scholarly journals Lentibacter algarum gen. nov., sp. nov., isolated from coastal water during a massive green algae bloom

2012 ◽  
Vol 62 (Pt_5) ◽  
pp. 1042-1047 ◽  
Author(s):  
Zhao Li ◽  
Zhe Qu ◽  
Xiuming Zhang ◽  
Xiao-Hua Zhang
Keyword(s):  
Green Algae ◽  
Type Strain ◽  
New Genus ◽  
Coastal Region ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Algae Bloom ◽  
Content Type ◽  
The Family

Two novel Gram-stain-negative, aerobic strains (ZXM100T and ZXM098) were isolated from seawater in the coastal region of Qingdao (36.027° N 120.184° E), China, during a massive green algae bloom. Cells were ovoid to irregular short rods and lacked flagella. Poly-β-hydroxybutyrate was accumulated. Cells did not contain bacteriochlorophyll a. The isolates grew at NaCl concentrations of 3–9 % and 22–28 °C. C18 : 1ω7c, C18 : 0 and C16 : 0 were the major fatty acid components. The polar lipids of ZXM100T were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, a single lipid and an unidentified aminolipid. The predominant isoprenoid quinone of ZXM100T was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains belonged to the family Rhodobacteraceae . Based on the results obtained in this study, strain ZXM100T was considered to represent a novel species in a new genus, for which the name Lentibacter algarum gen. nov., sp. nov. is proposed. The type strain is ZXM100T ( = LMG 24861T = CGMCC 1.10234T); the DNA G+C content of the type strain is 54.6 mol%.

Jinshanibacter, a new genus of Budviciaceae: identification of Jinshanibacter zhutongyuii sp. nov. and Jinshanibacter xujianqingii sp. nov. isolated from cloacal content of snow finch (Montifringilla taczanowskii)

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Yajun Ge ◽  
Bin Wang ◽  
Jing Yang ◽  
Xin-He Lai ◽  
Gui Zhang ◽  
...  
Keyword(s):  
Type Strain ◽  
Type Species ◽  
New Genus ◽  
Major Fatty Acid ◽  
Phylogenomic Analysis ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
The Family

Four novel strains isolated from the cloacal contents of snow finches (Montifringilla taczanowskii) were characterized as aerobic, Gram-stain-negative, slightly motile, and rod-shaped. Analysis of the 16S rRNA gene sequence revealed that strain CF-458T had the highest similarities of 96.9 and 96.4 % with Limnobaculum parvum HYN0051T and Pragia fontium DSM 5563T, while strain CF-1111T shared the highest similarities of 96.4 and 96.1 % with Pantoea rodasii LMG 26273T and Pectobacterium punjabense SS95T. Phylogenomic analysis showed the four isolates were separated into group Ⅰ (CF-458T and CF-917) and group Ⅱ (CF-1111T and CF-509), and clustered independently in the vicinity of the genera Limnobaculum and Pragia . Summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c, 23.9 and 17.2 %, respectively), C16 : 0 (21.8 and 22.1 %, respectively) and C14 : 0 (10.6 and 17.7 %, respectively) were the common major fatty acids, and summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c, 12.3 %) was also a major fatty acid for strain CF-458T while cyclo-C17 : 0 (13.1%) was for strain CF-1111T. Both had Q-8 as the sole quinone and contained phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as the major polar lipids. The DNA G+C content of strains CF-458T and CF-1111T was 45.7 and 45.4 mol%, respectively. Based on taxonomic position in the phylogenomic tree and phenotypic properties, two novel species of a new genus within the family Budviciaceae are thus proposed, with the name Jinshanibacter gen. nov., zhutongyuii sp. nov. (type strain CF-458T=CGMCC 1.16483T=GDMCC 1.1586T=JCM 33489T) and Jinshanibacter xujianqingii sp. nov. (type strain CF-1111T=CGMCC 1.16786T=GDMCC 1.1587T=JCM 33490T), respectively.

