scholarly journals Nonomuraea muscovyensis sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_7) ◽  
pp. 2467-2472 ◽  
Author(s):  
Fadime Ozdemir-Kocak ◽  
Kamil Isik ◽  
Aysel Veyisoglu ◽  
Demet Tatar ◽  
Nevzat Sahin
Keyword(s):  
Novel Species ◽  
Diaminopimelic Acid ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Content Type ◽  
Forest Park ◽  
Link Type ◽  
Physiological And Biochemical Characteristics ◽  
Actinomycete Strain ◽  
Reference Strains

A novel actinomycete, strain FMN03T, was isolated from a soil sample collected from Yuga Zapadnaya South-West Forest Park, Moscow, Russia. The isolate had chemical and morphological properties typical of members of the genus Nonomuraea and formed a distinct 16S rRNA gene subclade with the type strains Nonomuraea roseoviolacea subsp. carminata NBRC 15903T and Nonomuraea roseoviolacea subsp. roseoviolacea NBRC 14098T. The organism formed extensively branched substrate and aerial hyphae, which generated spiral chains of spores with smooth surfaces. The cell wall contained meso-diaminopimelic acid and the whole cell sugars were glucose, galactose and trace amounts of madurose, mannose and xylose. The polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, four unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C16 : 0 2-OH, C17 : 0 10-methyl, C17 : 1 cis9 and iso-C16 : 0. Analyses of its morphological, physiological and biochemical characteristics, together with DNA–DNA relatedness data, confirmed that strain FMN03T is a representative of a novel species of the genus Nonomuraea , which is distinct from closely related reference strains. Strain FMN03T ( = DSM 45913T = KCTC 29233T) is proposed as the type strain of a novel species, for which the name Nonomuraea muscovyensis sp. nov. is proposed.

scholarly journals Streptomyces barkulensis sp. nov., isolated from an estuarine lake

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1365-1372 ◽  
Author(s):  
Lopamudra Ray ◽  
Samir Ranjan Mishra ◽  
Ananta Narayan Panda ◽  
Gurdeep Rastogi ◽  
Ajit Kumar Pattanaik ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Threshold Value ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Phyletic Line ◽  
Actinomycete Strain ◽  
Reference Strains

The taxonomic position of a novel actinomycete, strain RC 1831T, isolated from the sediment of a fish dumping yard at Barkul village near Chilika Lake, Odisha, India, was determined by a polyphasic approach. Based on morphological and chemotaxonomic characteristics the isolate was determined to belong to the genus Streptomyces . The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1428 nt) with representative strains showed that the strain consistently falls into a distinct phyletic line together with Streptomyces glaucosporus DSM 41689T (98.22 % similarity) and a subclade consisting of Streptomyces atacamensis DSM 42065T (98.40 %), Streptomyces radiopugnans R97 DSM 41901T (98.27 %), Streptomyces fenghuangensis GIMN4.003T (98.33 %), Streptomyces nanhaiensis DSM 41926T (98.13 %), Streptomyces megasporus NBRC 14749T (97.37 %) and Streptomyces macrosporus NBRC 14748T (98.22 %). However, the levels of DNA–DNA relatedness between strain RC 1831T and phylogenetically related strains Streptomyces atacamensis DSM 42065T (28.75±3.25 %) and Streptomyces glaucosporus DSM 41689T (15±2.40 %) were significantly lower than the 70 % threshold value for delineation of genomic species. Furthermore, the isolate could be distinguished phenotypically on the basis of physiological, morphological and biochemical differences from its closest phylogenetic neighbours and other related reference strains. Strain RC 1831T is therefore considered to represent a novel species of the genus Streptomyces , for which the name Streptomyces barkulensis sp. nov. is proposed. The type strain is RC 1831T ( = JCM 18754T = DSM 42082T).

