scholarly journals A hypothetical holoenzyme involved in the replication of the murein sacculus of Escherichia coli

Microbiology ◽  
1996 ◽  
Vol 142 (8) ◽  
pp. 1911-1918 ◽  
Author(s):  
J.-V. Holtje
Keyword(s):  
Escherichia Coli ◽  
Murein Sacculus

scholarly journals Restricted Mobility of Cell Surface Proteins in the Polar Regions of Escherichia coli

2004 ◽  
Vol 186 (9) ◽  
pp. 2594-2602 ◽  
Author(s):  
Miguel A. de Pedro ◽  
Christoph G. Grünfelder ◽  
Heinz Schwarz
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Cell Envelope ◽  
Envelope Proteins ◽  
Surface Proteins ◽  
Polar Regions ◽  
Free Medium ◽  
Cell Surface Proteins ◽  
Restricted Mobility ◽  
Murein Sacculus

ABSTRACT The polar regions of the Escherichia coli murein sacculus are metabolically inert and stable in time. Because the sacculus and the outer membrane are tightly associated, we investigated whether polar inert murein could restrict the mobility of other cell envelope elements. Cells were covalently labeled with a fluorescent reagent, chased in dye-free medium, and observed by microscopy. Fluorescent material was more efficiently retained at the cell poles than at any other location. The boundary between high and low fluorescence intensity areas was rather sharp. Labeled material consisted mostly of cell envelope proteins, among them the free and murein-bound forms of Braun's lipoprotein. Our results indicate that the mobility of at least some cell envelope proteins is restrained at regions in correspondence with underlying areas of inert murein.

scholarly journals Interactions of Escherichia coli membrane lipoproteins with the murein sacculus.

1992 ◽  
Vol 174 (24) ◽  
pp. 7982-7988 ◽  
Author(s):  
M Leduc ◽  
K Ishidate ◽  
N Shakibai ◽  
L Rothfield
Keyword(s):  
Escherichia Coli ◽  
Murein Sacculus ◽  
Membrane Lipoproteins

scholarly journals Growth pattern of the murein sacculus of Escherichia coli.

1990 ◽  
Vol 265 (31) ◽  
pp. 18988-18996 ◽  
Author(s):  
B Glauner ◽  
J.V. Höltje
Keyword(s):  
Escherichia Coli ◽  
Growth Pattern ◽  
Murein Sacculus
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