scholarly journals Puniceibacterium antarcticum gen. nov., sp. nov., isolated from seawater

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1566-1572 ◽  
Author(s):  
Chang Liu ◽  
Xi-Ying Zhang ◽  
Hai-Nan Su ◽  
Ming-Yang Zhou ◽  
Bo Chen ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Novel Species ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-reaction-negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain SM1211T, was isolated from Antarctic seawater. The isolate grew at 4–35 °C and with 0–10 % (w/v) NaCl. It could produce bacteriochlorophyll a, but did not reduce nitrate to nitrite or hydrolyse DNA. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1211T constituted a distinct phylogenetic line within the family Rhodobacteraceae and was closely related to species in the genera Litorimicrobium , Leisingera , Seohaeicola and Phaeobacter with 95.1–96.0 % similarities. The predominant cellular fatty acid was C18 : 1ω7c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and two unidentified phospholipids. The genomic DNA G+C content of strain SM1211T was 60.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain SM1211T is considered to represent a novel species in a new genus within the family Rhodobacteraceae , for which the name Puniceibacterium antarcticum gen. nov., sp. nov. is proposed. The type strain of Puniceibacterium antarcticum is SM1211T ( = CCTCC AB 2013147T = KACC 16875T).

scholarly journals Halioglobus japonicus gen. nov., sp. nov. and Halioglobus pacificus sp. nov., members of the class Gammaproteobacteria isolated from seawater

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1784-1789 ◽  
Author(s):  
Sanghwa Park ◽  
Susumu Yoshizawa ◽  
Kentaro Inomata ◽  
Kazuhiro Kogure ◽  
Akira Yokota
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Optimum Growth ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
The North ◽  
North Western

Two coccoid, non-motile bacteria were isolated from seawater in the north-western Pacific Ocean near Japan. The two strains, designated S1-36T and S1-72T, were Gram-negative, obligately aerobic, heterotrophic and catalase-negative. They were able to reduce nitrate to nitrogen. Both strains required NaCl for growth, with optimum growth in 2 % NaCl, and grew at 15–30 °C, with optimum growth at 20–25 °C. Genomic DNA G+C contents of strains S1-36T and S1-72T were 59.6 and 59.4 mol%, respectively. The predominant isoprenoid quinone was Q-8 and major cellular fatty acids were C16 : 1ω7c, C18 : 1ω7c and C17 : 1ω8c. Analyses of 16S rRNA gene sequences revealed that strains S1-36T and S1-72T were related to each other (96.1 % sequence similarity) and both strains showed 92.3–94.7 % sequence similarity with members of the genus Haliea . On the basis of phenotypic and phylogenetic features, strains S1-36T and S1-72T should be classified as representatives of two novel species in a new genus, Halioglobus gen. nov., within the class Gammaproteobacteria . The names proposed are Halioglobus japonicus sp. nov., the type species of the genus, with S1-36T ( = NBRC 107739T = KCTC 23429T) as type strain, and Halioglobus pacificus sp. nov., with S1-72T ( = NBRC 107742T = KCTC 23430T) as type strain.

scholarly journals Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3473-3477 ◽  
Author(s):  
Qian-Qian Liu ◽  
Xiao-Li Li ◽  
Alejandro P. Rooney ◽  
Zong-Jun Du ◽  
Guan-Jun Chen
Keyword(s):  
New Genus ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Qinghai Province ◽  
Link Type ◽  
The Family

A novel Gram-stain-negative, facultatively anaerobic, catalase- and oxidase-positive, non-motile and pink-pigmented bacterium, designated G22T, was isolated from Gahai, a saltwater lake in Qinghai province, China. Optimal growth occurred at 33–35 °C, pH 7.0–7.5, and in the presence of 2–4 % (w/v) NaCl. The DNA G+C content was 40.0 mol%. The major polar lipids were phosphatidylethanolamine and three unknown lipids. The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH, and MK-7 was the main respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain G22T fell within the class Bacteroidia . Its closest phylogenetic neighbour was the recently described species Draconibacterium orientale , the sole member of the family Draconibacteriaceae , with merely 90.04 % sequence similarity. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence observed, a novel species in a new genus, Tangfeifania diversioriginum gen. nov., sp. nov., is proposed within the family Draconibacteriaceae . The type strain is G22T ( = CICC 10587T = DSM 27063T).

