scholarly journals Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78° N)

2006 ◽  
Vol 56 (3) ◽  
pp. 541-547 ◽  
Author(s):  
Ingvild Wartiainen ◽  
Anne Grethe Hestnes ◽  
Ian R. McDonald ◽  
Mette M. Svenning
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Wetland Soil ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Data ◽  
Phenotypic Differentiation ◽  
Type Strains ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, non-motile, non-spore-forming, pink-pigmented bacterium, SV97T, was isolated from a wetland soil near Ny-Ålesund, Svalbard Islands, Norway (78° N). On the basis of 16S rRNA gene sequence similarity, strain SV97T was shown to belong to the Alphaproteobacteria and was highly related to a number of non-characterized Methylocystis strains with GenBank accession nos AJ458507 and AJ458502 (100 %) and AF177299, AJ458510, AJ458467, AJ458471, AJ431384, AJ458475, AJ458484, AJ458501 and AJ458466 (99 %). The most closely related type strains were Methylocystis parvus OBBPT (97·2 %) and Methylocystis echinoides IMET 10491T (97 %). The closest related recognized species within the genus Methylosinus was Methylosinus sporium NCIMB 11126T (96·0 % similarity). Chemotaxonomic and phenotypic data (C18 : 1 ω8 as the major fatty acid, non-motile, no rosette formation) supported the affiliation of strain SV97T to the genus Methylocystis. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain SV97T from the two recognized Methylocystis species. Strain SV97T therefore represents a novel species, for which the name Methylocystis rosea sp. nov. is proposed, with the type strain SV97T (=DSM 17261T=ATCC BAA-1196T).

scholarly journals Brevundimonas vancanneytii sp. nov., isolated from blood of a patient with endocarditis

2010 ◽  
Vol 60 (9) ◽  
pp. 2129-2134 ◽  
Author(s):  
Andréia B. Estrela ◽  
Wolf-Rainer Abraham
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Sulfoquinovosyl Diacylglycerol ◽  
Type Strains ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, non-spore-forming bacterial strain, designated LMG 2337T, was isolated from the blood of a patient with endocarditis and characterized. The strain was affiliated with the alphaproteobacterial genus Brevundimonas, with Brevundimonas diminuta LMG 2089T (98.3 % 16S rRNA gene sequence similarity) and Brevundimonas terrae KSL-145T (97.5 %) as its closest relatives. This affiliation was supported by chemotaxonomic data: the G+C content was 66.3 mol %, the major polar lipids were phosphatidyl diacylglycerol, sulfoquinovosyl diacylglycerol and phosphatidyl glucopyranosyl diacylglycerol and the major fatty acids were summed feature 7 (one or more of C18 : 1 ω7c, C18 : 1 ω9t and C18 : 1 ω12t) and C16 : 0. Strain LMG 2337T displayed an unusually broad substrate spectrum. The results from DNA–DNA hybridization and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain LMG 2337T from all of the type strains of hitherto-described Brevundimonas species. The strain therefore represents a novel species, for which the name Brevundimonas vancanneytii sp. nov. is proposed, with type strain LMG 2337T (=CCUG 1797T =ATCC 14736T).

scholarly journals Streptomyces qinglanensis sp. nov., isolated from mangrove sediment

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 596-600 ◽  
Author(s):  
Huo Hu ◽  
Hai-Peng Lin ◽  
Qingyi Xie ◽  
Lei Li ◽  
Xin-Qiang Xie ◽  
...  
Keyword(s):  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Data ◽  
Phenotypic Differentiation ◽  
Mangrove Soil ◽  
Type Strains ◽  
Physiological And Biochemical

