scholarly journals Gynuella sunshinyii gen. nov., sp. nov., an antifungal rhizobacterium isolated from a halophyte, Carex scabrifolia Steud

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 1038-1043 ◽  
Author(s):  
Eu Jin Chung ◽  
Jeong Ae Park ◽  
Che Ok Jeon ◽  
Young Ryun Chung
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Sequence Similarities

An antifungal bacterial strain, designated YC6258T, was isolated from the rhizosphere of a halophyte (Carex scabrifolia Steud.) growing in a tidal flat area of Namhae Island, Korea. Cells of the strain were Gram-stain-negative, facultatively anaerobic, moderately halophilic, rod-shaped and motile by a single polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC6258T formed a phyletic lineage distinct from members of the most closely related genera, Saccharospirillum and Reinekea, with less than 91.2 % sequence similarities. The major cellular fatty acids were C18 : 1ω7c, C16 : 0 and Summed feature 3 (C16 : 1ω7c/ C16 : 1ω6c). The quinone system of strain YC6258T consisted mainly of ubiquinone Q-8. The polar lipid profile exhibited phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unknown lipids. The DNA G+C content was 48.9 mol%. Based on the phylogenetic and phenotypic characteristics, strain YC6258T should be classified as a representative of a novel species in a novel genus for which the name Gynuella sunshinyii gen. nov., sp. nov. is proposed. The type strain is YC6258T (KCCM 43015T = NBRC 109345T).

scholarly journals Flavisolibacter rigui sp. nov., isolated from freshwater of an artificial reservoir and emended description of the genus Flavisolibacter

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4038-4042 ◽  
Author(s):  
Keun Sik Baik ◽  
Mi Sun Kim ◽  
Ji Hee Lee ◽  
Sang Suk Lee ◽  
Wan-Taek Im ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A non-motile and rod-shaped bacterium, designated strain 02SUJ3T, was isolated from freshwater collected from the Juam Reservoir (Republic of Korea). Cells were Gram-stain-negative, aerobic, oxidase-negative and catalase-positive. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The strain contained MK-7 as the major isoprenoid quinone. The main polar lipid was phosphatidylethanolamine. The DNA G+C content was 46.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 02SUJ3T forms an independent lineage within the genus Flavisolibacter with low sequence similarity to Flavisolibacter ginsengiterrae Gsoil 492T and Flavisolibacter ginsengisoli Gsoil 643T (95.7 %). Phenotypic characteristics distinguished strain 02SUJ3T from members of the genus Flavisolibacter . On the basis of the evidence presented in this study, strain 02SUJ3T is considered to represent a novel species, for which the name Flavisolibacter rigui sp. nov. is proposed. The type strain is 02SUJ3T ( = JCM 17515T = KCTC 23328T). An emended description of the genus Flavisolibacter is also provided.

scholarly journals Novosphingobium ovatum sp. nov., isolated from a freshwater mesocosm

2020 ◽  
Vol 70 (10) ◽  
pp. 5243-5254 ◽  
Author(s):  
Wen-Ming Chen ◽  
Cheng-Ye Cai ◽  
Der-Shyan Sheu ◽  
Jyh-Ming Tsai ◽  
Shih-Yi Sheu
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A bacterial strain, designated FSY-8T, was isolated from a freshwater mesocosm in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain FSY-8T were aerobic, Gram-stain-negative, rod-shaped, non-motile and formed yellow coloured colonies on Reasoner's 2A agar. Growth occurred at 20–40 °C (optimum, 30–37 °C) and pH 5–7 (optimum, pH 6) and in the presence of 0–0.5 % NaCl (optimum, 0 %, w/v). The major fatty acids (>10 %) of strain FSY-8T were summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c) and summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, diphosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized glycolipid and an uncharacterized lipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 64.8 mol %. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain FSY-8T formed a phylogenetic lineage in the genus Novosphingobium . Strain FSY-8T showed 71.6–77.2 % average nucleotide identity and 19.9–22.8 % digital DNA–DNA hybridization identity with the strains of other Novosphingobium species. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain FSY-8T should be classified in a novel species of the genus Novosphingobium , for which the name Novosphingobium ovatum sp. nov. is proposed. The type strain is FSY-8T (=BCRC 81051T=LMG 30053T=KCTC 52812T).

Flavobacterium fontis sp. nov., isolated from freshwater

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1653-1657 ◽  
Author(s):  
Jeesun Chun ◽  
Ji Young Kang ◽  
Kwang Yeop Jahng
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Freshwater Pond ◽  
The Family ◽  
The 16S Rrna Gene

A taxonomic study was carried out on a novel bacterial strain, designated MIC3010T, which was isolated from a freshwater pond in Jeonju, Republic of Korea. Cells of the isolate were Gram-stain-negative, rod-shaped and non-motile. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the family Flavobacteriaceae , with Flavobacterium haoranii LQY-7T as its closest relative, with a similarity of 94.2 %. The predominant fatty acids of strain MIC3010T were iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipid profile of strain MIC3010T revealed the presence of phosphatidylethanolamine (PE) and one unidentified lipid (L1) as major components. In addition, two aminolipids (AL1, AL2) and one glycolipid were present in small amounts. The DNA G+C content of the strain was 41.0 mol%. The strain contained MK-6 as the major quinone and sym-homospermidine as the predominant polyamine. On the basis of the evidence presented, it is concluded that strain MIC3010T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium fontis sp. nov. is proposed. The type strain is MIC3010T ( = KACC 16593T = JCM 18212T).