Dactylosporangium siamense sp. nov., isolated from soil

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4033-4038 ◽  
Author(s):  
Chitti Thawai ◽  
Chanwit Suriyachadkun
Keyword(s):  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Mycolic Acids ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Tropical Forest Soil ◽  
Actinomycete Strain

A novel actinomycete strain, designed MW4-36T, was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus Dactylosporangium . The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and meso-diaminopimelic acid including 3-hydroxy-meso-diaminopimelic acid; arabinose, glucose, rhamnose, ribose and xylose were found as whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; no phosphatidylcholine was found. The predominant menaquinones were MK-9(H8) and MK-9(H6). Mycolic acids were not detected. The predominant cellular fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 72.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium maewongense JCM 15933T (99.4 % similarity). These taxonomic data revealed that strain MW4-36T could be readily distinguished from its phylogenetically closest relative. On the basis of these phenotypic and genotypic data, strain MW4-36T is considered to represent a novel species, for which the name Dactylosporangium siamense sp. nov. is proposed. The type strain is MW4-36T ( = BCC 34901T = NBRC 106093T).

scholarly journals Micromonospora fluminis sp. nov., isolated from mountain river sediment

2020 ◽  
Vol 70 (12) ◽  
pp. 6428-6436 ◽  
Author(s):  
Miladis Isabel Camacho Pozo ◽  
Anneleen D. Wieme ◽  
Suyén Rodríguez Pérez ◽  
Gabriel Llauradó Maury ◽  
Charlotte Peeters ◽  
...  
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Santiago De Cuba ◽  
Actinomycete Strain

During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC15:0, 10 methyl C17:0, anteiso-C17:0 and iso-C17:0. The predominant menaquinones were MK-10(H4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142T (99.5 %), Micromonospora citrea DSM 43903T (99.4 %), Micromonospora marina DSM 45555T (99.4 %), Micromonospora maritima DSM 45782T (99.3 %), Micromonospora sediminicola DSM 45794T (99.3 %), Micromonospora aurantiaca DSM 43813T (99.2 %) and Micromonospora chaiyaphumensis DSM 45246T (99.2 %). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026T (96.4 %). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38T (=LMG 30467T=CECT 30034T) as the type strain of the novel species Micromonospora fluminis sp. nov.

Planobispora siamensis sp. nov., isolated from soil

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2649-2654 ◽  
Author(s):  
Wipaporn Ngaemthao ◽  
Chanwit Suriyachadkun ◽  
Suwanee Chunhametha ◽  
Tomohiko Tamura ◽  
Jean-Jacques Sanglier
Keyword(s):  
Novel Species ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Evergreen Forest ◽  
Rrna Gene ◽  
Content Type ◽  
Mycolic Acids ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Actinomycete Strain

A novel actinomycete strain, A-T 4600T, which developed cylindrical sporangia containing a longitudinal pair of motile spores forming singly or in bundles on short ramifications of the aerial mycelium, was isolated from soil collected from an evergreen forest in Thailand. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinones were MK-9(H2). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannoside, diphosphatidylglycerol and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1, C18 : 1, saturated C16 : 0, and C17 : 0. The G+C content of the DNA was 70.8 mol%. Phenotypic and chemotaxonomic analyses showed that the isolate had the typical characteristics of members of the genus Planobispora . Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belonged to the genus Planobispora but as a putative novel species. DNA–DNA relatedness values that differentiate the isolate from previously described members of the genus Planobispora were significantly below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it is proposed that the isolate represents a novel species, Planobispora siamensis sp. nov.; the type strain is A-T 4600T ( = BCC 39469T = NBRC 107568T).

scholarly journals Streptomyces cheonanensis sp. nov., a novel streptomycete with antifungal activity

2006 ◽  
Vol 56 (2) ◽  
pp. 471-475 ◽  
Author(s):  
Hyo Jin Kim ◽  
Sung Chul Lee ◽  
Byung Kook Hwang
Keyword(s):  
Antifungal Activity ◽  
Gene Sequence ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Physiological And Biochemical Characteristics ◽  
Actinomycete Strain ◽  
Reference Strains ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

Actinomycete strain VC-A46T, which exhibits antifungal activity, was isolated from a soil sample collected from Cheonan, Korea. The strain was determined to belong to the genus Streptomyces, based on its morphological and chemotaxonomic characteristics. The cell wall of this strain contained ll-diaminopimelic acid. The predominant fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 cis 9. The DNA G+C content of strain VC-A46T was 75·5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of this strain revealed that it differed from those of the described streptomycetes available in public databases. Analyses of DNA–DNA relatedness data, together with data regarding the strain's morphological, physiological and biochemical characteristics, also confirmed that this strain constitutes a novel Streptomyces taxon, which is distinguishable from closely related reference strains. Therefore, it is proposed that strain VC-A46T (=KCCM 42119T=NBRC 100940T) represents the type strain of a novel species, named Streptomyces cheonanensis sp. nov.

