scholarly journals Sphingomonas mucosissima sp. nov. and Sphingomonas desiccabilis sp. nov., from biological soil crusts in the Colorado Plateau, USA

2007 ◽  
Vol 57 (5) ◽  
pp. 1028-1034 ◽  
Author(s):  
G. S. N. Reddy ◽  
Ferran Garcia-Pichel
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Colorado Plateau ◽  
Sequence Similarity ◽  
Biological Soil Crusts ◽  
Phenotypic Characterization ◽  
Rrna Gene ◽  
Dna Relatedness ◽  
Soil Crusts

Two bacterial strains, CP173-2T and CP1DT, were isolated from biological soil crusts (BSCs) collected in the Colorado Plateau, USA. Both strains were pigmented, Gram-negative, non-motile rods and produced abundant mucus. They contained C16 : 0, C18 : 1 ω7c and C14 : 0 2-OH as the predominant cellular fatty acids, ubiquinone-10 as the isoprenoid quinone and sphingoglycolipid. Based on the above characteristics, the isolates were assigned to the family Sphingomonadaceae; 16 rRNA gene signature nucleotides placed them within the genus Sphingomonas. Strains CP173-2T and CP1DT had a 16S rRNA gene sequence similarity of 96.7 % with each other and 91.6–98.9 % sequence similarity with other species in the genus, indicating that they represent two separate, and possibly novel, species. The closest species to strains CP173-2T and CP1DT were, respectively, Sphingomonas dokdonensis (98.9 % gene sequence similarity) and Sphingomonas panni (97.9 %). However, strain CP173-2T exhibited a DNA–DNA relatedness of only 32.5 % with the type strain of S. dokdonensis. Similarly, the DNA–DNA relatedness between strain CP1DT and the type strain of S. panni was only 18 %. Phenotypic characterization supported this low relatedness. On the basis of this evidence, we propose that the new strains represent two novel species, for which the names Sphingomonas mucosissima sp. nov. (with type strain CP173-2T=ATCC BAA-1239T=DSM 17494T) and Sphingomonas desiccabilis sp. nov. (with type strain CP1DT=ATCC BAA-1041T=DSM 16792T) are proposed.

scholarly journals Lactobacillus farraginis sp. nov. and Lactobacillus parafarraginis sp. nov., heterofermentative lactobacilli isolated from a compost of distilled shochu residue

2007 ◽  
Vol 57 (4) ◽  
pp. 708-712 ◽  
Author(s):  
Akihito Endo ◽  
Sanae Okada
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Dna Relatedness ◽  
Clear Separation ◽  
Micro Organisms

Five strains of lactic acid bacteria were isolated from a compost of distilled shochu residue in Japan. The isolates were separated into two groups on the basis of 16S rRNA gene sequence similarity, and two subclusters were formed that comprised micro-organisms closely related to Lactobacillus buchneri, L. diolivorans, L. hilgardii, L. kefiri, L. parabuchneri and L. parakefiri. DNA–DNA relatedness results revealed that the isolates could be separated into two groups, and these groups correlated well with the subclusters generated using the phylogenetic analysis. Moreover, the levels of DNA–DNA relatedness showed clear separation of the two groups from their phylogenetic relatives. Therefore, the two groups represent two novel species, for which the names Lactobacillus farraginis sp. nov. (type strain NRIC 0676T=JCM 14108T=DSM 18382T) and Lactobacillus parafarraginis sp. nov. (type strain NRIC 0677T=JCM 14109T=DSM 18390T) are proposed.

scholarly journals Corynebacterium marinum sp. nov. isolated from coastal sediment

2010 ◽  
Vol 60 (8) ◽  
pp. 1944-1947 ◽  
Author(s):  
Zong-Jun Du ◽  
Elizabeth M. Jordan ◽  
Alejandro P. Rooney ◽  
Guan-Jun Chen ◽  
Brian Austin
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Coastal Sediment ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
Rrna Gene Sequence ◽  
Power Station ◽  
Dna Relatedness

