Electron Microscope Observations on the Structure of Fimbriae, with Particular Reference to Klebsiella Strains, by the use of the Negative Staining Technique

After intoxication of rabbits with certain substances such as convulsant agents (3-acetylpyridine), centrally acting drugs (reserpine), or toxic metal compounds (tetraethyl lead) a significant observation by phase microscope is the loss of contrast of the hippocampal mossy fiber layer. It has been suggested that this alteration, as well as changes seen with the electron microscope in the hippocampal mossy fiber boutons, may be related to a loss of neurotransmitters. The purpose of these experiments was to apply the OsO4-zinc-iodide staining technique to the study of these structural changes since it has been suggested that OsO4-zinc-iodide stain reacts with neurotransmitters (acetylcholine, catecholamines).Domestic New Zealand rabbits (2.5 to 3 kg) were used. Hippocampal tissue was removed from normal and experimental animals treated with 3-acetylpyridine (antimetabolite of nicotinamide), reserpine (anti- hypertensive/tranquilizer), or iproniazid (antidepressant/monamine oxidase inhibitor). After fixation in glutaraldehyde hippocampal tissue was treated with OsO4-zinc-iodide stain and further processed for phase and electron microscope studies.

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Early developments in the negative staining technique for electron microscopy

Micron and Microscopica Acta ◽

10.1016/0739-6260(91)90051-z ◽

1991 ◽

Vol 22 (4) ◽

pp. 321-326 ◽

Cited By ~ 5

Author(s):

R.W. Horne

Keyword(s):

Electron Microscopy ◽

Staining Technique ◽

Negative Staining

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A negative staining—carbon film technique for studying viruses in the electron microscope

Journal of Ultrastructure Research ◽

10.1016/s0022-5320(74)90015-x ◽

1974 ◽

Vol 47 (3) ◽

pp. 361-383 ◽

Cited By ~ 110

Author(s):

R.W. Horne ◽

Ivonne Pasquali Ronchetti

Keyword(s):

Electron Microscope ◽

Carbon Film ◽

Negative Staining

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The fine structure and the protein composition of γ phage of Bacillus anthracis

Canadian Journal of Microbiology ◽

10.1139/m75-275 ◽

1975 ◽

Vol 21 (11) ◽

pp. 1889-1892 ◽

Cited By ~ 18

Author(s):

Takashi Watanabe ◽

Akinori Morimoto ◽

Toshiro Shiomi

Keyword(s):

Electron Microscopy ◽

Fine Structure ◽

Bacillus Anthracis ◽

Protein Composition ◽

Staining Technique ◽

Negative Staining ◽

Polyacrylamide Gels ◽

Long Tail

The fine structure of γ phage of Bacillus anthracis was studied by electron microscopy with a negative-staining technique. The phage has a hexagonal head and a long tail without a sheath. By electrophoresis on polyacrylamide gels, the proteins of the phage particles are separate into 10 polypeptides with moleclar weights ranging from 140 000 to 12 000.

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