The adsorption staining technique applied to isolated premessenger ribonucleoprotein particles: A comparison with conventional techniques using electron microscope tomography

1993 ◽  
Vol 170 (2) ◽  
pp. 173-182 ◽  
Author(s):  
A. LÖNNROTH ◽  
U. SKOGLUND ◽  
K. T. TOKUYASU ◽  
B. DANEHOLT
Keyword(s):  
Electron Microscope ◽  
Staining Technique ◽  
Ribonucleoprotein Particles

The Reaction of Brain Structures with OsO4-Zinc-Iodide Stain

1971 ◽  
Vol 29 ◽  
pp. 272-273
Author(s):  
Werner J. Niklowitz
Keyword(s):  
Electron Microscope ◽  
Structural Changes ◽  
Mossy Fiber ◽  
Toxic Metal ◽  
Staining Technique ◽  
Brain Structures ◽  
Oxidase Inhibitor ◽  
Fiber Layer ◽  
Hippocampal Tissue ◽  
Zinc Iodide

After intoxication of rabbits with certain substances such as convulsant agents (3-acetylpyridine), centrally acting drugs (reserpine), or toxic metal compounds (tetraethyl lead) a significant observation by phase microscope is the loss of contrast of the hippocampal mossy fiber layer. It has been suggested that this alteration, as well as changes seen with the electron microscope in the hippocampal mossy fiber boutons, may be related to a loss of neurotransmitters. The purpose of these experiments was to apply the OsO4-zinc-iodide staining technique to the study of these structural changes since it has been suggested that OsO4-zinc-iodide stain reacts with neurotransmitters (acetylcholine, catecholamines).Domestic New Zealand rabbits (2.5 to 3 kg) were used. Hippocampal tissue was removed from normal and experimental animals treated with 3-acetylpyridine (antimetabolite of nicotinamide), reserpine (anti- hypertensive/tranquilizer), or iproniazid (antidepressant/monamine oxidase inhibitor). After fixation in glutaraldehyde hippocampal tissue was treated with OsO4-zinc-iodide stain and further processed for phase and electron microscope studies.

Ultrastructure of polytene chromosomes of Drosophila isolated by microdissection

1980 ◽  
Vol 45 (1) ◽  
pp. 15-30
Author(s):  
M.R. Mott ◽  
E.J. Burnett ◽  
R.J. Hill
Keyword(s):  
Electron Microscope ◽  
Polytene Chromosomes ◽  
Ultrastructural Level ◽  
Chromosomal Proteins ◽  
Linear Arrays ◽  
Ribonucleoprotein Particles ◽  
Acid Protein

Drosophila polytene chromosomes prepared by a new micromanipulative procedure, which avoids acid squashing, have been examined at the ultrastructural level in the electron microscope. Puffs at 2B, 68C, 74EF, 75B and 85EF, have been examined in some detail, along with the chromocentre and various interbands. The ultrastructure of these chromosomes, which have never been exposed to acid protein denaturants, compares favourably with that of classical acid-fixed specimens. Ribonucleoprotein particles in puffs are seen to be organized in linear arrays and evidence is adduced for looped transcription units. Particles with characteristic sizes and morphologies are observed near the chromocentre, in puffs and in interbands. In interbands RNP particles and ‘superbead’-like chromatin particles may be distinguished. Drosophila polytene chromosomes isolated by micro-manipulation should prove useful for the localization of native chromosomal proteins at an ultrastructural level.

Ultrastructural Characterization of Liposomes Using Transmission Electron Microscope

2008 ◽  
Vol 55-57 ◽  
pp. 709-711 ◽  
Author(s):  
Prukswan Chetanachan ◽  
P. Akarachalanon ◽  
D. Worawirunwong ◽  
Pisutti Dararutana ◽  
A. Bangtrakulnonth ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Polymeric Nanoparticles ◽  
Staining Technique ◽  
Bilayer Membrane ◽  
Multilamellar Vesicles ◽  
Transmission Electron ◽  
Ultrastructural Characterization ◽  
Film Method

A liposome is a spherical vesicle composed of phospholipids and cholesterol bilayer membrane and contains a core of aqueous solution. Liposomes are polymeric nanoparticles used for drug delivery due to their unique properties. It can carry both hydrophobic and hydrophilic molecules. In this study, we showed the benefit of using transmission electron microscope (TEM) with negative staining technique to investigate the morphology of liposomes produced by thin film method. At the same magnification of micrograph results, we could see the multilamellar vesicles of liposomes in various figures and different sizes.

scholarly journals THE ULTRASTRUCTURE OF HUMAN THYROGLOBULIN

1968 ◽  
Vol 128 (5) ◽  
pp. 1129-1136 ◽  
Author(s):  
B. Bloth ◽  
R. Bergquist
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Carbon Film ◽  
Gel Filtration ◽  
Staining Technique ◽  
Single Step ◽  
Inhibiting Activity ◽  
Sedimentation Constant ◽  
Maximal Diameter ◽  
Step Procedure

Thyroglobulin molecules purified in a single step procedure by gel filtration were studied in the electron microscope using the negative staining technique. The molecule had the shape of a flexible helix with two turns. Its length was about 220 A and the maximal diameter of the coiled part of the molecule was estimated to be 110 A. The pitch varied between 40 and 50 A. Thyroglobulin molecules dried in sodium tungstosilicate on a carbon film as for electron microscopy retained their hemagglutination-inhibiting activity and 19S sedimentation constant when redissolved in physiological buffer.

