Lachancea lanzarotensis sp. nov., an ascomycetous yeast isolated from grapes and wine fermentation in Lanzarote, Canary Islands

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 358-363 ◽  
Author(s):  
Sara S. González ◽  
Julia Alcoba-Flórez ◽  
Federico Laich
Keyword(s):  
Canary Islands ◽  
Yeast Species ◽  
Lsu Rdna ◽  
Ascomycetous Yeast ◽  
Grape Berries ◽  
Physiological Characterization ◽  
D2 Domain ◽  
Ascosporogenous Yeast ◽  
Bioclimatic Conditions ◽  
Red Grape

During the characterization of the microbiota biodiversity associated with grapes and wineries in different bioclimatic conditions of the Canary Islands (Spain), a novel yeast species was isolated from Lanzarote, the driest wine-producing region of the archipelago. Seven strains isolated from grapes, microvinifications and wineries are described. Sequence analysis of the D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions revealed that the isolates were phylogenetically a member of the genus Lachancea and are closely related to Lachancea meyersii NRRL Y-27269T and Lachancea nothofagi NRRL Y-48670T. On the basis of morphological, biochemical and physiological characterization and phylogenetic analysis, a novel ascosporogenous yeast species, Lachancea lanzarotensis sp. nov., is proposed. The type strain is L2C-15T ( = CBS 12615T = CECT 13066T) which was isolated from grape berries of Vitis vinifera L. cv. Listán Negro red grape variety in Tinajo, Lanzarote. The MycoBank no. is MB 801390.

scholarly journals Lachancea quebecensis sp. nov., a yeast species consistently isolated from tree bark in the Canadian province of Québec

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3392-3399 ◽  
Author(s):  
Kelle C. Freel ◽  
Guillaume Charron ◽  
Jean-Baptiste Leducq ◽  
Christian R. Landry ◽  
Joseph Schacherer
Keyword(s):  
Type Strain ◽  
Apple Tree ◽  
Yeast Species ◽  
Its Region ◽  
Tree Bark ◽  
Lsu Rdna ◽  
Canadian Province ◽  
D2 Domain ◽  
Ascosporogenous Yeast ◽  
Lachancea Thermotolerans

A thorough sampling of maple, oak, birch, and apple tree bark in North America yielded a set of isolates that represent a yeast species not yet formally described. The strains obtained were all isolated from the Canadian province of Québec. These four isolates have identical electrophoretic karyotypes, distinct from other species of the genus Lachancea, and are most closely related to the formally recognized species Lachancea thermotolerans according to the D1/D2 domain of the LSU rDNA gene and 5.8S–ITS region. Previous studies revealed the existence of a population of strains closely related to L. thermotolerans, with unique D1/D2 sequences and the ability to grow on melibiose, which is also true for these isolates. The sequences obtained here (for the D1/D2, and 5.8S–ITS region) are identical among the four strains, and in a phylogenetic analysis of the D1/D2 region, the strains form a distinct clade with the previously described population closely related to L. thermotolerans, composed of isolates from Japan, as well as from the provinces of Ontario and Québec in Canada. On the basis of select physiological and phylogenetic characteristics, a novel ascosporogenous yeast species, Lachancea quebecensis sp. nov., is proposed. The type strain LL11_022T ( = CBS 14138T = CLIB 1763T = UCDFST 15-106T) was isolated from maple tree bark in the Station Duchesnay, QC region of Québec, Canada. The MycoBank number is MB811749.

Rhodotorula portillonensis sp. nov., a basidiomycetous yeast isolated from Antarctic shallow-water marine sediment

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3884-3891 ◽  
Author(s):  
Federico Laich ◽  
Inmaculada Vaca ◽  
Renato Chávez
Keyword(s):  
Shallow Water ◽  
Marine Sediment ◽  
Yeast Species ◽  
Phylogenetic Analyses ◽  
Basidiomycetous Yeast ◽  
Lsu Rdna ◽  
King George Island ◽  
Physiological Characterization ◽  
D2 Domain

During the characterization of the mycobiota associated with shallow-water marine environments from Antarctic sea, a novel pink yeast species was isolated. Sequence analysis of the D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions revealed that the isolated yeast was closely related to Rhodotorula pallida CBS 320T and Rhodotorula benthica CBS 9124T. On the basis of morphological, biochemical and physiological characterization and phylogenetic analyses, a novel basidiomycetous yeast species, Rhodotorula portillonensis sp. nov., is proposed. The type strain is Pi2T ( = CBS 12733T  = CECT 13081T) which was isolated from shallow-water marine sediment in Fildes Bay, King George Island, Antarctica.

