HLA-DR (Ia), a lymphocyte subset and specific antigen (T and B) HuLy-m2, m3, T lymphocyte antigen (HuLy-m1), lymphocyte T200 antigen (HuLy-m4), a viral-associated antigen (HuLy-m5), and platelet-specific glycoproteins IIb-IIIa (HuPl-m1). The following results were obtained: (a) normoblasts were weakly HLA-ABC+, beta 2m+ and Ia-; all other lymphocyte and platelet antigens were not detected. (b) Myeloid cells at all stages of differentiation (promyelocytes, myelocytes, metamyelocytes, and neutrophils) were HLA-ABC+; beta 2m+; HuLy-m1-, m2-, m3+/- (20%), m4+, m5+/- (20%); HuPl-m1-; in addition, promyelocytes and myelocytes were Ia+ but neutrophils and metamyelocytes were Ia-. (c) Lymphocytes were HLA-ABC+, beta 2m+, Ia+/- (20-30%), HuLy-m1+/- (40-50%), m2+/- (60-70%), m3+, m4+, m5+; Pl-m1-. (d) Platelets and megakaryocytes were HLA-ABC+; beta 2m+; Ia-; HuLy-m1+-, m2-, m3-, m4-, m5-, HuPl-m1+, and the putative "megakaryocyte precursors" were HuPl-m1+, Ia-, HuLy-m1-. The different cell types in bone marrow could readily be distinguished, particularly cells of the myeloid series (Ia and HuLy-m4, m5), lymphocytes (Ia and HuLy-m1, m2, m3), and platelets and their precursor cells (HuPl-m1). This simple method of defining cellular phenotypes in bone marrow has demonstrated the practicality of using monoclonal antibodies to identify marrow cells and should be of diagnostic value.

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