Early developments in the negative staining technique for electron microscopy

1991 ◽  
Vol 22 (4) ◽  
pp. 321-326 ◽  
Author(s):  
R.W. Horne
Keyword(s):  
Electron Microscopy ◽  
Staining Technique ◽  
Negative Staining

The fine structure and the protein composition of γ phage of Bacillus anthracis

1975 ◽  
Vol 21 (11) ◽  
pp. 1889-1892 ◽  
Author(s):  
Takashi Watanabe ◽  
Akinori Morimoto ◽  
Toshiro Shiomi
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Bacillus Anthracis ◽  
Protein Composition ◽  
Staining Technique ◽  
Negative Staining ◽  
Polyacrylamide Gels ◽  
Long Tail

The fine structure of γ phage of Bacillus anthracis was studied by electron microscopy with a negative-staining technique. The phage has a hexagonal head and a long tail without a sheath. By electrophoresis on polyacrylamide gels, the proteins of the phage particles are separate into 10 polypeptides with moleclar weights ranging from 140 000 to 12 000.

Lipid Infusions in Man

1973 ◽  
Vol 29 (02) ◽  
pp. 450-460 ◽  
Author(s):  
T Hovig ◽  
K. A Grøttum
Keyword(s):  
Electron Microscopy ◽  
Lipid Emulsion ◽  
Staining Technique ◽  
Negative Staining ◽  
Scanning Electron Micrographs ◽  
Lipid Particles ◽  
Ultrathin Sections ◽  
Small Platelet ◽  
Scanning Electron ◽  
Platelet Shape

SummaryThe soybean lipid emulsion “Intralipid” was infused in 10 volunteer individuals, and platelets, obtained up to 22 hours after the infusions, were studied by transmission and scanning electron microscopy. Using negative staining technique, platelet coating with lipid particles could be demonstrated. Platelet engulf ment of the lipid particles was found in ultrathin sections. The lipid particles appeared to enter the platelets via invaginations of the surface membranes and were located in vacuoles and in’ ‘humps”. The platelet shape and internal structure was strikingly well preserved and no evidence of platelet aggregation was demonstrated. Small platelet cytoplasmic fragments containing lipid were observed, and it is suggested that these may be pinched off from the platelets. Both with negative staining and in scanning electron micrographs occasional clumps of lipid particles were observed, and the possibility can not be excluded that such clumps may interfere with microcirculatory flow.

Surface Ultrastructure of Gram (−) Bacteria: I. Discrimination Between Gram (+) and Gram (−) Cells by Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 176-177
Author(s):  
I. L. Uydess
Keyword(s):  
Electron Microscopy ◽  
Surface Topography ◽  
Smooth Surface ◽  
Osmium Tetroxide ◽  
Staining Technique ◽  
Negative Staining ◽  
Surface Ultrastructure

A modified negative staining technique (Osmium Tetroxide-Phosphotungstate procedure; OTPT) allows a detailed visualization and differentiation of the surface topography of Gram (+) and Gram (−) bacteria. All of the Gram (−) organisms studied exhibited a highly convoluted surface distinct from the apparently smooth surface of Gram (+) cells.

scholarly journals Papillomatosis in chaffinches (Fringilla coelebs) in the Czech Republic and Germany

2012 ◽  
Vol 48 (No. 6) ◽  
pp. 169-174 ◽  
Author(s):  
I. Literák ◽  
B. Šmíd ◽  
L. Valíček
Keyword(s):  
Electron Microscopy ◽  
Czech Republic ◽  
Staining Technique ◽  
Skin Lesions ◽  
Negative Staining ◽  
The Czech Republic ◽  
First Report ◽  
Fringilla Coelebs ◽  
Direct Observations

Analyses of ornithological field records, direct observations of typical lesions, and examination by electron microscopy revealed leg papillomatosis in six chaffinches (Fringilla coelebs) in theCzech Republic and one chaffinch inGermany. Papillomavirus was identified by electron microscopy using the negative staining technique in skin lesions of one chaffinch caught in theCzechRepublic. This is the first report of papillomatosis in chaffinches recorded in theCzechRepublic.

Recent Applications of High Resolution Electron Microscopy to Crystalline Arrays of Virus Particles

1978 ◽  
Vol 36 (3) ◽  
pp. 470-482 ◽  
Author(s):  
R.W. Horne
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Analytical Techniques ◽  
Staining Technique ◽  
High Resolution Electron Microscopy ◽  
Optical Diffraction ◽  
Full Potential ◽  
Virus Particles ◽  
Resolution Electron ◽  
Wide Range

The technique of surrounding virus particles with a neutralised electron dense stain was described at the Fourth International Congress on Electron Microscopy, Berlin 1958 (see Home & Brenner, 1960, p. 625). For many years the negative staining technique in one form or another, has been applied to a wide range of biological materials. However, the full potential of the method has only recently been explored following the development and applications of optical diffraction and computer image analytical techniques to electron micrographs (cf. De Hosier & Klug, 1968; Markham 1968; Crowther et al., 1970; Home & Markham, 1973; Klug & Berger, 1974; Crowther & Klug, 1975). These image processing procedures have allowed a more precise and quantitative approach to be made concerning the interpretation, measurement and reconstruction of repeating features in certain biological systems.

Three-Dimensional Reconstruction of Native Androctonus Australis Hemocyanin

1990 ◽  
Vol 48 (1) ◽  
pp. 452-453
Author(s):  
Nicolas Boisset ◽  
Jean-Christophe Taveau ◽  
Jean Lamy ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Solid Body ◽  
Body Surface ◽  
Three Dimensional ◽  
Staining Technique ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
View Reconstruction ◽  
Surface Representations ◽  
Top View

Hemocyanin, the respiratory pigment of the scorpion Androctonus australis is composed of 24 kidney shaped subunits. A model of architecture supported by many indirect arguments has been deduced from electron microscopy (EM) and immuno-EM. To ascertain, the disposition of the subunits within the oligomer, the 24mer was submitted to three-dimensional reconstruction by the method of single-exposure random-conical tilt series.A sample of native hemocyanin, prepared with the double layer negative staining technique, was observed by transmisson electron microscopy under low-dose conditions. Six 3D-reconstructions were carried out indenpendently from top, side and 45°views. The results are composed of solid-body surface representations, and slices extracted from the reconstruction volume.The main two characters of the molecule previously reported by Van Heel and Frank, were constantly found in the solid-body surface representations. These features are the presence of two different faces called flip and flop and a rocking of the molecule around an axis passing through diagonnally opposed hexamers. Furthermore, in the solid-body surface of the top view reconstruction, the positions and orientations of the bridges connecting the half molecules were found in excellent agreement with those predicted by the model.

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