Tetrapisispora namnaonensis sp. nov., a novel ascomycetous yeast species isolated from forest soil of Nam Nao National Park, Thailand

2005 ◽  
Vol 55 (4) ◽  
pp. 1735-1738 ◽  
Author(s):  
Tawatchai Sumpradit ◽  
Savitree Limtong ◽  
Wichien Yongmanitchai ◽  
Hiroko Kawasaki ◽  
Tatsuji Seki
Keyword(s):  
National Park ◽  
Base Composition ◽  
Nuclear Dna ◽  
Yeast Species ◽  
Large Subunit ◽  
Lsu Rdna ◽  
Dna Base Composition ◽  
Ascomycetous Yeast ◽  
Dna Base ◽  
D2 Domain

Twenty-one strains of a novel ascomycetous yeast species were isolated from soil collected in three kinds of natural forest, namely a dry dipterocarp forest, a mixed deciduous forest and a pine forest, in Nam Nao National Park, Phetchabun province, Thailand. The strains formed asci containing one to four ovoid to reniform ascospores, assimilated glucose, galactose and glycerol, fermented glucose and galactose vigorously and contained ubiquinone Q-6, indicating that they belonged to the genus Tetrapisispora. A comparative analysis of the small subunit rDNA (SSU rDNA) and the D1/D2 domain of the large subunit rDNA (LSU rDNA) of all available sequences for ascomycetous yeasts confirmed that the strains were phylogenetically related to the genus Tetrapisispora. All strains had identical nucleotide sequences in the D1/D2 domain of the LSU rDNA and differed from the nearest species, Tetrapisispora arboricola IFO 10925T, by 6·4 % nucleotide substitutions. The strains differed from Tetrapisispora arboricola by the ability to assimilate d-gluconic acid, the inability to grow on 50 % glucose medium, the nuclear DNA base composition and deliquescent asci. The strains were differentiated from the other four species of Tetrapisispora on the basis of trehalose assimilation, the ability to grow on 50 % glucose or 10 % NaCl plus 5 % glucose, vitamin requirement, the nuclear DNA base composition and the type of ascus. Based on the characteristics mentioned above, the strains are recognized as a single novel species of the genus Tetrapisispora and the name Tetrapisispora namnaonensis sp. nov. is proposed. The type strain is TN1-01T (=TISTR 5828T=JCM 12664T=CBS 10093T).

Reaction of an ascomycetous yeast with diazonium blue B

1982 ◽  
Vol 28 (10) ◽  
pp. 1194-1195 ◽  
Author(s):  
Marc-André Lachance
Keyword(s):  
Yeast Species ◽  
Ascomycetous Yeast ◽  
Ascosporogenous Yeast

The new ascosporogenous yeast species Sporopachydermia quercuum has been found to react positively with the diazonium blue B reagent, a feature normally associated exclusively with yeasts of basidiomycetous affinity.

Naumovozyma baii sp. nov., an ascomycetous yeast species isolated from rotten wood in a tropical forest

2012 ◽  
Vol 62 (Pt_12) ◽  
pp. 3095-3098 ◽  
Author(s):  
Wan-Qiu Liu ◽  
Pei-Jie Han ◽  
Jun-Zhi Qiu ◽  
Qi-Ming Wang
Keyword(s):  
Gene Sequence ◽  
Yeast Species ◽  
Morphological Characteristics ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
26S Rrna

Two strains isolated from rotten wood were included in the Saccharomyces group based on morphological characteristics. However, rRNA gene sequence analyses (including the 18S rRNA gene, 26S rRNA gene D1/D2 domain and internal transcribed spacer region) indicated that these two strains represent a novel species of Naumovozyma, for which the name Naumovozyma baii sp. nov. is proposed (type strain: BW 22T = CGMCC 2.04520T = CBS 12642T). The MycoBank number of the new species is MB800484.

Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 393-397 ◽  
Author(s):  
Stephen A. James ◽  
Enrique Javier Carvajal Barriga ◽  
Patricia Portero Barahona ◽  
Kathryn Cross ◽  
Christopher J. Bond ◽  
...  
Keyword(s):  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
Lsu Rrna Gene

In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004T) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represent a novel yeast species based on the sequences of their D1/D2 domain of the large-subunit (LSU) rRNA gene and their physiological characteristics. Phylogenetic analysis based on LSU D1/D2 sequences revealed this novel species to belong to the Metschnikowia clade and to be most closely related to Candida suratensis, a species recently discovered in a mangrove forest in Thailand. The species name of Candida ecuadorensis sp. nov. is proposed to accommodate these strains, with strain CLQCA 20-004T ( = CBS 12653T = NCYC 3782T) designated as the type strain.

scholarly journals Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov., novel ascomycetous yeast species

2005 ◽  
Vol 55 (5) ◽  
pp. 2219-2224 ◽  
Author(s):  
Zuo-Wei Wu ◽  
Feng-Yan Bai
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Culture Collection ◽  
Electrophoretic Karyotype ◽  
Ascomycetous Yeast ◽  
Centraalbureau Voor ◽  
Domain Sequence ◽  
D2 Domain ◽  
Beijing China

The unidentified strains AS 2.0706T, preserved in the China General Microbiological Culture Collection Center (CGMCC), Academia Sinica, Beijing, China, and CBS 6904T, preserved in the Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands, were shown to represent two novel ascomycetous yeast species of the genus Kazachstania by 18S rDNA, internal transcribed spacer (ITS) region (including 5·8S rDNA) and 26S rDNA D1/D2 domain sequence analysis and electrophoretic karyotype comparison. The names Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov. are proposed for strains AS 2.0706T and CBS 6904T, respectively. Phylogenetically, the two novel species are closely related to Kazachstania aerobia, Kazachstania servazzii and Kazachstania unispora.