A taxonomic study was performed on strain D7015T, which was isolated from coastal sediment close to a coal-fired power station in Qingdao, China. Cells of strain D7015T were Gram-positive, non-motile, diphtheroid rods that grew in the presence of 0–8 % (w/v) NaCl and at 4–37 °C, with optimum growth at 1 % (w/v) NaCl and 30–32 °C. The DNA G+C content was 65.0 mol%. The major fatty acids were C18 : 1 ω9c (56.18 %), C16 : 0 (38.02 %), C16 : 1 ω7c (4.45 %), C18 : 0 (1.0 %) and C14 : 0 (0.35 %). On the basis of morphological, physiological and phylogenetic characteristics, strain D7015T was classified in the genus Corynebacterium. It exhibited a 16S rRNA gene sequence similarity of 95.9 % and a DNA–DNA relatedness value of 20.4 % with Corynebacterium halotolerans DSM 44683T. Strain D7015T was sufficiently different from recognized species of the genus Corynebacterium to be considered to represent a novel species. The name Corynebacterium marinum sp. nov. is proposed, with strain D7015T (=CGMCC 1.6998T=NRRL B-24779T) as the type strain.

scholarly journals Lactobacillus paracollinoides sp. nov., isolated from brewery environments

2004 ◽  
Vol 54 (1) ◽  
pp. 115-117 ◽  
Author(s):  
Koji Suzuki ◽  
Wataru Funahashi ◽  
Masahiro Koyanagi ◽  
Hiroshi Yamashita
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Novel ◽  
Rrna Gene Sequence ◽  
Dna Relatedness ◽  
Beer Spoilage

Three novel strains isolated from brewery environments are described. These strains were Gram-positive, facultatively anaerobic, heterofermentative rods that did not exhibit catalase activity. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that these strains belong to the genus Lactobacillus and are most closely related to Lactobacillus collinoides (approximately 99 % similarity). The novel strains could be differentiated from L. collinoides on the basis of DNA–DNA relatedness, differences in beer-spoilage ability and the inability to utilize d-fructose. These isolates represent a novel species, for which the name Lactobacillus paracollinoides sp. nov. is proposed. The type strain is LA2T (=DSM 15502T=JCM 11969T).

scholarly journals Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla

2005 ◽  
Vol 55 (4) ◽  
pp. 1715-1719 ◽  
Author(s):  
Ingrid Groth ◽  
Peter Schumann ◽  
Barbara Schütze ◽  
Juan M. Gonzalez ◽  
Leonila Laiz ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Phenotypic Characterization ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
Rrna Gene Sequence ◽  
Dna Relatedness ◽  
The 16S Rrna Gene

In order to clarify the taxonomic position of an actinobacterium from the Roman catacomb of Domitilla, a combination of phenotypic characterization, phylogenetic analysis based on the 16S rRNA gene sequence and DNA–DNA relatedness studies was used. The results from the polyphasic taxonomic study of this organism showed that strain HKI 0342T (=DSM 16849T=NCIMB 14033T) should be considered as the type strain of a novel species of the genus Isoptericola, for which the name Isoptericola hypogeus sp. nov. is proposed.

scholarly journals Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China

2005 ◽  
Vol 55 (1) ◽  
pp. 149-152 ◽  
Author(s):  
Jie Feng ◽  
Peijin Zhou ◽  
Yu-Guang Zhou ◽  
Shuang-Jiang Liu ◽  
Kimberly Warren-Rhodes
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Soda Lake ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Dna Relatedness

A novel haloalkaliphilic archaeon, strain DZ-1T, was isolated from a soda lake in Xinjiang, China. The taxonomy of strain DZ-1T was studied by polyphasic methods. According to 16S rRNA gene sequence similarity, strain DZ-1T was phylogenetically related to Halorubrum tibetense (97·5 %), Halorubrum vacuolatum (95·7 %) and Halorubrum saccharovorum (95·9 %). Strain DZ-1T was able to grow at 20–44 °C and was also physiologically different from the above-mentioned species with respect to assimilation of sugars and utilization of organic acids. The DNA G+C content of strain DZ-1T was 62·1 mol% (T m). The DNA–DNA relatedness of strain DZ-1T to H. tibetense and H. vacuolatum was 22 and 13 %, respectively. It was concluded that strain DZ-1T represents a novel species of the genus Halorubrum, for which the name Halorubrum alkaliphilum (type strain, DZ-1T=AS 1.3528T=JCM 12358T) is proposed.

