scholarly journals Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1461-1467 ◽  
Author(s):  
Learn-Han Lee ◽  
Nurullhudda Zainal ◽  
Adzzie-Shazleen Azman ◽  
Nurul-Syakima Ab Mutalib ◽  
Kui Hong ◽  
...  
Keyword(s):  
Cell Wall ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel actinobacterial strain, designated MUSC 201T, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201T represented a novel lineage within the class Actinobacteria . Strain MUSC 201T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9–95.1 %), Aeromicrobium (92.7–94.6 %), Marmoricola (92.5–93.1 %) and Kribbella (91.5–92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained ll-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3γ. The peptidoglycan cell wall contained ll-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5 : 0.9 : 1.0 : 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C18 : 1ω9c (30.8 %), C16 : 0 (24.1 %), and 10-methyl C18 : 0 (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae , a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201T ( = DSM 27763T = MCCC 1A00646T = NBRC 109973T).

scholarly journals Marininema mesophilum gen. nov., sp. nov., a thermoactinomycete isolated from deep sea sediment, and emended description of the family Thermoactinomycetaceae

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1383-1388 ◽  
Author(s):  
Jie Li ◽  
Guang-Tao Zhang ◽  
Jian Yang ◽  
Xin-Peng Tian ◽  
Fa-Zuo Wang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel filamentous bacterium, strain SCSIO 10219T, was isolated from a sediment sample collected from the South China Sea (113° 3.752′ E 18° 1.722′ N) at a depth of 2105 m. Growth was observed at 25–35 °C (optimum 30 °C) and pH 5.0–8.0 (optimum pH 6.0–7.0). The organism formed yellow–white colonies with radial wrinkles. Aerial mycelium was not produced on any of the growth media tested. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain SCSIO 10219T belongs to the family Thermoactinomycetaceae . The strain contained ll-diaminopimelic acid in the cell wall. The predominant menaquinone was MK-7. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and five unknown phospholipids. Major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The DNA G+C content was 46.5 mol%. On the basis of chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain represents a novel species in a new genus, Marininema mesophilum gen. nov., sp. nov., in the family Thermoactinomycetaceae . The type strain of the type species is SCSIO 10219T ( = CCTCC AA 2011006T = DSM 45610T). In addition, we propose that the description of the family Thermoactinomycetaceae should be further emended based on the present study.

scholarly journals Terrabacter koreensis sp. nov., isolated from soil of a flowerbed

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3335-3340 ◽  
Author(s):  
KyungHwa Won ◽  
MooChang Kook ◽  
Tae-Hoo Yi
Keyword(s):  
Cell Wall ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A Gram-staining-positive, non-spore-forming, rod-shaped, non-motile and aerobic bacterium, strain THG-e54T, was isolated from soil of a flowerbed in Bucheon, South Korea. The taxonomic position of this bacterium was determined in an investigation based on a polyphasic approach. 16S rRNA gene sequence analysis revealed a clear affiliation of strain THG-e54T with the genus Terrabacter . Strain THG-e54T showed the closest phylogenetic relationship with Terrabacter tumescens DSM 20308T with a 16S rRNA gene sequence similarity of 98.6 %. The G+C content of the genomic DNA was 71.0 mol%. The major cellular fatty acids of strain THG-e54T were iso-C15 : 0 and iso-C16 : 0, and the predominant menaquinone was menaquinone MK-8(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified amino-containing phosphoglycolipid, unidentified phospholipids, an unidentified aminolipid and an unidentified aminophospholipid. The cell-wall peptidoglycan type was ll-diaminopimelic acid and the cell-wall sugars were glucose and ribose. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 35.2 %) between strain THG-e54T and its five closest relatives. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-e54T could be clearly distinguished from closely related recognized members of the genus Terrabacter . Therefore the results of this study indicated the existence of a novel species of the genus Terrabacter , for which we propose the name Terrabacter koreensis sp. nov., with strain THG-e54T ( = KACC 17589T = JCM 19342T) as the type strain.

