False-positive reactions with enzyme-linked immunosorbent assay of Mycobacterium tuberculosis antigens in pleural fluid

1988 ◽  
Vol 26 (4) ◽  
pp. 241-243 ◽  
Author(s):  
R. Dhand ◽  
N. K. Ganguly ◽  
C. Vaishnavi ◽  
R. Gilhotra ◽  
S. K. Malik
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pleural Fluid ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Enzyme Linked Immunosorbent Assay ◽  
False Positive Reactions

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

scholarly journals Improved Sensitivity of Diagnosis of Tuberculosis in Patients in Korea via a Cocktail Enzyme-Linked Immunosorbent Assay Containing the Abundantly Expressed Antigens of the K Strain of Mycobacterium tuberculosis

2008 ◽  
Vol 15 (12) ◽  
pp. 1788-1795 ◽  
Author(s):  
A-Rum Shin ◽  
Sung Jae Shin ◽  
Kil-Soo Lee ◽  
Sun-Ho Eom ◽  
Seung-Sub Lee ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Immunosorbent Assay ◽  
Area Under The Curve ◽  
Infectious Agent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Characteristic Analysis ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Culture Filtrates ◽  
Single Antigen

ABSTRACT Tuberculosis (TB) is the leading cause of death from a single infectious agent in Korea. In this study, we compared the proteins present in culture filtrates from Mycobacterium tuberculosis strain K, which is the dominant clinical isolate in Korea, with those present in culture filtrates from M. tuberculosis H37Rv. Several differences in expression were detected between the two strains for those proteins with a molecular mass of <20 kDa. ESAT-6, HSP-X, and CFP-10 were found to be abundantly expressed in the strain K culture filtrates by liquid chromatography-electrospray ionization-time of flight mass spectrometry. The serodiagnostic potentials of recombinant antigens rESAT-6, rHSP-X, and rCFP-10 and two native antigens (Ag85 and PstS1) were evaluated by Western blot analysis and enzyme-linked immunosorbent assay (ELISA) using sera collected from 46 TB patients with active disease and 46 healthy controls. As for our ELISA results, HSP-X was superior to the other antigens in terms of sensitivity when a single antigen was employed. The results of a receiver operator characteristic analysis revealed that a cocktail ELISA using all five antigens was significantly more sensitive (77.8%) than the use of a single antigen and offered equivalent specificity; moreover, it produced the largest area under the curve (0.91 versus 0.55 to 0.87). Therefore, a cocktail ELISA containing abundantly expressed antigens enhances the sensitivity of a single antigen and can be a useful diagnostic tool for the detection of active TB.

scholarly journals High Frequency of False-Positive Hepatitis C Virus Enzyme-Linked Immunosorbent Assay in Rakai, Uganda

2013 ◽  
Vol 57 (12) ◽  
pp. 1747-1750 ◽  
Author(s):  
C. E. Mullis ◽  
O. Laeyendecker ◽  
S. J. Reynolds ◽  
P. Ocama ◽  
J. Quinn ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immunosorbent Assay ◽  
False Positive ◽  
High Frequency ◽  
Enzyme Linked Immunosorbent Assay
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