scholarly journals Halorubrum xinjiangense sp. nov., a novel halophile isolated from saline lakes in China

2004 ◽  
Vol 54 (5) ◽  
pp. 1789-1791 ◽  
Author(s):  
Jie Feng ◽  
Pei-Jin Zhou ◽  
Shuang-Jiang Liu
Keyword(s):  
16S Rrna ◽  
Saline Lakes ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Rrna Gene Sequence ◽  
Dna Relatedness

A novel halophilic archaeon, strain BD-1T, was isolated from Xiao-Er-Kule Lake in Xinjiang, China. The taxonomy of strain BD-1T was studied by polyphasic methods. According to 16S rRNA gene sequence similarity, strain BD-1T was phylogenetically related to Halorubrum trapanicum (98·4 %), Halorubrum sodomense (98·0 %), Halorubrum distributum (97·8 %) and Halorubrum coriense (97·3 %). Strain BD-1T is able to grow at 10 °C and further differs physiologically from the above species in the assimilation of sugars. The G+C content of DNA is 68·0 % (T m). The DNA–DNA relatedness values to Hrr. trapanicum and Hrr. distributum are 47 and 24 %, respectively. It is concluded that strain BD-1T represents a novel species of the genus Halorubrum, for which the name Halorubrum xinjiangense sp. nov. is proposed. The type strain is BD-1T (=AS 1.3527T=JCM 12388T).

scholarly journals Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials

2005 ◽  
Vol 55 (1) ◽  
pp. 83-85 ◽  
Author(s):  
Akihito Endo ◽  
Sanae Okada
Keyword(s):  
Lactic Acid ◽  
Type Strain ◽  
Lactobacillus Casei ◽  
Gene Sequence ◽  
Novel Species ◽  
Enrichment Culture ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Dna Relatedness

Gram-positive, rod-shaped, motile lactic acid bacteria (strains NRIC 0603, NRIC 0604T, NRIC 0605 and NRIC 0606) were isolated from shochu mashes using an enrichment culture approach. These strains clustered in the Lactobacillus casei–Pediococcus group and were closely related to Lactobacillus nagelii and Lactobacillus mali on the basis of 16S rRNA gene sequence similarity. Levels of DNA–DNA relatedness revealed genotypic separation of the four isolates from the above two species. The isolates are therefore considered to represent a novel species, for which the name Lactobacillus satsumensis is proposed. The type strain is NRIC 0604T (=JCM 12392T=DSM 16230T).

scholarly journals Dyadobacter crusticola sp. nov., from biological soil crusts in the Colorado Plateau, USA, and an emended description of the genus Dyadobacter Chelius and Triplett 2000

2005 ◽  
Vol 55 (3) ◽  
pp. 1295-1299 ◽  
Author(s):  
Gundlapally S. N. Reddy ◽  
Ferran Garcia-Pichel
Keyword(s):  
Type Strain ◽  
Colorado Plateau ◽  
Sequence Similarity ◽  
Stationary Growth Phase ◽  
Biological Soil Crusts ◽  
Rrna Gene ◽  
Stationary Growth ◽  
Soil Crusts ◽  
Gene Sequence Analysis ◽  
Emended Description

Bacterial strain CP183-8T was isolated from biological soil crusts collected in the Colorado Plateau, USA. Cells of this strain were aerobic, non-motile, Gram-negative, psychrotolerant and formed beaded chains in the stationary growth phase. They contained C16 : 1 ω5c and C16 : 1 ω7c as major fatty acids. 16S rRNA gene sequence analysis assigned the strain to the genus Dyadobacter. However, it shared a sequence similarity of only 95·88 % with the type strain of Dyadobacter fermentans, NS114T. Because it also exhibited a significant number of phenotypic and chemotaxonomic differences from D. fermentans, it is described as a novel second species in the genus Dyadobacter, with the name Dyadobacter crusticola sp. nov. The type strain is CP183-8T (=DSM 16708T=ATCC BAA-1036T).