scholarly journals Luteimicrobium xylanilyticum sp. nov., isolated from the gut of a long-horned beetle, Massicus raddei

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1401-1405 ◽  
Author(s):  
Hyangmi Kim ◽  
Hyun-Woo Oh ◽  
Ji-Ae Kim ◽  
Doo-Sang Park ◽  
Hee-Moon Park ◽  
...  
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Horned Beetle ◽  
Unknown Lipid ◽  
Unknown Phospholipid

A novel strain, designated W-15T, was isolated from the gut of a long-horned beetle, Massicus raddei, collected in South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the suborder Micrococcineae . Strain W-15T was most closely related to Luteimicrobium album RI148-Li105T (97.9 % similarity). Strain W-15T was Gram-stain-positive, rod- and coccus-shaped and non-motile. Growth was observed at 15–37 °C, at pH 4.5–8.5 and in the presence of 0–5.0 % NaCl. The cell-wall peptidoglycan of the strain was A4α (l-Lys–d-Ser–d-Asp). The major menaquinone present in this strain was MK-8 (H2) and the major cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C17 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown lipid, an unknown phospholipid and an unknown phosphoglycolipid. The G+C content of genomic DNA of the strain was 73.8 mol%. On the basis of evidence from our polyphasic taxonomic study, strain W-15T is classified as representing a novel species in the suborder Micrococcineae , for which the name Luteimicrobium xylanilyticum sp. nov. is proposed. The type strain of this species is strain W-15T ( = KCTC 19882T = JCM 18090T).

scholarly journals Description of a Gram-negative bacterium, Sphingomonas guangdongensis sp. nov.

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1697-1702 ◽  
Author(s):  
Guang-Da Feng ◽  
Song-Zhen Yang ◽  
Yong-Hong Wang ◽  
Xiu-Xiu Zhang ◽  
Guo-Zhen Zhao ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Lead Zinc ◽  
Pr China ◽  
Sequence Similarities

A Gram-stain-negative bacterial strain, designated 9NM-8T, was isolated from an abandoned lead-zinc ore in Mei county, Meizhou, Guangdong province, PR China. The isolate was orange-pigmented, aerobic, oxidase- and catalase-positive, motile with lophotrichous flagella and rod-shaped. Strain 9NM-8T grew optimally at pH 7.0 and 30 °C and in the absence of NaCl on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 9NM-8T belongs to the genus Sphingomonas , with highest sequence similarities to Sphingomonas azotifigens KACC 14484T (96.1 %), Sphingomonas trueperi DSM 7225T (96.0 %) and Sphingomonas pituitosa DSM 13101T (95.6 %). Strain 9NM-8T contained Q-10 as the predominant ubiquinone. The major fatty acids included C18 : 1ω7c, C16 : 0, C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3) and 11-methyl C18 : 1ω7c. The DNA G+C content was 69.6±1.3 mol%. The major component in the polyamine pattern was sym-homospermidine and the polar lipid profile contained sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and two unidentified phospholipids. Based on comparative analysis of physiological, chemotaxonomic and phylogenetic characteristics, strain 9NM-8T should be considered to represent a novel species of the genus Sphingomonas , for which the name Sphingomonas guangdongensis sp. nov. is proposed. The type strain is 9NM-8T ( = GIMCC 1.653T = CGMCC 1.12672T = DSM 27570T).

scholarly journals Muriicola marianensis sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 407-411 ◽  
Author(s):  
Jing Hu ◽  
Wei-Yan Zhang ◽  
Xin-Qi Zhang ◽  
Hong-Cheng ◽  
Xu-Fen Zhu ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8T, was isolated from seawater of the Mariana Trench. The isolate grew at 4–50 °C (optimum 30–35 °C), at pH 6.5–8.0 (optimum pH 7.5) and with 0.5–4.0 % (w/v) NaCl (optimum 1.0–2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8T was related most closely to the genus Muriicola and shared highest sequence similarity of 97.7 % with Muriicola jejuensis EM44T. Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8T included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA–DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8T and M. jejuensis EM44T were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8T ( = CGMCC 1.12606T = KCTC 32436T) is considered to be the type strain of a novel species of the genus Muriicola , for which the name Muriicola marianensis sp. nov. is proposed.

scholarly journals Arthrobacter liuii sp. nov., resuscitated from Xinjiang desert soil

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 896-901 ◽  
Author(s):  
Xiao-Yun Yu ◽  
Li Zhang ◽  
Biao Ren ◽  
Na Yang ◽  
Mei Liu ◽  
...  
Keyword(s):  
Novel Species ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Diamino Acid

A Gram-stain positive, aerobic, non-motile actinobacterium, designated DSXY973T, was isolated from soil samples collected from Xinjiang desert using medium supplemented with resuscitation-promoting factor, and subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that DSXY973T belonged to the genus Arthrobacter and was most closely related to Arthrobacter oryzae JCM 15922T with 97.1 % similarity. The DNA G+C content was 67.6 %. Cells of strain DSXY973T mainly contained MK-9(H2), and the cell wall contained l-lysine as the primary diamino acid. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. Strain DSXY973T was positive for catalase and negative for oxidase activity. On the basis of its phylogenetic position and phenotypic properties, strain DSXY973T represents a novel species of the genus Arthrobacter , for which the name Arthrobacter liuii sp. nov. is proposed. The type strain is DSXY973T ( = CGMCC1.12778T = JCM 19864T).