scholarly journals Oceanobacillus luteolus sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1495-1500 ◽  
Author(s):  
Min Wu ◽  
Guiqin Yang ◽  
Zhen Yu ◽  
Li Zhuang ◽  
Yingqiang Jin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two Gram-stain-positive, rod-shaped and endospore-forming bacteria, designated WM-1T and WM-4, were isolated from a paddy soil and a forest soil, respectively, in South China. Comparative 16S rRNA gene sequence analyses showed that both strains were members of the genus Oceanobacillus and most closely related to Oceanobacillus chironomi LMG 23627T with pairwise sequence similarity of 96.0 %. The isolates contained menaquinone-7 (MK-7) as the respiratory quinone and anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0 as the major fatty acids (>10 %). Polar lipids consisted of a predominance of diphosphatidylglycerol and moderate to minor amounts of phosphatidylglycerol and phosphatidylinositol. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The DNA G+C content was 38.6–39.2 mol%. The 16S rRNA gene sequence of strain WM-1T displayed 99.7 % similarity to that of strain WM-4, and DNA–DNA hybridization between the two strains showed a relatedness value of 91 %. Based on the results of this polyphasic study, strains WM-1T and WM-4 represent a novel species in the genus Oceanobacillus , for which the name Oceanobacillus luteolus sp. nov. is proposed. The type strain is WM-1T ( = KCTC 33119T = CGMCC 1.12406T).

scholarly journals Thermasporomyces composti gen. nov., sp. nov., a thermophilic actinomycete isolated from compost

2011 ◽  
Vol 61 (1) ◽  
pp. 86-90 ◽  
Author(s):  
Shuhei Yabe ◽  
Yoshifumi Aiba ◽  
Yasuteru Sakai ◽  
Masaru Hazaka ◽  
Akira Yokota
Keyword(s):  
Cell Wall ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Optimum Growth ◽  
Rrna Gene Sequence ◽  
The Family

A thermophilic, Gram-positive bacterium that formed a branched vegetative mycelium was isolated from compost. The strain, designated I3T, grew at temperatures between 35 and 62 °C, with optimum growth at 50–55 °C. No growth was observed below 29 °C or above 65 °C. The pH range for growth was 5.7–10.0, the pH for optimum growth was 7.0 and no growth was observed below pH 5.6 or above pH 10.8. The DNA G+C content of strain I3T was 69.2 mol%. The major fatty acids found were C15 : 0 iso (14.2 %), C15 : 0 anteiso (12.1 %), C17 : 0 iso (16.3 %) and C17 : 0 anteiso (21.7 %). The major menaquinones were MK-9(H4), MK-10(H4) and MK-11(H4). The cell wall contained glutamic acid, glycine, alanine and ll-diaminopimelic acid in a molar ratio of 1.0 : 3.9 : 0.6 : 0.5. The polar lipids consisted of ninhydrin-positive phosphoglycolipids, phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. The cell-wall sugars were rhamnose and arabinose. 16S rRNA gene sequence analysis assigned this actinomycete to the family Nocardioidaceae, but its 16S rRNA gene sequence shared no more than 95.5 % similarity with those of other members of the family. The chemotaxonomic and phenotypic characteristics of strain I3T differed in some respects from those of members of the genus Actinopolymorpha, the most closely related genus. Therefore, strain I3T represents a novel species in a new genus of the family Nocardioidaceae, for which the name Thermasporomyces composti gen. nov., sp. nov. is proposed. The type strain of the type species is I3T (=JCM 16421T=DSM 22891T).

scholarly journals Actinokineospora spheciospongiae sp. nov., isolated from the marine sponge Spheciospongia vagabunda

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 879-884 ◽  
Author(s):  
Peter Kämpfer ◽  
Stefanie P. Glaeser ◽  
Hans-Jürgen Busse ◽  
Usama Ramadan Abdelmohsen ◽  
Safwat Ahmed ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A Gram-staining-positive, aerobic organism, isolated from the Red Sea sponge Spheciospongia vagabunda was investigated to determine its taxonomic position. On the basis of results of 16S rRNA gene sequence analysis strain EG49T was most closely related to Actinokineospora cibodasensis and Actinokineospora baliensis (both 97.3 % similarity) and Actinokineospora diospyrosa and Actinokineospora auranticolor (both 97.0 % similarity). The 16S rRNA gene sequence similarity to all other species of the genus Actinokineospora was <97.0 %. The quinone system of strain EG49T contained the menaquinones MK-9(H4) (47 %), MK-9(H6) (27 %) and MK-9(H2) (15 %) in major amounts. Minor amounts of MK-7(H4) (2 %), MK-9(H0) (1 %), MK-9(H8) (3 %) and MK-10(H4) (3 %) were detected as well in addition to MK-8(H4), MK-8(H6), MK-10(H2) and MK-10(H6) (all <1 %). The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphosphatidylethanolamine were predominant. Phosphatidylinositol-mannoside, two unidentified phospholipids and two glycoglipids as well as one aminoglycolipid, one aminolipid and one unidentified lipid were found in addition. The fatty acid profile was composed of mainly iso-branched fatty acids: iso-C16 : 0, iso-C14 : 0, iso-C15 : 0 and iso-C16 : 1H. All these findings clearly supported the classification of the strain as representing a member of the genus Actinokineospora . In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain EG49T from the most closely related species of the genus Actinokineospora . Strain EG49T represents a novel species of the genus Actinokineospora , for which we propose the name Actinokineospora spheciospongiae sp. nov., with strain EG49T ( = DSM 45935T = CCM 8480T = LMG 27700T) as the type strain.