scholarly journals Paenibacillus algorifonticola sp. nov., isolated from a cold spring

2011 ◽  
Vol 61 (9) ◽  
pp. 2167-2172 ◽  
Author(s):  
Qi-Yong Tang ◽  
Na Yang ◽  
Jian Wang ◽  
Yu-Qing Xie ◽  
Biao Ren ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
Rrna Gene Sequence ◽  
Cold Spring ◽  
Xinjiang Uyghur Autonomous Region ◽  
The 16S Rrna Gene

A Gram-stain-positive, endospore-forming, rod-shaped bacterium, designated XJ259T, was isolated from a cold spring sample from Xinjiang Uyghur Autonomous Region, China. The isolate grew optimally at 20–30 °C and pH 7.3–7.8. Comparative analysis of the 16S rRNA gene sequence showed that isolate XJ259T belonged phylogenetically to the genus Paenibacillus, and was most closely related to Paenibacillus xinjiangensis B538T (with 96.6 % sequence similarity), Paenibacillus glycanilyticus DS-1T (96.3 %) and Paenibacillus castaneae Ch-32T (96.1 %), sharing less than 96.0 % sequence similarity with all other members of the genus Paenibacillus. Chemotaxonomic analysis revealing menaquinone-7 (MK-7) as the major isoprenoid quinone, diphosphatidylglycerol, phosphatidylethanolamine and two unknown phosphoglycolipids as the major cellular polar lipids, a DNA G+C content of 47.0 mol%, and anteiso-C15 : 0 and C16 : 0 as the major fatty acids supported affiliation of the new isolate to the genus Paenibacillus. Based on these data, isolate XJ259T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus algorifonticola sp. nov. is proposed. The type strain is XJ259T ( = CGMCC 1.10223T  = JCM 16598T).

scholarly journals Corynebacterium tapiri sp. nov. and Corynebacterium nasicanis sp. nov., isolated from a tapir and a dog, respectively

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3885-3893 ◽  
Author(s):  
Sandra Baumgardt ◽  
Igor Loncaric ◽  
Peter Kämpfer ◽  
Hans-Jürgen Busse
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Established Species

Two Gram-stain-positive bacterial isolates, strain 2385/12T and strain 2673/12T were isolated from a tapir and a dog's nose, respectively. The two strains were rod to coccoid-shaped, catalase-positive and oxidase-negative. The highest 16S rRNA gene sequence similarity identified Corynebacterium singulare CCUG 37330T (96.3 % similarity) as the nearest relative of strain 2385/12T and suggested the isolate represented a novel species. Corynebacterium humireducens DSM 45392T (98.7 % 16S rRNA gene sequence similarity) was identified as the nearest relative of strain 2673/12T. Results from DNA–DNA hybridization with the type strain of C. humireducens demonstrated that strain 2673/12T also represented a novel species. Strain 2385/12T showed a quinone system consisting predominantly of menaquinones MK-8(H2) and MK-9(H2) whereas strain 2673/12T contained only MK-8(H2) as predominant quinone. The polar lipid profiles of the two strains showed the major compounds phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. Phosphatidylinositol was identified as another major lipid in 2673/12T whereas it was only found in moderate amounts in strain 2385/12T. Furthermore, moderate to minor amounts of phosphatidylinositol-mannoside, β-gentiobiosyl diacylglycerol and variable counts of several unidentified lipids were detected in the two strains. Both strains contained corynemycolic acids. The polyamine patterns were characterized by the major compound putrescine in strain 2385/12T and spermidine in strain 2673/12T. In the fatty acid profiles, predominantly C18 : 1ω9c and C16 : 0 were detected. The two strains are distinguishable from each other and the nearest related established species of the genus Corynebacterium phylogenetically and phenotypically. In conclusion, two novel species of the genus Corynebacterium are proposed, namely Corynebacterium tapiri sp. nov. (type strain, 2385/12T = CCUG 65456T = LMG 28165T) and Corynebacterium nasicanis sp. nov. (type strain, 2673/12T = CCUG 65455T = LMG 28166T).

Sign in / Sign up

Export Citation Format

Share Document