scholarly journals Winogradskyella crassostreae sp. nov., isolated from an oyster (Crassostrea gigas)

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2890-2895 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Sung-Min Won ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Phylogenetic Trees ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
South Sea ◽  
The Mean ◽  
Type Strains

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated TYO-19T, was isolated from an oyster collected from the South Sea in South Korea, and was subjected to a taxonomic study using a polyphasic approach. Strain TYO-19T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0  % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain TYO-19T belonged to the genus Winogradskyella, clustering coherently with the type strain of Winogradskyella epiphytica. Strain TYO-19T exhibited a 16S rRNA gene sequence similarity value of 99.7  % to W. epiphytica KMM 3906T and 94.2–96.9  % to the type strains of other species of the genus Winogradskyella. Strain TYO-19T contained MK-6 as the predominant menaquinone and iso-C15[hairsp] :[hairsp] 1 G, iso-C17[hairsp] :[hairsp] 0 3-OH, iso-C15[hairsp] :[hairsp] 0 and anteiso-C15[hairsp] :[hairsp] 0 as the major fatty acids. The major polar lipids detected in strain TYO-19T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content was 39.0 mol% and the mean DNA–DNA relatedness value with the type strain of W. epiphytica was 59 ± 4.3  %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain TYO-19T is separated from recognized species of the genus Winogradskyella. On the basis of the data presented, strain TYO-19T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella crassostreae sp. nov. is proposed. The type strain is TYO-19T  ( = KCTC 42462T = NBRC 110924T).

scholarly journals Demequina activiva sp. nov., isolated from a tidal flat

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2042-2047 ◽  
Author(s):  
Sooyeon Park ◽  
Yong-Taek Jung ◽  
Sung-Min Won ◽  
Jung-Sook Lee ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Strain ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
South Sea ◽  
Type Strains

A Gram-stain-positive, facultatively anaerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated BS-12MT, was isolated from a tidal flat sediment on the South Sea, South Korea. Strain BS-12MT grew optimally at 35 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain BS-12MT fell within the cluster comprising the type strains of species of the genus Demequina, joining the type strain of Demequina aestuarii with which it shared the highest sequence similarity (98.6 %). It exhibited 16S rRNA gene sequence similarity of 96.1–97.9 % to the type strains of other species of the genus Demequina. The peptidoglycan type of strain BS-12MT was A4β based on l-Orn − l-Ser − d-Glu. Strain BS-12MT contained demethylmenaquinone-9(H4) as the major menaquinone and anteiso-C15:0 and C16:0 as the major fatty acids. The major polar lipids of strain BS-12MT were phosphatidylinositol and phosphatidylinositolmannoside. The DNA G+C content of strain BS-12MT was 70.7 mol% and its DNA–DNA relatedness values with the type strains of five phylogenetically related species of the genus Demequina were 15–34 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain BS-12MT is separate from other species of the genus Demequina. On the basis of the data presented, strain BS-12MT is considered to represent a novel species of the genus Demequina, for which the name Demequina activiva sp. nov. is proposed. The type strain is BS-12MT ( = KCTC 29674T = NBRC 110675T).

scholarly journals Loktanella ponticola sp. nov., isolated from seawater

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3717-3723 ◽  
Author(s):  
Yong-Taek Jung ◽  
Sooyeon Park ◽  
Ji-Min Park ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
South Sea ◽  
Type Strains

A Gram-reaction-negative, aerobic, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, designated W-SW2T, was isolated from seawater in the South Sea of South Korea. The novel strain grew optimally at pH 7.0–8.0, at 25 °C and in the presence of approximately 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain W-SW2T fell within the clade comprising the type strains of species of the genus Loktanella , clustering and sharing the highest sequence similarity value (96.3 %) with the type strain of Loktanella koreensis . The 16S rRNA gene sequence similarity values between strain W-SW2T and the type strains of the other species of the genus Loktanella were in the range 93.1–96.0 %. The DNA G+C content of strain W-SW2T was 55.9 mol%. Strain W-SW2T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the predominant fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain W-SW2T is separated phylogenetically from other species of the genus Loktanella . On the basis of the data presented, strain W-SW2T is considered to represent a novel species of the genus Loktanella , for which the name Loktanella ponticola sp. nov. is proposed. The type strain is W-SW2T ( = KCTC 42133T = NBRC 110409T).