Kodamaea kakaduensisandCandida tolerans, two new ascomycetous yeast species from AustralianHibiscusflowers

1999 ◽  
Vol 45 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Marc-André Lachance ◽  
Jane M Bowles ◽  
William T Starmer ◽  
J Stuart F. Barker
Keyword(s):  
Ribosomal Dna ◽  
Yeast Species ◽  
Large Subunit ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
Carbon And Nitrogen ◽  
D2 Domain ◽  
New Yeast ◽  
Type Strains ◽  
Candida Mogii

Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs from others by the assimilation of trehalose, melezitose, and xylitol, and is reproductively isolated. The cells of Candida tolerans are small and a pseudomycelium is formed. The carbon and nitrogen assimilation pattern is reminiscent of that of Zygosaccharomyces rouxii but the two are not closely related. Sequences of the D1/D2 domain of large subunit ribosomal DNA confirm the membership of K. kakaduensis in the genus Kodamaea and indicate that C. tolerans belongs to the Clavispora-Metschnikowia clade, with a moderate relatedness to Candida mogii. The type strains are: K. kakaduensis, UWO(PS)98-119.2 (h+, holotype, CBS 8611) and UWO(PS)98-117.1 (h-, isotype, CBS 8612); and C. tolerans, UWO(PS)98-115.5 (CBS 8613).Key words: Kodamaea, Candida, new yeast species, ribosomal DNA, whole-cell PCR.

scholarly journals Teunomyces basidiocarpi sp. nov. and Teunomyces luguensis sp. nov., two ascomycetous yeast species related to the Candida kruisii clade isolated in Taiwan

2020 ◽  
Vol 70 (3) ◽  
pp. 2103-2107
Author(s):  
Chin-Feng Chang ◽  
Yi-Ru Liu ◽  
Ching-Fu Lee
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Domain Sequence ◽  
D2 Domain ◽  
Lsu Rrna Gene ◽  
Lsu Rrna ◽  
Physiological Tests ◽  
Type Strains

Four strains of anamorphic yeasts isolated from the fruiting bodies of mushrooms collected in Taiwan were found to represent two novel yeast species belonging to the genus Teunomyces, which was formally known as the Candida kruisii clade. Strains NY13M09T and NY14M14 were related to the type strains of Teunomyces panamensis, T. pallodes, T. tritomae and T. lycoperdinae, and strains GG4M07T and GG6M14 were related to T. kruisii NRRL Y-17087T and T. cretensis NRRL Y-27777T. However, strains NY13M09T and NY14M14 differed from their closest phylogenetic neighbours by 2.9–3.7 % in the D1/D2 domain sequence of the LSU rRNA gene and by 6.6–13.7 % in the internal transcribed spacer (ITS); GG4M07T and GG6M14 differed from their closest known species by 2.4 % in the D1/D2 domain sequence of the LSU rRNA gene and by 8.7–10.0 % in the ITS. Meanwhile, these strains were also clearly distinguished from their closest relatives based on the results of physiological tests. Based on the characteristics described above, the strains could be regarded as representing two novel species of the genus Teunomyces, for which the names Teunomyces basidiocarpi sp. nov. and Teunomyces luguensis sp. nov. are proposed. The holotypes are Teunomyces basidiocarpi BCRC 23475T and Teunomyces luguensis BCRC 23476T.

scholarly journals Clavispora santaluciae f.a., sp. nov., a novel ascomycetous yeast species isolated from grapes

2020 ◽  
Vol 70 (12) ◽  
pp. 6307-6312
Author(s):  
João Drumonde-Neves ◽  
Neža Čadež ◽  
Yazmid Reyes-Domínguez ◽  
Andreas Gallmetzer ◽  
Dorit Schuller# ◽  
...  
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Drosophila Suzukii ◽  
Nucleotide Substitutions ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
Lsu Rrna Gene ◽  
Additional Strain

During a study of yeast diversity in Azorean vineyards, four strains were isolated which were found to represent a novel yeast species based on the sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and of the D1/D2 domain of the large subunit (LSU) rRNA gene, together with their physiological characteristics. An additional strain isolated from Drosophila suzukii in Italy had identical D1/D2 sequences and very similar ITS regions (five nucleotide substitutions) to the Azorean strains. Phylogenetic analysis using sequences of the ITS region and D1/D2 domain showed that the five strains are closely related to Clavispora lusitaniae, although with 56 nucleotide differences in the D2 domain. Intraspecies variation revealed between two and five nucleotide differences, considering the five strains of Clavispora santaluciae. Some phenotypic discrepancies support the separation of the new species from their closely related ones, such as the inability to grow at temperatures above 35 °C, to produce acetic acid and the capacity to assimilate starch. Neither conjugations nor ascospore formation were observed in any of the strains. The name Clavispora santaluciae f.a., sp. nov., is proposed to accommodate the above noted five strains (holotype, CBS 16465T; MycoBank no., MB 835794).

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