Enzymatisch isolierte Cellulose-Fibrillen der Valonia-Zellwand

1968 ◽  
Vol 23 (2) ◽  
pp. 272-274 ◽  
Author(s):  
Werner W. Franke ◽  
Heinz Falk
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Cross Section ◽  
Staining Technique ◽  
Cellulose Microfibrils ◽  
Prolonged Treatment ◽  
Cellulose Chain ◽  
Chain Molecules ◽  
Elementary Fibril ◽  
20 Nm

Cellulose microfibrils from the cell wall of the green alga Valonia macrophysa were isolated by enzymatical digestion of the wall matrix and examined in the electron microscope using the negative staining technique. The smallest fibrils obtained after prolonged treatment were found to be flat ribbons with a width about 10—20 nm and a height of 3 to 4 nm. This result is discussed in relation to Frey- Wyssling's concept of an “elementary fibril“ with a cross-section of 3.5 × 3.5 nm. Some alternation of unstained areas along the fibrils was observed. This was interpreted as artificially induced rather than relating to the structure and the arrangement of the cellulose chain molecules.

The arrangement of myosin on the surface of paramyosin filaments in the white adductor muscle of Crassostrea angulata

1974 ◽  
Vol 186 (1082) ◽  
pp. 53-66 ◽  
Keyword(s):  
Electron Microscope ◽  
Staining Technique ◽  
Adductor Muscle ◽  
Uranyl Acetate ◽  
Acetate Solution ◽  
Left Handed ◽  
Thick Filaments ◽  
Crassostrea Angulata ◽  
Regular Arrangement ◽  
Made In

Observations of the surface of intact thick filaments from the oyster Crassostrea angulata have been made in the electron microscope. The surface structure has been revealed by metal shadowing and by a negative staining technique in which aqueous uranyl acetate solution is applied to filaments which have been rendered impervious to this solution. Both methods reveal a regular arrangement of round objects on the filament, interpreted as groups of myosin heads. The arrangement is that of the Bear-Selby net, probably with two or three myosin molecules per node. The possibility is discussed that the helical strands which give rise to the Bear-Selby net may occur in right- and left-handed forms in different filaments.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF CANINE CARDIAC MYOSIN AND SOME OF ITS AGGREGATES

1966 ◽  
Vol 28 (2) ◽  
pp. 375-389 ◽  
Author(s):  
John A. Carney ◽  
Arnold L. Brown
Keyword(s):  
Electron Microscope ◽  
Statistical Analysis ◽  
Electron Microscope Study ◽  
Central Zone ◽  
Staining Technique ◽  
Myosin Molecule ◽  
Cardiac Myosin ◽  
Replica Technique ◽  
Skeletal Myosin ◽  
The Mean

The morphology of the canine cardiac myosin molecule has been investigated in the electron microscope with Hall's mica-replica technique. The molecule is an elongated rod (shaft) of nonuniform diameter with a globular expansion (head) on one end. Statistical analysis of the lengths of 1908 molecules showed that the mean length was 1610 ± 250 A; the mean length of the head was 210 ± 20 A; and the diameter of the head and that of the shaft were 35 to 40 and 15 to 20 A, respectively. About one-third of the molecules had single or multiple, fairly sharp, angulations along their shafts. Rarely, some details of the substructure of the molecule have been observed. Large, spindle-shaped aggregates, measuring 0.5 to 1 µ in length and 50 to 100 A in diameter, were produced by dilution of the myosin solutions. These aggregates were readily visualized in the electron microscope by means of Huxley's negative-staining technique. Projections often were visible along the length of the aggregates except at a central zone where they were frequently absent. The aggregates resembled the thick myofilaments of the myocardium and appeared similar to those produced by Huxley from skeletal myosin solutions.

Electron Cytochemical Study of Carbon Dioxide Laser Effect on Rhesus Monkey Cornea

1974 ◽  
Vol 32 ◽  
pp. 224-225
Author(s):  
K. C. Tsou ◽  
J. Morris ◽  
P. Shawaluk ◽  
B. Stuck ◽  
E. Beatrice
Keyword(s):  
Carbon Dioxide ◽  
Electron Microscope ◽  
Rhesus Monkey ◽  
Carbon Dioxide Laser ◽  
Cytochemical Study ◽  
Laser Effect

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

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