scholarly journals Streptomyces panacagri sp. nov., isolated from soil of a ginseng field

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 780-785 ◽  
Author(s):  
Yingshun Cui ◽  
Sang-Hoon Baek ◽  
Liang Wang ◽  
Hyung-Gwan Lee ◽  
Changhao Cui ◽  
...  
Keyword(s):  
Cell Wall ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Link Type ◽  
Physiological And Biochemical Characteristics ◽  
Actinomycete Strain ◽  
Physiological And Biochemical

A Gram-positive, spore-forming, aerobic actinomycete, strain Gsoil 519T, was isolated from soil of a ginseng field of Pocheon province in South Korea. The closest phylogenetic relatives were Streptomyces marinus Sp080513GE-26T (97.94 % 16S rRNA gene sequence similarity), Streptomyces albiaxialis NRRL B-24327T (97.84 %), Streptomyces albus subsp. albus DSM 40313T (97.84 %), Streptomyces almquistii NBRC 13015T (97.81 %), Streptomyces gibsonii NBRC 15415T (97.81 %), Streptomyces rangoonensis NBRC 13078T (97.81 %), Streptomyces sodiiphilus YIM 80305T (97.77 %) and Streptomyces flocculus NBRC 13041T (97.67 %). The G+C content of the genomic DNA was 71.8 mol%. The chemotaxonomic data [MK-9(H6) and MK-9(H8) as the major menaquinones; ll-diaminopimelic acid as a component of the cell-wall peptidoglycan; ribose, xylose, mannose and glucose as the major cell-wall sugars; and anteiso-C15 : 0, iso-C15 : 0, iso-C17 : 0, anteiso-C17 : 0 and C16 : 0 as the major fatty acids] supported the affiliation of strain Gsoil 519T to the genus Streptomyces . The physiological and biochemical characteristics and the low level of DNA–DNA relatedness differentiated the isolate genotypically and phenotypically from recognized members of the genus Streptomyces . The isolate, therefore, represents a novel species, for which the name Streptomyces panacagri sp. nov. is proposed, with Gsoil 519T ( = KCTC 19139T = DSM 41871T) as the type strain.

scholarly journals Haloactinopolyspora alkaliphila sp. nov., and emended description of the genus Haloactinopolyspora

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1945-1951 ◽  
Author(s):  
Yong-Guang Zhang ◽  
Qing Liu ◽  
Hong-Fei Wang ◽  
Dao-Feng Zhang ◽  
Yuan-Ming Zhang ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
North West ◽  
Link Type ◽  
Hybridization Data ◽  
Actinomycete Strain ◽  
Diamino Acid

A facultatively alkaliphilic actinomycete strain, designated EGI 80088T, was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088T formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained ll-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5 %) were anteiso-C15 : 0, iso-C15 : 0, summed feature 4 (iso-C17 : 1I/anteiso-C17 : 1B), iso-C16 : 0 and anteiso-C17 : 0. The predominant menaquinone was MK-9(H4). The G+C content of the genomic DNA of strain EGI 80088T was 70.6 mol%. EGI 80088T showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour Haloactinopolyspora alba YIM 93246T (98.5 %). The DNA–DNA relatedness value of the strain EGI 80088T and H. alba YIM 93246T was 59.3±5.2 %. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data, strain EGI 80088T represents a novel species of the genus Haloactinopolyspora , for which the name Haloactinopolyspora alkaliphila sp. nov. (type strain EGI 80088T = BCRC 16946T = JCM 19128T) is proposed. The description of the genus Haloactinopolyspora has also been emended.

scholarly journals Nocardia sungurluensis sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1629-1634 ◽  
Author(s):  
Mustafa Camas ◽  
Aysel Veyisoglu ◽  
Nevzat Sahin
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Gene Tree ◽  
Diaminopimelic Acid ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type

