Flavobacterium fontis sp. nov., isolated from freshwater

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1653-1657 ◽  
Author(s):  
Jeesun Chun ◽  
Ji Young Kang ◽  
Kwang Yeop Jahng
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Freshwater Pond ◽  
The Family ◽  
The 16S Rrna Gene

A taxonomic study was carried out on a novel bacterial strain, designated MIC3010T, which was isolated from a freshwater pond in Jeonju, Republic of Korea. Cells of the isolate were Gram-stain-negative, rod-shaped and non-motile. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the family Flavobacteriaceae , with Flavobacterium haoranii LQY-7T as its closest relative, with a similarity of 94.2 %. The predominant fatty acids of strain MIC3010T were iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipid profile of strain MIC3010T revealed the presence of phosphatidylethanolamine (PE) and one unidentified lipid (L1) as major components. In addition, two aminolipids (AL1, AL2) and one glycolipid were present in small amounts. The DNA G+C content of the strain was 41.0 mol%. The strain contained MK-6 as the major quinone and sym-homospermidine as the predominant polyamine. On the basis of the evidence presented, it is concluded that strain MIC3010T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium fontis sp. nov. is proposed. The type strain is MIC3010T ( = KACC 16593T = JCM 18212T).

scholarly journals Pedobacter pituitosus sp. nov., isolated from a waterfall

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3838-3843 ◽  
Author(s):  
Jeesun Chun ◽  
Ji Young Kang ◽  
Kwang Yeop Jahng
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Unknown Lipid ◽  
Ph Range ◽  
The 16S Rrna Gene

A taxonomic study was carried out on a novel bacterial strain, designated MIC2002T, which was isolated from Wibong falls in Korea. Cells were Gram-stain-negative, aerobic, non-motile and rods, 0.3–0.5 µm wide and 4.0–5.0 µm long. The optimum temperature and pH range for growth were 25 °C and pH 6.5–7.0, respectively. Catalase and oxidase activities were positive. Flexirubin pigments were not produced. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the genus Pedobacter , with Pedobacter daechungensis as its closest relative, with a similarity of 94.4 %. It contained iso-C15 : 0, anteiso-C15 : 0, C16 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C17 : 0 3-OH as the major fatty acids and menaquinone MK-7 as isoprenoid quinone. The polar lipid profile of strain MIC2002T revealed the presence of phosphatidylethanolamine and an unknown lipid. The DNA G+C content of the strain was 34.7 mol%. On the basis of the evidences presented, it was concluded that strain MIC2002T represents a novel species of the genus Pedobacter within the family Sphingobacteriaceae , for which the name Pedobacter pituitosus sp. nov. is proposed. The type strain is MIC2002T ( = KACC 17064T = JCM 18729T).

Dyadobacter jejuensis sp. nov., isolated from seawater

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1788-1792 ◽  
Author(s):  
Jeesun Chun ◽  
Ji Young Kang ◽  
Yochan Joung ◽  
Haneul Kim ◽  
Kiseong Joh ◽  
...  
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
The 16S Rrna Gene

A taxonomic study was carried out on a novel bacterial strain, designated AM1R11T, which was isolated from seawater of Jeju Island in Korea. Cells of the isolate were found to be Gram-negative, rod-shaped and non-motile. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the family Cytophagaceae , with Dyadobacter ginsengisoli Gsoil 043T as its closest relative, with a similarity of 96.6 %. It contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 36.9 %), iso-C15 : 0 (16.5 %) and C16 : 1ω5c (16.3 %) as the major fatty acids and MK-7 as the predominant menaquinone. The polar lipid profile of strain AM1R11T revealed the presence of phosphatidylethanolamine, one aminolipid and four unidentified lipids (L1, L2, L3 and L4). The DNA G+C content of strain AM1R11T was 45.1 mol%. On the basis of the evidence presented, it is concluded that strain AM1R11T represents a novel species of the genus Dyadobacter , for which the name Dyadobacter jejuensis sp. nov. is proposed. The type strain is AM1R11T ( = KACC 16446T = JCM 17918T).

scholarly journals Novosphingobium ovatum sp. nov., isolated from a freshwater mesocosm

2020 ◽  
Vol 70 (10) ◽  
pp. 5243-5254 ◽  
Author(s):  
Wen-Ming Chen ◽  
Cheng-Ye Cai ◽  
Der-Shyan Sheu ◽  
Jyh-Ming Tsai ◽  
Shih-Yi Sheu
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A bacterial strain, designated FSY-8T, was isolated from a freshwater mesocosm in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain FSY-8T were aerobic, Gram-stain-negative, rod-shaped, non-motile and formed yellow coloured colonies on Reasoner's 2A agar. Growth occurred at 20–40 °C (optimum, 30–37 °C) and pH 5–7 (optimum, pH 6) and in the presence of 0–0.5 % NaCl (optimum, 0 %, w/v). The major fatty acids (>10 %) of strain FSY-8T were summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c) and summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, diphosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized glycolipid and an uncharacterized lipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 64.8 mol %. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain FSY-8T formed a phylogenetic lineage in the genus Novosphingobium . Strain FSY-8T showed 71.6–77.2 % average nucleotide identity and 19.9–22.8 % digital DNA–DNA hybridization identity with the strains of other Novosphingobium species. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain FSY-8T should be classified in a novel species of the genus Novosphingobium , for which the name Novosphingobium ovatum sp. nov. is proposed. The type strain is FSY-8T (=BCRC 81051T=LMG 30053T=KCTC 52812T).