Nocardioides salsibiostraticola sp. nov., isolated from biofilm formed in coastal seawater

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3800-3806 ◽  
Author(s):  
Yirang Cho ◽  
Gwang Il Jang ◽  
Chung Yeon Hwang ◽  
Eun-Hye Kim ◽  
Byung Cheol Cho
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Coastal Seawater ◽  
Link Type

A Gram-staining-positive, non-motile, aerobic, non-spore-forming and short rod-shaped bacterial strain, PAMC 26527T, was isolated from biofilm formed in coastal seawater of the Norwegian Sea. Analysis of the 16S rRNA gene sequence of strain PAMC 26527T revealed a clear affiliation with the genus Nocardioides . Based on phylogenetic analysis, strain PAMC 26527T showed the closest phylogenetic relationship with Nocardioides caricicola YC6903T with 16S rRNA gene sequence similarity of 96.3 %. Strain PAMC 26527T grew in the presence of 0–5.0 % sea salts. The optimum temperature and pH for growth were 20 °C and pH 7.5. The major cellular fatty acids of strain PAMC 26527T were iso-C16 : 0, C17 : 1ω8c and C18 : 1ω9c and the major isoprenoid quinone was MK-8(H4). Cell-wall analysis showed that strain PAMC 26527T contained ll-diaminopimelic acid. The genomic DNA G+C content was 69.3 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain PAMC 26527T could be clearly distinguished from recognized members of the genus Nocardioides . Thus, strain PAMC 26527T should be classified as representing a novel species in the genus Nocardioides , for which the name Nocardioides salsibiostraticola sp. nov. is proposed. The type strain is PAMC 26527T ( = KCTC 29158T = JCM 18743T).

scholarly journals Streptomyces zagrosensis sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3434-3440 ◽  
Author(s):  
Fatemeh Mohammadipanah ◽  
Javad Hamedi ◽  
Cathrin Spröer ◽  
Manfred Rohde ◽  
María del Carmen Montero-Calasanz ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus Streptomyces . On ISP2 medium, strain HM 1154T produced a dark cream, branched substrate mycelium and Retinaculiaperti aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6–9 with 0–4 % (w/v) NaCl. Whole-cell hydrolysates contained ll-diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H4) and MK-9(H2) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C16 : 0 and anteiso-C15 : 0. Strain HM 1154T exhibited the highest 16S rRNA gene sequence similarities to Streptomyces coerulescens DSM 40146T (99.4 %), Streptomyces varsoviensis DSM 40346T (99.3 %), Streptomyces youssoufiensis DSM 41920T (99.2 %), Streptomyces abikoensis DSM 40831T (99.2 %), Streptomyces rimosus subsp. rimosus DSM 40260T (99.1 %), Streptomyces luteireticuli DSM 40509T (99.1 %), Streptomyces thioluteus DSM 40027T (99.1 %), Streptomyces blastmyceticus DSM 40029T (99.0 %) and Streptomyces hiroshimensis DSM 40037T (99.0 %). DNA–DNA hybridization studies showed relatedness values of 11.0–35.8 % with the closest related species. Based on these results, strain HM 1154T is considered to represent a novel species within the genus Streptomyces , for which the name Streptomyces zagrosensis sp. nov. is proposed. The type strain is HM 1154T ( = DSM 42018T = UTMC 1154T = CECT 8305T).