scholarly journals Flavivirga jejuensis gen. nov., sp. nov., and Flavivirga amylovorans sp. nov., new members of the family Flavobacteriaceae isolated from seawater, and emended descriptions of the genera Psychroserpens and Lacinutrix

2012 ◽  
Vol 62 (Pt_5) ◽  
pp. 1061-1068 ◽  
Author(s):  
Hana Yi ◽  
Jang-Cheon Cho ◽  
Jongsik Chun
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
Gliding Motility ◽  
Phenotypic Traits ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
The Family

Two yellow, rod-shaped, Gram-reaction-negative, strictly aerobic bacterial strains, designated JC2682T and JC2681T, were isolated from a seawater sample of Jeju Island, Korea. The isolates required sea salts for growth and showed gliding motility. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains JC2682T and JC2681T shared 96.9 % similarity and formed a robust monophyletic group within the radiation encompassing the family Flavobacteriaceae . This distinct clade, which had varying branch positions depending on the tree-making algorithm used, represents a new genus within the family Flavobacteriaceae . The predominant isoprenoid quinone (menaquinone 6, MK-6) and DNA G+C content (27–33 mol%) were consistent with its assignment to the family Flavobacteriaceae , but the polar lipid profile, fatty acid composition and overall phenotypic traits demonstrated that the test strains were not closely affiliated to any recognized genera. Based on data from the present polyphasic taxonomic study, strains JC2682T and JC2681T are considered to represent two novel species of a new genus in the family Flavobacteriaceae , for which the names Flavivirga jejuensis gen. nov., sp. nov. and Flavivirga amylovorans sp. nov. are proposed, respectively. The type strain of Flavivirga jejuensis is JC2682T ( = KACC 14158T  = JCM 17113T). The type strain of Flavivirga amylovorans is JC2681T ( = KACC 14157T  = JCM 17112T). Amended descriptions of the genera Psychroserpens and Lacinutrix are provided.

scholarly journals Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1760-1765 ◽  
Author(s):  
Martha Helena Ramírez-Bahena ◽  
Carmen Tejedor ◽  
Isidro Martín ◽  
Encarna Velázquez ◽  
Alvaro Peix
Keyword(s):  
Type Species ◽  
New Genus ◽  
Culture Media ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
The 16S Rrna Gene

A bacterial strain designated M1MS02T was isolated from a surface-sterilized nodule of Medicago sativa in Zamora (Spain). The 16S rRNA gene sequence of this strain showed 96.5 and 96.2 % similarity, respectively, with respect to Gluconacetobacter liquefaciens IFO 12388T and Granulibacter bethesdensis CGDNIH1T from the family Acetobacteraceae . The novel isolate was a Gram-stain-negative, non-sporulating, aerobic coccoid to rod-shaped bacterium that was motile by a subpolar flagellum. The major fatty acid was C18 : 1ω7c and the major ubiquinone was Q-10. The lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, three aminolipids, four glycolipids, two phospholipids and one lipid. Strain M1MS02T was catalase-positive and oxidase- and urease-negative. Acetate and lactate were not oxidized. Acetic acid was produced from ethanol in culture media supplemented with 2 % CaCO3. Ammonium sulphate was assimilated in glucose medium. The strain produced dihydroxyacetone from glycerol. Phylogenetic and phenotypic analyses commonly used to differentiate genera within the family Acetobacteraceae showed that strain M1MS02T should be classified as representing a novel species of a new genus within this family, for which the name Endobacter medicaginis gen. nov., sp. nov. is proposed. The type strain of the type species is M1MS02T ( = LMG 26838T = CECT 8088T). To our knowledge, this is the first report of a member of the Acetobacteraceae occurring as a legume nodule endophyte.

scholarly journals Enteractinococcus coprophilus gen. nov., sp. nov., of the family Micrococcaceae , isolated from Panthera tigris amoyensis faeces, and transfer of Yaniella fodinae Dhanjal et al. 2011 to the genus Enteractinococcus as Enteractinococcus fodinae comb. nov.