A Streptomyces-like strain, 172205T, was obtained from mangrove soil collected at Qinglan Harbour, Wenchang, Hainan, China. The strain was characterized by white aerial mycelium and long spore chains. Comparison of 16S rRNA gene sequences indicated that the strain represents a novel member of the genus Streptomyces, exhibiting highest levels of similarity (<98.29 %) to the type strains of members of the genus Streptomyces. However, DNA–DNA relatedness and phenotypic data readily distinguished strain 172205T from phylogenetically related type strains. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major fatty acids were iso-C15 : 0 (10.31 %), anteiso-C15 : 0 (35.19 %), iso-C16 : 0 (20.24 %) and anteiso-C17 : 0 (10.05 %). The diagnostic phospholipid was phosphatidylethanolamine. The cell wall contained ll-diaminopimelic acid and meso-diaminopimelic acid and whole-cell hydrolysates contained ribose, galactose and glucose. The results of DNA–DNA hybridization, physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain 172205T from phylogenetically related type strains. Therefore, strain 172205T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces qinglanensis sp. nov. is proposed. The type strain is 172205T ( = CGMCC 4.6825T  = DSM 42035T).

scholarly journals Citricoccus parietis sp. nov., isolated from a mould-colonized wall and emended description of Citricoccus alkalitolerans Li et al. 2005

2010 ◽  
Vol 60 (2) ◽  
pp. 271-274 ◽  
Author(s):  
Jenny Schäfer ◽  
Karin Martin ◽  
Peter Kämpfer
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Phenotypic Differentiation ◽  
Emended Description ◽  
Diamino Acid ◽  
Physiological And Biochemical ◽  
Sequence Similarities

A Gram-positive, coccoid-shaped organism (strain 02-Je-010T), forming yellow-pigmented colonies was isolated from the wall of an indoor environment. On the basis of 16S rRNA gene sequence similarity studies, it was shown that strain 02-Je-010T belongs to the genus Citricoccus with sequence similarities of 98.9 % to Citricoccus alkalitolerans DSM 15665T and 98.6 % to Citricoccus muralis DSM 14442T. Cell-wall sugars were mannose and glucose. The diagnostic diamino acid of the peptidoglycan was lysine. The major menaquinones detected were MK-9(H2) and MK-8(H2). The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and moderate amounts of two unknown phospholipids and two unknown glycolipids. The fatty acid profile comprised major amounts of anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. All these data supported the affiliation of strain 02-Je-010T to the genus Citricoccus. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 02-Je-010T from the two recognized Citricoccus species. For these reasons, strain 02-Je-010T represents a novel species, for which the name Citricoccus parietis sp. nov. is proposed, with the type strain 02-Je-010T (=CCUG 57388T=CCM 7609T).

scholarly journals Halorubrum tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China

2004 ◽  
Vol 54 (4) ◽  
pp. 1213-1216 ◽  
Author(s):  
Huapeng Fan ◽  
Yanfen Xue ◽  
Yanhe Ma ◽  
Antonio Ventosa ◽  
William D. Grant
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Tibetan Plateau ◽  
Phylogenetic Distance ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Gene Sequence Analysis ◽  
Derivatives Of ◽  
Physiological And Biochemical

A novel haloalkaliphilic archaeon, strain 8W8T, was isolated from Lake Zabuye, on the Tibetan Plateau, China. On the basis of 16S rRNA gene sequence analysis, strain 8W8T was shown to belong to the genus Halorubrum and was related to Halorubrum vacuolatum (96·7 % sequence similarity), Halorubrum saccharovorum (96·0 %), Halorubrum lacusprofundi (95·4 %) and Halorubrum sodomense (95·3 %). The phylogenetic distance from any species within the other genera of Halobacteriales was lower than 90 %. The major polar lipids of strain 8W8T were C20C20 and C20C25 derivatives of phosphatidylglycerol phosphate and phosphatidylglycerol phosphate methyl ester. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 8W8T from the eight Halorubrum species with validly published names. Therefore, strain 8W8T represents a novel species, for which the name Halorubrum tibetense sp. nov. is proposed, with the type strain 8W8T (=AS 1.3239T=JCM 11889T).