Lysinimonas soli gen. nov., sp. nov., isolated from soil, and reclassification of Leifsonia kribbensis Dastager et al. 2009 as Lysinimonas kribbensis sp. nov., comb. nov.

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1403-1410 ◽  
Author(s):  
Yun-Hee Jang ◽  
Soo-Jin Kim ◽  
Tomohiko Tamura ◽  
Moriyuki Hamada ◽  
Hang-Yeon Weon ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-stain-positive, non-motile rod, designated strain SGM3-12T, was isolated from paddy soil in Suwon, Republic of Korea. 16S rRNA gene sequence analysis revealed that the strain represented a novel member of the family Microbacteriaceae . The nearest phylogenetic neighbour was Leifsonia kribbensis MSL-13T (97.4 % 16S rRNA gene sequence similarity). Strain SGM3-12T and Leifsonia kribbensis MSL-13T formed a distinct cluster within the family Microbacteriaceae . Strain SGM3-12T contained MK-12(H2) and MK-11(H2) as the predominant menaquinones with moderate amounts of MK-12 and MK-11; anteiso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids (>10 % of total); and diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids as the polar lipids. The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid. On the basis of these results, strain SGM3-12T represents a novel species within a new genus, for which the name Lysinimonas soli gen. nov., sp. nov. is proposed (the type strain of the type species is SGM3-12T = KACC 13362T = NBRC 107106T). It is also proposed that Leifsonia kribbensis be transferred to this genus as Lysinimonas kribbensis comb. nov. (the type strain is MSL-13T = DSM 19272T = JCM 16015T = KACC 21108T = KCTC 19267T).

Melghirimyces profundicolus sp. nov., isolated from a deep-sea sediment

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4552-4556 ◽  
Author(s):  
Jie Li ◽  
Sheng Qin ◽  
Zhi-Qing You ◽  
Li-Juan Long ◽  
Xin-Peng Tian ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel filamentous bacterium, strain SCSIO 11153T, was isolated from a sediment sample collected from the Indian Ocean (80° 03.099′ E 01° 03.300′ N) at a depth of 4593 m. Good growth was observed at 50–55 °C and pH 7.0 with 3 % NaCl. It formed ivory–white colonies with radial wrinkles. Aerial mycelium was absent on the media tested. Phenotypic characteristics and 16S rRNA gene sequence analysis indicated that strain SCSIO 11153T belonged to the family Thermoactinomycetaceae . It exhibited 96.4 % and 96.2 % 16S rRNA gene sequence similarities to Melghirimyces algeriensis NariEXT and Melghirimyces thermohalophilus Nari11AT, respectively, while lower sequence similarity values (<95.4 %) were observed between strain SCSIO 11153T and other species of genera in the family Thermoactinomycetaceae . The menaquinone type was MK-7. Major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain SCSIO 11153T was 52.6 mol%. On the basis of the genotypic and phenotypic characteristics, it is proposed that strain SCSIO 11153T represents a novel species of the genus Melghirimyces with the name Melghirimyces profundicolus sp. nov. The type strain is SCSIO 11153T ( = DSM 45787T = CCTCC AA 2012007T = NBRC 109068T).

scholarly journals Rhodococcus aerolatus sp. nov., isolated from subarctic rainwater

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 465-471 ◽  
Author(s):  
C. Y. Hwang ◽  
I. Lee ◽  
Y. Cho ◽  
Y. M. Lee ◽  
K. Baek ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A Gram-stain-positive, rod-shaped and non-motile strain, designated PAMC 27367T, was isolated from rainwater collected on the Bering Sea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Rhodococcus . Phylogenetic analyses revealed that strain PAMC 27367T formed a robust clade with the type strains of Rhodococcus rhodnii , Rhodococcus aetherivorans and Rhodococcus ruber with 16S rRNA gene sequence similarities of 96.3 %, 95.8 % and 95.5 %, respectively. Cells of the strain grew optimally at 25 °C and at pH 6.5–7.0 in the presence of 0–2 % (w/v) sea salts. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and three unknown phospholipids. The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω8c and 10-methyl C17 : 0. Cell wall analysis showed that strain PAMC 27367T contained meso-diaminopimelic acid. The genomic DNA G+C content was 77.1 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Rhodococcus aerolatus sp. nov., with PAMC 27367T ( = KCTC 29240T = JCM 19485T) as the type strain.

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