scholarly journals Thalassobius maritimus sp. nov., isolated from seawater

2012 ◽  
Vol 62 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Sooyeon Park ◽  
Mi-Hwa Lee ◽  
Jung-Sook Lee ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
The Mean ◽  
Type Strains

A Gram-stain-negative, aerobic, motile, rod-shaped bacterial strain, GSW-M6T, was isolated from seawater of Geoje island, Korea, and was subjected to a polyphasic taxonomic study. Strain GSW-M6T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GSW-M6T clustered with Thalassobius aestuarii, Thalassobius gelatinovorus and Thalassobius mediterraneus. Strain GSW-M6T exhibited 96.2–96.9 % 16S rRNA gene sequence similarity to the type strains of these three Thalassobius species. Strain GSW-M6T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The polar lipid profiles of strain GSW-M6T and the type strains of the three Thalassobius species were similar, with phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid as common major components. The DNA G+C content of strain GSW-M6T was 57 mol%. The mean level of DNA–DNA relatedness between strain GSW-M6T and the type strain of Thalassobius gelatinovorus was 17 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, enabled strain GSW-M6T to be differentiated from recognized species of the genus Thalassobius. On the basis of the data presented, strain GSW-M6T is considered to represent a novel species of the genus Thalassobius, for which the name Thalassobius maritimus sp. nov. is proposed. The type strain is GSW-M6T ( = KCTC 23347T  = CCUG 60021T).

scholarly journals Marinobacter confluentis sp. nov., a lipolytic bacterium isolated from a junction between the ocean and a freshwater lake

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4873-4879 ◽  
Author(s):  
Sooyeon Park ◽  
Sona Kim ◽  
Chul-Hyung Kang ◽  
Yong-Taek Jung ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Trees ◽  
Sequence Similarity ◽  
Freshwater Lake ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
The Mean ◽  
Type Strains

A Gram-stain-negative, motile, aerobic and rod-shaped bacterium, designated HJM-18T, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain HJM-18T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain HJM-18T belonged to the genus Marinobacter. Strain HJM-18T exhibited 16S rRNA gene sequence similarity values of 97.05–98.22 % to the type strains of Marinobacter algicola, Marinobacter flavimaris, Marinobacter adhaerens, Marinobacter salarius, Marinobacter salsuginis, Marinobacter guineae and Marinobacter gudaonensis and of 93.21–96.98 % to the type strains of the other species of the genus Marinobacter. Strain HJM-18T contained Q-9 as the predominant ubiquinone and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω9c as the major fatty acids. The major polar lipids detected in strain HJM-18T were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminophospholipid. The DNA G+C content was 58 mol% and the mean DNA–DNA relatedness values with the type strains of the seven phylogenetically related species of the genus Marinobacter were 10–27 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain HJM-18T is separated from recognized species of the genus Marinobacter. On the basis of the data presented, strain HJM-18T represents a novel species of the genus Marinobacter, for which the name Marinobacter confluentis sp. nov. is proposed. The type strain is HJM-18T ( = KCTC 42705T = NBRC 111223T).

scholarly journals Pedobacter lignilitoris sp. nov., isolated from wood falls

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3481-3486 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Yong-Taek Jung ◽  
Sung-Min Won ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
The Novel ◽  
Phenotypic Properties ◽  
South Sea ◽  
Type Strains