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2710-2716 ◽  
Author(s):  
Yan-Ru Cao ◽  
Yi Jiang ◽  
Rong-Xian Jin ◽  
Li Han ◽  
Wen-Xiang He ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
New Genus ◽  
Sequence Data ◽  
Rrna Gene ◽  
Panthera Tigris ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Phosphatidylinositol Mannosides

A novel actinobacterium, designated strain YIM 100590T, was isolated from Panthera tigris amoyensis faeces collected from Yunnan Wild Animal Park in Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 100590T is a member of the family Micrococcaceae . Cells were coccoid to oval (0.7–1.5 µm in diameter) occurring singly or in clusters. Growth was observed at 10–37 °C (optimum 28 °C) and at pH 7.0–11.0 (optimum pH 8.0). The major fatty acids were iso-C15 : 0 (32.22 %), anteiso-C15 : 0 (31.64 %) and iso-C16 : 0 (17.38 %). The peptidoglycan was of A4α type (l-Lys–Gly–l-Glu). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, dimannosyl diacylglycerol, an unknown glycolipid and two unknown phospholipids. The quinone system comprised menaquinones MK-7 (91.9 %) and MK-8 (8.3 %). The DNA G+C content of strain YIM 100590T was 56.2 mol%. Chemotaxonomic data indicated that the strain belongs to the family Micrococcaceae . On the basis of morphological and chemotaxonomic data and phylogenetic analysis, strain YIM 100590T is considered to represent a novel species of a new genus within the family Micrococcaceae , for which the name Enteractinococcus coprophilus gen. nov., sp. nov. is proposed. The type strain of Enteractinococcus coprophilus is YIM 100590T ( = DSM 24083T = JCM 17352T). Yaniella fodinae DSM 22966T was transferred to the new genus as Enteractinococcus fodinae comb. nov. (type strain G5T = DSM 22966T = JCM 17931T = MTCC 9846T).

scholarly journals Huaishuia halophila gen. nov., sp. nov., isolated from coastal seawater

2012 ◽  
Vol 62 (1) ◽  
pp. 223-228 ◽  
Author(s):  
Hong Wang ◽  
Xiuming Zhang ◽  
Shulin Yan ◽  
Zizhong Qi ◽  
Yong Yu ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Novel Species ◽  
Coastal Region ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Cellular Fatty Acids ◽  
Distinct Evolutionary Lineage ◽  
Motile Bacterium

A novel Gram-negative, non-motile bacterium, designated ZXM137T, was isolated from seawater collected from a coastal region of Qingdao, China, during a massive green algae (Enteromorpha prolifera) bloom. Strain ZXM137T was strictly aerobic and did not accumulate poly-β-hydroxybutyrate. Growth occurred with 0.5–11.0 % (w/v) NaCl, at pH 6–9 (optimum of pH 7) and at 4–45 °C (optimum at 28 °C). It contained Q-10 as the predominant ubiquinone and the major polar lipids were phosphatidylglycerol, phospholipids, and an unidentified aminolipid and lipid. The major cellular fatty acids of strain ZXM137T were C18 : 1ω7c, C18 : 1ω6c and 11-methyl C18 : 1ω7c. The DNA G+C content was 60.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZXM137T formed a distinct evolutionary lineage within the Roseobacter group in the class Alphaproteobacteria. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain ZXM137T represents a novel species in a new genus, for which the name Huaishuia halophila gen. nov., sp. nov. is proposed; the type strain is ZXM137T ( = CGMCC 1.8891T = LMG 24854T).