scholarly journals Gynuella sunshinyii gen. nov., sp. nov., an antifungal rhizobacterium isolated from a halophyte, Carex scabrifolia Steud

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 1038-1043 ◽  
Author(s):  
Eu Jin Chung ◽  
Jeong Ae Park ◽  
Che Ok Jeon ◽  
Young Ryun Chung
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Sequence Similarities

An antifungal bacterial strain, designated YC6258T, was isolated from the rhizosphere of a halophyte (Carex scabrifolia Steud.) growing in a tidal flat area of Namhae Island, Korea. Cells of the strain were Gram-stain-negative, facultatively anaerobic, moderately halophilic, rod-shaped and motile by a single polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC6258T formed a phyletic lineage distinct from members of the most closely related genera, Saccharospirillum and Reinekea, with less than 91.2 % sequence similarities. The major cellular fatty acids were C18 : 1ω7c, C16 : 0 and Summed feature 3 (C16 : 1ω7c/ C16 : 1ω6c). The quinone system of strain YC6258T consisted mainly of ubiquinone Q-8. The polar lipid profile exhibited phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unknown lipids. The DNA G+C content was 48.9 mol%. Based on the phylogenetic and phenotypic characteristics, strain YC6258T should be classified as a representative of a novel species in a novel genus for which the name Gynuella sunshinyii gen. nov., sp. nov. is proposed. The type strain is YC6258T (KCCM 43015T = NBRC 109345T).

scholarly journals Muriicola marianensis sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 407-411 ◽  
Author(s):  
Jing Hu ◽  
Wei-Yan Zhang ◽  
Xin-Qi Zhang ◽  
Hong-Cheng ◽  
Xu-Fen Zhu ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8T, was isolated from seawater of the Mariana Trench. The isolate grew at 4–50 °C (optimum 30–35 °C), at pH 6.5–8.0 (optimum pH 7.5) and with 0.5–4.0 % (w/v) NaCl (optimum 1.0–2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8T was related most closely to the genus Muriicola and shared highest sequence similarity of 97.7 % with Muriicola jejuensis EM44T. Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8T included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA–DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8T and M. jejuensis EM44T were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8T ( = CGMCC 1.12606T = KCTC 32436T) is considered to be the type strain of a novel species of the genus Muriicola , for which the name Muriicola marianensis sp. nov. is proposed.

Mucilaginibacter jinjuensis sp. nov., with xylan-degrading activity

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1267-1272 ◽  
Author(s):  
Haji Khan ◽  
Eu Jin Chung ◽  
Dong Young Kang ◽  
Che Ok Jeon ◽  
Young Ryun Chung
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Content Type ◽  
Link Type ◽  
Rotten Wood ◽  
The Family ◽  
The 16S Rrna Gene

A Gram-negative, rod-shaped, pale-orange-pigmented bacterial strain with xylan-degrading activity designated YC7004T was isolated from a rotten-wood sample collected at Jinju, Korea, and its taxonomic position was investigated by using a polyphasic approach. The strain grew optimally on R2A medium at 30 °C and at pH 6. The major isoprenoid quinone was MK7 and major fatty acids were summed feature 3, iso-C15 : 0, C16 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and C16 : 1ω5c. The G+C content of the genomic DNA was 40.0 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain belongs to the genus Mucilaginibacter in the family Sphingobacteriaceae . The most closely related species were Mucilaginibacter daejeonensis (95.5 %), Mucilaginibacter frigoritolerans (94.6 %) and Mucilaginibacter mallensis (94.0 %). Based on the phylogenetic and chemotaxonomic data analyses, strain YC7004T represents a novel species of the genus Mucilaginibacter with the proposed name of Mucilaginibacter jinjuensis sp. nov. The type strain is YC7004T ( = KACC 16571T = NBRC 108856T).

Gramella flava sp. nov., a member of the family Flavobacteriaceae isolated from seawater

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 165-168 ◽  
Author(s):  
Keshao Liu ◽  
Shuhui Li ◽  
Nianzhi Jiao ◽  
Kai Tang
Keyword(s):  
Marine Bacterium ◽  
Novel Species ◽  
Rrna Gene ◽  
Surface Seawater ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Motile By Gliding

A novel Gram-negative, yellow-pigmented, aerobic, motile by gliding, rod-shaped marine bacterium (JLT2011T) was isolated from surface seawater. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JLT2011T could be assigned to the genus Gramella and was most closely related to Gramella gaetbulicola , with 96.2 % similarity. The genomic DNA G+C content was 42.1 %. The predominant fatty acids were C16 : 0, iso-C15 : 0, C18 : 0 and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The major menaquinone was MK-6. The major components of the polar lipid profile were phosphatidylglycerol, diphosphatidylglycerol and sphingoglycolipid. On the basis of phenotypic, genotypic and taxonomic data presented, strain JLT2011T is considered to represent a novel species of the genus Gramella , for which the name Gramella flava sp. nov. is proposed; the type strain is JLT2011T ( = CGMCC 1.12375T = LMG 27360T).