scholarly journals Devosia limi sp. nov., isolated from a nitrifying inoculum

2005 ◽  
Vol 55 (5) ◽  
pp. 1997-2000 ◽  
Author(s):  
Bram Vanparys ◽  
Kim Heylen ◽  
Liesbeth Lebbe ◽  
Paul De Vos
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Sds Page ◽  
Spore Forming Bacteria ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, non-spore-forming bacteria was isolated from a nitrifying inoculum. On the basis of 16S rRNA gene sequence similarity, this strain, designated LMG 22951T, was shown to belong to the ‘Alphaproteobacteria’ and to be related to Devosia neptuniae (97·4 %) and Devosia riboflavina (97·0 %). The results of DNA–DNA hybridization, analysis of fatty acid composition, SDS-PAGE, physiological and biochemical tests allowed genotypic and phenotypic differentiation of LMG 22951T from the two recognized Devosia species. LMG 22951T therefore represents a novel species within this genus, for which the name Devosia limi is proposed. The type strain is LMG 22951T (=DSM 17137T).

scholarly journals Herminiimonas saxobsidens sp. nov., isolated from a lichen-colonized rock

2007 ◽  
Vol 57 (11) ◽  
pp. 2618-2622 ◽  
Author(s):  
Elke Lang ◽  
Jolantha Swiderski ◽  
Erko Stackebrandt ◽  
P. Schumann ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rock Surface ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Bacterium Strain ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, non-spore-forming bacterium (strain NS11T) was isolated from a lichen-colonized rock surface. On the basis of 16S rRNA gene sequence similarity, strain NS11T was shown to belong to the Betaproteobacteria, and was most closely related to Herminiimonas arsenicoxydans ULPAs1T (98.8 %), Herminiimonas aquatilis CCUG 36956T (98.0 %) and Herminiimonas fonticola S-94T (98.0 %). Major whole-cell fatty acids were C16 : 0, C17 : 0 cyclo and C16 : 1 ω7c. Strain NS11T also contained high proportions of C10 : 0 3-OH and C18 : 1 ω7c. This pattern is typical for members of the genus Herminiimonas. The results of DNA–DNA hybridization experiments and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain NS11T from the three recognized Herminiimonas species. It is therefore concluded that strain NS11T represents a novel species of the genus Herminiimonas, for which the name Herminiimonas saxobsidens sp. nov. is proposed. The type strain is NS11T (=DSM 18748T=CCM 7436T).

scholarly journals Burkholderia terrae sp. nov., isolated from a forest soil

2006 ◽  
Vol 56 (2) ◽  
pp. 453-457 ◽  
Author(s):  
Hee-Chan Yang ◽  
Wan-Taek Im ◽  
Kwang Kyu Kim ◽  
Dong-Shan An ◽  
Sung-Taik Lee
Keyword(s):  
Forest Soil ◽  
Dna Hybridization ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phylogenetic Distance ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
The Family ◽  
Curved Rod ◽  
Physiological And Biochemical

A Gram-negative, slightly curved rod-shaped bacterium, designated strain KMY02T, was isolated from a forest soil in Daejeon, South Korea. On the basis of 16S rRNA gene sequence similarity, strain KMY02T was shown to belong to the family Burkholderiaceae of the Betaproteobacteria, and to be related most closely to Burkholderia hospita LMG 20598T (98·7 %), Burkholderia caribensis LMG 18531T (98·0 %) and Burkholderia phymatum LMG 21445T (97·4 %). Its phylogenetic distance from all recognized species within the genus Burkholderia was less than 97 %. Chemotaxonomic data [Q-8 as the major ubiquinone; C16 : 0, C17 : 0 cyclo, summed feature 7 (C18 : 1 ω7c/ω9t/ω12t) and C15 : 0 as the major fatty acids] supported the affiliation of strain KMY02T to the genus Burkholderia. The results of DNA–DNA hybridization experiments and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the strain from recognized Burkholderia species. Therefore, KMY02T (=KCTC 12388T=NBRC 100964T) represents the type strain of a novel species, for which the name Burkholderia terrae sp. nov. is proposed.

scholarly journals Polaromonas aquatica sp. nov., isolated from tap water

2006 ◽  
Vol 56 (3) ◽  
pp. 605-608 ◽  
Author(s):  
Peter Kämpfer ◽  
Hans-Jürgen Busse ◽  
Enevold Falsen
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Tap Water ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Spore Forming Bacteria ◽  
Physiological And Biochemical