Pontibacter ramchanderi sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 2829-2834 ◽  
Author(s):  
Amit Kumar Singh ◽  
Nidhi Garg ◽  
Naseer Sangwan ◽  
Vivek Negi ◽  
Roshan Kumar ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Soil Sediment ◽  
Genotypic Characteristics ◽  
Sequence Similarities

A Gram-stain-negative, motile, red pigmented, rod-shaped bacterium, designated strain LP43T, was isolated from hexachlorocyclohexane (HCH)-contaminated soil sediment (Lucknow, India). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate formed a cluster with the genus Pontibacter in the phylum Bacteroidetes with sequence similarities ranging from 92.9 to 97.0 % with species of the genus Pontibacter . The DNA G+C content of strain LP43T was 59.1 mol%. The polar lipid profile of strain LP43T showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids and unknown polar lipids. Strain LP43T contained MK-7 as the predominant menaquinone and sym-homospermidine as the major polyamine. The major cellular fatty acids of strain LP43T were, iso-C15 : 0 (15.74 %), iso-C15 : 0 3-OH (7.57 %), iso-C17 : 0 3-OH (7.32 %), summed feature 4 (iso-C17 : 1 I/anteiso-C17 : 1 B) (31.22 %) and summed feature 8 (C18 : 1ω7c/ C18 : 1ω6c) (7.60 %). Based on the results of DNA–DNA hybridization and phenotypic and genotypic characteristics, strain LP43T represents a novel species of the genus Pontibacter , for which the name Pontibacter ramchanderi is proposed. The type strain is LP43T ( = CCM 8406T = MCC 2019T).

scholarly journals Clostridium algifaecis sp. nov., an anaerobic bacterial species from decomposing algal scum

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3844-3848 ◽  
Author(s):  
Yu-Fan Wu ◽  
Hui Zheng ◽  
Qing-long Wu ◽  
Hong Yang ◽  
Shuang-Jiang Liu
Keyword(s):  
Novel Species ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Clostridium Tyrobutyricum ◽  
Gram Stain ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type

Two anaerobic bacterial strains, MB9-7T and MB9-9, were isolated from decomposing algal scum and were characterized using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences showed that strains MB9-7T and MB9-9 are closely related to each other (99.7 % similarity) and they are also closely related to Clostridium tyrobutyricum (96.5 %). The two strains were Gram-stain positive and rod-shaped. Growth occurred at 20–45 °C, at pH 4.0–8.0 and at NaCl concentrations of up to 2 % (w/v). Acid was produced from glucose, xylose and mannose. Products of fermentation in PYG medium were mainly butyrate, acetate, carbon dioxide and hydrogen. The predominant cellular fatty acids were C14 : 0 and C16 : 0. The cellular polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two glycolipids, one phospholipid, one aminophospholipid and two aminolipids. The DNA G+C contents of strain MB9-7T and MB9-9 were 27.9 and 28.7 mol%, respectively. These results support the assignment of the new isolates to the genus Clostridium and also distinguish them from other species of the genus Clostridium . Hence, it is proposed that strains MB9-7T and MB9-9 represent a novel species of the genus Clostridium , with the suggested name Clostridium algifaecis sp. nov. The type strain is MB9-7T ( = CGMCC 1.5188T = DSM 28783T).

scholarly journals Flaviramulus aquimarinus sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 644-649 ◽  
Author(s):  
Ji Hee Lee ◽  
Keun Sik Baik ◽  
Chi Nam Seong
Keyword(s):  
Novel Species ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Sea Salts ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
The Family

A yellow-coloured, rod-shaped, non-motile, Gram-stain-negative and facultative anaerobic bacterium, designated KYW499T, was isolated from seawater collected at Suncheon Bay, Republic of Korea. The isolate required sea salts for growth. Flexirubin-type pigments were absent. Major cellular fatty acids (>10 % of total) were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH. The main polar lipids were phosphatidylethanolamine and two unidentified polar lipids. The predominant isoprenoid quinone was MK-6. The genomic DNA G+C content was 31.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW499T belonged to the family Flavobacteriaceae , and was related to the genus Flaviramulus . Data from a polyphasic taxonomy study suggested that the isolate represents a novel species of the genus Flaviramulus , for which the name Flaviramulus aquimarinus sp. nov. is proposed. The type strain is KYW499T ( = KCTC 23924T = JCM 18274T).

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