scholarly journals Flaviramulus aquimarinus sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 644-649 ◽  
Author(s):  
Ji Hee Lee ◽  
Keun Sik Baik ◽  
Chi Nam Seong
Keyword(s):  
Novel Species ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Sea Salts ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
The Family

A yellow-coloured, rod-shaped, non-motile, Gram-stain-negative and facultative anaerobic bacterium, designated KYW499T, was isolated from seawater collected at Suncheon Bay, Republic of Korea. The isolate required sea salts for growth. Flexirubin-type pigments were absent. Major cellular fatty acids (>10 % of total) were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH. The main polar lipids were phosphatidylethanolamine and two unidentified polar lipids. The predominant isoprenoid quinone was MK-6. The genomic DNA G+C content was 31.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW499T belonged to the family Flavobacteriaceae , and was related to the genus Flaviramulus . Data from a polyphasic taxonomy study suggested that the isolate represents a novel species of the genus Flaviramulus , for which the name Flaviramulus aquimarinus sp. nov. is proposed. The type strain is KYW499T ( = KCTC 23924T = JCM 18274T).

Limosilactobacillus urinaemulieris sp. nov. and Limosilactobacillus portuensis sp. nov. isolated from urine of healthy women

2019 ◽  
Vol 71 (3) ◽  
Author(s):  
Magdalena Ksiezarek ◽  
Teresa Gonçalves Ribeiro ◽  
Joana Rocha ◽  
Filipa Grosso ◽  
Svetlana Ugarcina Perovic ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Healthy Women ◽  
Genome Distance

Two Gram-stain-positive strains, c9Ua_26_MT and c11Ua_112_MT, were isolated from voided urine samples from two healthy women. Comparative 16S rRNA gene sequences demonstrated that these novel strains were members of the genus Limosilactobacillus . Phylogenetic analysis based on pheS gene sequences and core genomes showed that each strain formed a separated branch and are closest to Limosilactobacillus vaginalis DSM 5837T. The average nucleotide identity (ANI) and Genome-to-Genome Distance Calculator (GGDC) values between c9Ua_26_MT and the closest relative DSM 5837T were 90.7 and 42.9 %, respectively. The ANI and GGDC values between c11Ua_112_MT and the closest relative DSM 5837T were 91.2 and 45.0 %, and those among the strains were 92.9% and 51,0 %, respectively. The major fatty acids were C12 : 0 (40.2 %), C16 : 0 (26.7 %) and C18 : 1 ω9c (17.7 %) for strain c9Ua_26_MT, and C18 : 1 ω9c (38.0 %), C16 : 0 (33.3 %) and C12 : 0 (17.6 %) for strain c11Ua_112_MT. The genomic DNA G+C content of strains c9Ua_26_MT and c11Ua_112_MT was 39.9 and 39.7 mol%, respectively. On the basis of the data presented here, strains c9Ua_26_MT and c11Ua_112_MT represent two novel species of the genus Limosilactobacillus , for which the names Limosilactobacillus urinaemulieris sp. nov. (c9Ua_26_MT=CECT 30144T=LMG 31899T) and Limosilactobacillus portuensis sp. nov. (c11Ua_112_MT=CECT 30145T=LMG 31898T) are proposed.

scholarly journals Mesoflavibacter aestuarii sp. nov., a zeaxanthin-producing marine bacterium isolated from seawater

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1932-1937 ◽  
Author(s):  
Ji Hee Lee ◽  
Yeoung Min Hwang ◽  
Keun Sik Baik ◽  
Kap Seong Choi ◽  
Jong-Ok Ka ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Carotenoid Pigment ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Gwangyang Bay

An orange, rod-shaped, Gram-reaction-negative, aerobic and gliding bacterial strain devoid of flagella, designated strain KYW614T, was isolated from seawater collected from Gwangyang Bay, Republic of Korea. Zeaxanthin was the major carotenoid pigment produced and flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW614T belonged to the family Flavobacteriaceae and it was most closely related to Mesoflavibacter zeaxanthinifaciens TD-ZX30T (96.5 %, sequence similarity). The predominant cellular fatty acids of strain KYW614T were iso-C15 : 1 G (10.5 %), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 10.0 %), iso-C15 : 0 (9.5 %), C15 : 0 (7.5 %) and iso-C17 : 0 3-OH (7.4 %). MK-6 was the only isoprenoid quinone and the DNA G+C content was 32.6 mol%. Data from a polyphasic taxonomic study suggested that the isolate represents a novel species in the genus Mesoflavibacter , for which the name Mesoflavibacter aestuarii sp. nov. is proposed. The type strain is KYW614T ( = KCTC 32269T = JCM 19524T).

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