A novel Gram-reaction-positive, rod-shaped, non-motile and mycolic acid-containing strain, CR3272T, isolated from soil, was studied using a polyphasic approach. The organism showed a combination of chemotaxonomic and morphological properties typical of the genus Nocardia . The cell wall contained meso-diaminopimelic acid (type IV) and whole-cell sugars were galactose, glucose, arabinose and xylose. The predominant menaquinone was MK-8(H4cyc). The major phospholipids were diphosphatidylglycerol and phosphatidylinositol mannosides. Major fatty acids were C16 : 0, C18 : 1 cis9, C18 : 0 10-methyl (TBSA) and C16 : 1 cis9. The novel strain formed distinct phyletic line in the Nocardia 16S rRNA gene tree and was closely associated with Nocardia goodfellowii A2012T (98.6 % 16S rRNA gene sequence similarity), Nocardia alba YIM 30243T (98.5 %) and Nocardia caishijiensis F829T (97.9 %). However, DNA–DNA relatedness values and phenotypic data demonstrated that strain CR3272T was clearly distinguished from all closely related species of the genus Nocardia . It is concluded that the organism be classified as representing a novel species of the genus Nocardia , for which the name Nocardia sungurluensis is proposed. The type strain is CR3272T ( = DSM 45714T = KCTC 29094T).

Haloferula chungangensis sp. nov., isolated from marine sediment

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 19-24 ◽  
Author(s):  
Hyeonji Kang ◽  
Jitsopin Traiwan ◽  
Veeraya Weerawongwiwat ◽  
Min Young Jung ◽  
Ji Hoon Jeong ◽  
...  
Keyword(s):  
Marine Sediment ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Distinct Lineage

A Gram-stain-negative, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated CAU 1074T, was isolated from marine sediment and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1074T grew optimally at 30 °C and pH 6.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1074T formed a distinct lineage within the genus Haloferula and was most closely related to Haloferula harenae KCTC 22198T (96.0 % similarity). Strain CAU 1074T contained MK-9 as the major isoprenoid quinone, and iso-C14 : 0, C16 : 1ω9c and C16 : 0 as the major fatty acids. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major whole-cell sugars were glucose, xylose, mannose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminoglycolipid and two unidentified phospholipids. The DNA G+C content of the strain was 64.0 mol%. On the basis of phenotypic and chemotaxonomic data, and phylogenetic inference, strain CAU 1074T should be classified as a member of a novel species in the genus Haloferula , for which the name Haloferula chungangensis sp. nov. is proposed; the type strain is CAU 1074T ( = KCTC 23578T = CCUG 61920T). An emended description of the genus Haloferula is also provided.

scholarly journals Actinophytocola sediminis sp. nov., an actinomycete isolated from a marine sediment

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2834-2840 ◽  
Author(s):  
Dao-Feng Zhang ◽  
Zhao Jiang ◽  
Xiao-Mei Zhang ◽  
Ling-Ling Yang ◽  
Xin-Peng Tian ◽  
...  
Keyword(s):  
Marine Sediment ◽  
Novel Species ◽  
Major Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Substrate Mycelium ◽  
Link Type ◽  
Aerial Hyphae ◽  
The Media

A novel actinomycete strain, designated YIM M13705T, was isolated from a marine sediment sample of the South China Sea and its characteristics were determined by a polyphasic approach. The slowly growing, Gram-stain-positive, aerobic strain produced branched substrate mycelium and aerial hyphae, and no diffusible pigment was produced on the media tested. At maturity, spore chains were formed on aerial hyphae and substrate mycelium was not fragmented. Whole-cell hydrolysates of the strain contained meso-diaminopimelic acid and galactose, glucose, ribose and rhamnose. The predominant menaquinones were MK-9(H4) and MK-10(H2). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and ninhydrin-positive phosphoglycolipids. The major fatty acid was iso-C16 : 0. The G+C content of the genomic DNA was 68.2  mol%. On the basis of 16S rRNA gene sequence, the strain was shown to be most closely related to species of the genus Actinophytocola . DNA–DNA hybridization relatedness values (<70 %) of the isolate with its closest neighbour Actinophytocola xinjiangensis QAIII60T supported classification of the isolate as a representative of a novel species. On the basis of phylogenetic analysis, and phenotypic and genotypic data, it is concluded that the new isolate belongs to a novel species of the genus Actinophytocola , for which the name Actinophytocola sediminis sp. nov. (type strain YIM M13705T = DSM 45939T = BCRC 16956T) is proposed.

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