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated W-WS13T, was isolated from wood falls collected around Wando, an island in the South Sea of South Korea, and subjected to a polyphasic taxonomic study. Strain W-WS13T grew optimally at 30 °C, at pH 7.5 and in the presence of 0.5 % NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain W-WS13T belonged to the genus Pedobacter, clustering robustly with the type strain of Pedobacter arcticus, sharing 95.9 % sequence similarity. Strain W-WS13T exhibited 16S rRNA gene sequence similarity of 90.1–95.6 % to the type strains of the other species of the genus Pedobacter. Strain W-WS13T contained MK-7 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipid detected in strain W-WS13T was phosphatidylethanolamine. The DNA G+C content of strain W-WS13T was 36.9 mol%. Phylogenetic distinctiveness and differential phenotypic properties of strain W-WS13T revealed that the novel strain is separated from recognized species of the genus Pedobacter. On the basis of the data presented, strain W-WS13T is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter lignilitoris sp. nov. is proposed. The type strain is W-WS13T ( = KCTC 42500T = CECT 8725T).

scholarly journals Lutibacter crassostreae sp. nov., isolated from oyster

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2689-2695 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Sung-Min Won ◽  
Doo-Sang Park ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
South Sea ◽  
Type Strains

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and ovoid or rod-shaped bacterial strain, designated TYO-8T, was isolated from an oyster collected from the South Sea in South Korea. Strain TYO-8T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain TYO-8T fell within the clade comprising the type strains of species of the genus Lutibacter, clustering coherently with the type strain of Lutibacter litoralis with a sequence similarity of 99.3 %. Strain TYO-8T exhibited 16S rRNA gene sequence similarity values of 95.3–97.5 % to the type strains of other species of the genus Lutibacter and of less than 92.9 % to the type strains of other species with validly published names. Strain TYO-8T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 0 3-OH and iso-C15 : 1 G as the major fatty acids. The major polar lipids of strain TYO-8T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain TYO-8T was 33.8 mol% and its DNA–DNA relatedness values with the type strains of L. litoralis, Lutibacter aestuarii and Lutibacter flavus were 13–27 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain TYO-8T is distinct from other species of the genus Lutibacter. On the basis of the data presented, strain TYO-8T is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter crassostreae sp. nov. is proposed. The type strain is TYO-8T ( = KCTC 42461T = NBRC 110923T).

Winogradskyella litorisediminis sp. nov., isolated from coastal sediment

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1793-1799 ◽  
Author(s):  
Chul-Hyung Kang ◽  
Soo-Young Lee ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Trees ◽  
Sequence Similarity ◽  
Coastal Sediment ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Type Strains

A Gram-staining-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated DPS-8T, was isolated from coastal sediment of Geoje island in the South Sea, South Korea, and subjected to a polyphasic study. Strain DPS-8T grew optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DPS-8T joined the clade comprising the type strains of Winogradskyella species with a high bootstrap resampling value of 93.5 %. Phylogenetic trees constructed using maximum-likelihood and maximum-parsimony algorithms revealed that strain DPS-8T belonged to the genus Winogradskyella . Strain DPS-8T exhibited 94.1–96.5 % 16S rRNA gene sequence similarity to the type strains of species of the genus Winogradskyella . Strain DPS-8T contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH and C16 : 1ω7c and/or iso-C15 : 0 2-OH as the major fatty acids. The major polar lipids of strain DPS-8T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain DPS-8T was 34.7 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, revealed that strain DPS-8T is separate from recognized species of the genus Winogradskyella . On the basis of the data presented, strain DPS-8T is considered to represent a novel species of the genus Winogradskyella , for which the name Winogradskyella litorisediminis sp. nov. is proposed. The type strain is DPS-8T ( = KCTC 32110T = CCUG 62215T).