Two Gram-negative, rod-shaped, non-spore-forming bacteria (CCUG 39402T and CCUG 39797), isolated from different water sources, were investigated in a polyphasic study. The two isolates shared 100 % 16S rRNA gene sequence similarity and it was shown that they belonged to the Betaproteobacteria, most closely related to Polaromonas vacuolata (97·8 %) and Polaromonas naphthalenivorans (97·8 %). A polyamine pattern with 2-hydroxyputrescine and putrescine, as well as ubiquinone Q-8, were in agreement with characteristics of Betaproteobacteria. The presence of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and major fatty acids C16 : 1 ω7c, C16 : 0 and C17 : 0 cyclo supported the affiliation of the two strains to the genus Polaromonas. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the two isolates from the two Polaromonas species with validly published names. They therefore represent a novel species, for which the name Polaromonas aquatica sp. nov. is proposed, with the type strain CCUG 39402T (=CIP 108776T).

scholarly journals Actinophytocola burenkhanensis sp. nov., isolated from Mongolian soil

2011 ◽  
Vol 61 (5) ◽  
pp. 1033-1038 ◽  
Author(s):  
Ismet Ara ◽  
Baljinova Tsetseg ◽  
Damdinsuren Daram ◽  
Manabu Suto ◽  
Katsuhiko Ando
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Actinomycete Strain ◽  
Physiological And Biochemical

A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0203T, that formed pale yellow to orange-brown colonies and non-fragmented branched substrate mycelium is described. The strain, which produced very scanty aerial mycelium-like structures and scanty formation of spherical bodies on the aerial mycelium on Bennett’s agar medium, was studied in detail to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity studies, strain MN08-A0203T grouped with the genus Actinophytocola, being most closely related to the type strain of Actinophytocola oryzae (97.8 %). Chemotaxonomic data [menaquinone MK-9(H4); iso-C16 : 0 (27 %), iso-C15 : 0 (18 %), C16 : 1 H (8 %), C16 : 0 9-methyl (8 %) as major fatty acids; glucose, galactose, ribose, arabinose, mannose, rhamnose and xylose (trace) as whole cell sugars; diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids as polar phospholipids] supported allocation of the strain to the genus Actinophytocola. Furthermore, the results of DNA–DNA hybridization of strain MN08-A0203T with the type strain of Actinophytocola oryzae revealed that the two strains were genetically distinct from each other. Moreover, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain MN08-A0203T from closely related species. Thus, MN08-A0203T represents a novel species of the genus Actinophytocola, for which the name Actinophytocola burenkhanensis sp. nov. is proposed, with MN08-A0203T ( = NBRC 105883T  = VTCC D9-23T) as the type strain.

scholarly journals Rhodovulum visakhapatnamense sp. nov.

2007 ◽  
Vol 57 (8) ◽  
pp. 1762-1764 ◽  
Author(s):  
T. N. R. Srinivas ◽  
P. Anil Kumar ◽  
Ch. Sasikala ◽  
Ch. V. Ramana ◽  
J. F. Imhoff
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Rhodovulum Sulfidophilum ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, phototrophic bacterium (JA181T) was isolated from a tidal water sample. On the basis of 16S rRNA gene sequence similarity, strain JA181T was shown to belong to the class Alphaproteobacteria, most closely related to Rhodovulum sulfidophilum (97.8 % similarity to the type strain), Rhodovulum adriaticum (93 %), Rhodovulum robiginosum (93 %), Rhodovulum iodosum (94 %), Rhodovulum imhoffii (94 %), Rhodovulum strictum (95 %), Rhodovulum euryhalinum (94.6 %) and Rhodovulum marinum (94.6 %). DNA–DNA hybridization with Rdv. sulfidophilum DSM 1374T (relatedness of 39 % with strain JA181T) and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain JA181T from the eight Rhodovulum species with validly published names. Strain JA181T therefore represents a novel species, for which the name Rhodovulum visakhapatnamense sp. nov. is proposed (type strain JA181T =JCM 13531T =ATCC BAA-1274T =DSM 17937T).

Sign in / Sign up

Export Citation Format

Share Document