Extensimonas vulgaris gen. nov., sp. nov., a member of the family Comamonadaceae

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2062-2068 ◽  
Author(s):  
Wei-Yan Zhang ◽  
Ming-Xu Fang ◽  
Wen-Wu Zhang ◽  
Chuan Xiao ◽  
Xin-Qi Zhang ◽  
...  
Keyword(s):  
Industrial Wastewater ◽  
New Genus ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Sequences Alignment

A novel strain, named S4T, was obtained from industrial wastewater in Xiaoshan, Zhejiang Province, China. Cells were Gram-negative, neutrophilic and non-spore-forming and moved by means of a polar flagellum. Normal cells were 0.8–0.9×1.3–1.9 µm and the cells elongated to 10–25 µm when cultivated at high temperatures. Strain S4T grew at 15–50 °C (optimum at 48 °C), pH 5.5–8.5 (optimum 7.0–7.5) and 0–2 % (optimum 0.5 %) (w/v) NaCl. Ubiquinone-8 was the predominant respiratory quinone. C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C17 : 0 cyclo were the major cellular fatty acids. The major 3-OH fatty acid was C10 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminoglycolipid. The genomic DNA G+C content was 68.8 mol%. Based on 16S rRNA gene sequences alignment, the most closely related strains were members of the genera Comamonas (94.6–95.6 % similarities), Giesbergeria (94.9–95.6 %), Acidovorax (94.8–95.4 %), Brachymonas (94.1–95.2 %) and Macromonas (95.1 %). Phylogenetic analysis showed the closest relatives of strain S4T were members of the genus Macromonas . Based on phenotypic and phylogenetic characteristics, we suggest that strain S4T represents a novel species of a new genus of the family Comamonadaceae , for which the name Extensimonas vulgaris gen. nov., sp. nov. is proposed. The type strain of Extensimonas vulgaris is S4T ( = CGMCC 1.10977T = JCM 17803T).

Reclassification of [Glaciecola] lipolytica and [ Aestuariibacter] litoralis in Aliiglaciecola gen. nov., as Aliiglaciecola lipolytica comb. nov. and Aliiglaciecola litoralis comb. nov., respectively

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 2859-2864 ◽  
Author(s):  
Wen Dar Jean ◽  
Cheng Yu Hsu ◽  
Ssu-Po Huang ◽  
Jwo-Sheng Chen ◽  
Saulwood Lin ◽  
...  
Keyword(s):  
Nitrate Reduction ◽  
Type Strain ◽  
Type Species ◽  
New Genus ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Major Isoprenoid Quinone

Following phylogenetic analysis based on 16S rRNA gene sequences, together with DNA G+C contents and differential chemotaxonomic and physiological characteristics, a new genus with the name Aliiglaciecola gen. nov. is proposed to more appropriately accommodate two recognized species of the genera Glaciecola and Aestuariibacter . Accordingly, [Glaciecola] lipolytica and [ Aestuariibacter] litoralis should be reassigned to the novel genus as Aliiglaciecola lipolytica comb. nov. (type strain, E3T = JCM 15139T = CGMCC 1.7001T) and Aliiglaciecola litoralis comb. nov. (type strain, KMM 3894T = JCM 15896T = NRIC 0754T), respectively. Aliiglaciecola lipolytica is proposed as the type species of this new genus. Physiologically, the combined characteristics of positive reactions for nitrate reduction and growth at 4 °C and 36 °C distinguish the new genus from the genera Aestuariibacter and Glaciecola by one to three traits. Moreover, the new genus is also distinguished from the genus Glaciecola by the fatty acid profile and distinguished from the genus Aestuariibacter by the differences of major isoprenoid quinone (MK-7 vs Q-8) and DNA G+C content (40.8–43.0 mol% vs 48.0–54.0 mol%).

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