Description of Sulfitobacter sediminilitoris sp. nov., isolated from a tidal flat

2020 ◽  
Vol 367 (9) ◽  
Author(s):  
Sooyeon Park ◽  
Siyu Chen ◽  
Jung-Sook Lee ◽  
Wonyong Kim ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Type Strain ◽  
Sequence Similarity ◽  
Genomic Analysis ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Type Strains

ABSTRACT A Gram-stain-negative bacterial strain, JBTF-M27T, was isolated from a tidal flat from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M27T fell within the clade comprising the type strains of Sulfitobacter species. Strain JBTF-M27T exhibited the highest 16S rRNA gene sequence similarity (98.8%) to the type strain of S. porphyrae. Genomic ANI and dDDH values of strain JBTF-M27T between the type strains of Sulfitobacter species were less than 76.1 and 19.2%, respectively. Mean DNA-DNA relatedness value between strain JBTF-M27T and the type strain of S. porphyrae was 21%. DNA G + C content of strain JBTF-M27T from genome sequence was 57.8% (genomic analysis). Strain JBTF-M27T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid. The major polar lipids of strain JBTF-M27T were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M27T is separated from recognized Sulfitobacter species. On the basis of the data presented, strain JBTF-M27T ( = KACC 21648T = NBRC 114356T) is considered to represent a novel species of the genus Sulfitobacter, for which the name Sulfitobacter sediminilitoris sp. nov. is proposed.

scholarly journals Algoriphagus aestuarii sp. nov., a member of the Cyclobacteriaceae isolated from a tidal-flat sediment of the Yellow Sea in Korea

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3439-3446 ◽  
Author(s):  
Yong-Taek Jung ◽  
Jung-Sook Lee ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Tidal Flat Sediment ◽  
The Mean ◽  
Type Strains

A Gram-strain-negative, coccoid or oval-shaped, non-motile bacterial strain, designated MDM-1T, was isolated from a tidal-flat sediment on the Korean peninsula. Strain MDM-1T was found to grow optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MDM-1T falls within the clade comprising species of the genus Algoriphagus, clustering with the type strains of Algoriphagus halophilus, A. lutimaris, A. chungangensis and A. machipongonensis, with which it exhibited 97.2–98.5 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of the other recognized species of the genus Algoriphagus were 92.8–97.6 %. Strain MDM-1T was found to contain MK-7 as the predominant menaquinone and iso-C15 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) as the major fatty acids. The major polar lipids were identified as phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain MDM-1T was determined to be 42.7 mol% and the mean DNA–DNA relatedness with A. halophilus KCTC 12051T, A. lutimaris S1-3T, A. chungangensis KCTC 23759T, A. machipongonensis DSM 24695T and A. ratkowskyi CIP 107452T was 19.7–5.2 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain MDM-1T is distinguishable from recognized species of the genus Algoriphagus. On the basis of the data presented, strain MDM-1T is proposed to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus aestuarii sp. nov. is proposed. The type strain is MDM-1T ( = KCTC 42199T = NBRC 110552T).

scholarly journals Polaribacter undariae sp. nov., isolated from a brown alga reservoir

2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1679-1685 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Yong-Taek Jung ◽  
Kang Hyun Lee ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Brown Alga ◽  
Type Strain ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Type Strains

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped or ovoid bacterial strain, designated W-BA7T, was isolated from a brown alga reservoir on the South Sea in South Korea. This strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain W-BA7T belonged to the genus Polaribacter , clustering coherently with the type strain of Polaribacter sejongensis, showing 99.3 % sequence similarity. Strain W-BA7T exhibited 16S rRNA gene sequence similarity of 93.4–98.6 % to the type strains of the other species of the genus Polaribacter . Strain W-BA7T contained MK-6 as the predominant menaquinone and iso-C15 : 0 3-OH and anteiso-C15 : 0 as the major fatty acids. The major polar lipids of strain W-BA7T were phosphatidylethanolamine, three unidentified lipids, one unidentified glycolipid, one unidentified aminolipid and one unidentified aminophospholipid. The DNA G+C content of strain W-BA7T was 31.9 mol%, and the mean DNA–DNA relatedness with the type strains of four phylogenetically related species of the genus Polaribacter was 12–27 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain W-BA7T is separated from recognized species of the genus Polaribacter . On the basis of the data presented, strain W-BA7T is considered to represent a novel species of the genus Polaribacter , for which the name Polaribacter undariae sp. nov. is proposed. The type strain is W-BA7T ( = KCTC 42175T = CECT 8670T).

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