scholarly journals Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China

2007 ◽  
Vol 57 (3) ◽  
pp. 485-488 ◽  
Author(s):  
Sanqing Yuan ◽  
Peigen Ren ◽  
Jun Liu ◽  
Yanfen Xue ◽  
Yanhe Ma ◽  
...  
Keyword(s):  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Neutral Salt ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

A Gram-positive, rod-shaped, spore-forming and moderately halophilic bacterium (strain 8-1T) was isolated from a sediment sample of a neutral salt lake in Xin-Jiang, China. The strain grew optimally at 30 °C, pH 7.0–7.5 and 8–12 % (w/v) NaCl. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The predominant isoprenoid quinone was MK-7 and the cell-wall peptidoglycan contained meso-diaminopimelic acid. Phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids were found to be the major polar lipid components. The genomic DNA G+C content was 43.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 8-1T was closely related to Lentibacillus salarius KCTC 3911T (98.0 % sequence similarity) and other recognized species within the genus Lentibacillus (94.5–95.9 %). The level of DNA–DNA relatedness between strain 8-1T and L. salarius KCTC 3911T was 40 %. Based on the phenotypic and genotypic data presented, strain 8-1T is considered to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus halodurans sp. nov. is proposed. The type strain is 8-1T (=CGMCC 1.3702T=DSM 18342T).

scholarly journals Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China

2005 ◽  
Vol 55 (5) ◽  
pp. 1805-1809 ◽  
Author(s):  
Jee-Min Lim ◽  
Che Ok Jeon ◽  
Sung-Min Song ◽  
Jae-Chan Lee ◽  
Yoon Jung Ju ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

An aerobic, spore-forming, moderately halophilic bacterium, strain BH260T, was isolated from a salt lake in China. Cells of this strain were found to be motile rods with flagella. The organism grew optimally at 30–32 °C and pH 8·0 in the presence of 12–15 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a clade with the type strains of the genus Lentibacillus. The levels of 16S rRNA gene sequence similarity for strain BH260T with respect to Lentibacillus salsus KCTC 3911T, Lentibacillus juripiscarius JCM 12147T and Lentibacillus salicampi KCCM 41560T were 96·0, 95·5 and 95·3 %, respectively. Chemotaxonomic data (a DNA G+C content of 44 mol%; MK-7 as the major isoprenoid quinone; A1γ-meso-diaminopimelic acid as the cell-wall type; phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids; anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 as the major fatty acids) also supported assignment of the isolate to the genus Lentibacillus. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus lacisalsi sp. nov. is proposed. The type strain is BH260T (=KCTC 3915T=DSM 16462T).

scholarly journals Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China

2006 ◽  
Vol 56 (2) ◽  
pp. 373-377 ◽  
Author(s):  
Jee-Min Lim ◽  
Che Ok Jeon ◽  
Sang-Mi Lee ◽  
Jae-Chan Lee ◽  
Li-Hua Xu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

A moderately halophilic bacterium, strain BH169T, capable of growing at salinities of 3–20 % (w/v) NaCl was isolated from a saline lake in China. Strain BH169T was strictly aerobic, short-rod-shaped and non-motile (non-flagellated). Its major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The genomic DNA G+C content was about 43 mol% and the predominant quinone was MK-7. The cell-wall peptidoglycan was of the A1γ type, containing meso-diaminopimelic acid as the diagnostic diamino acid. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a distinct phylogenetic line within the spore-forming rods of the genus Bacillus. The levels of 16S rRNA gene sequence similarity to the type strains of Bacillus species were below 93 %. On the basis of phenotypic and molecular properties, strain BH169T (=KCTC 3912T=DSM 16461T) represents the type strain of a novel species within the genus Bacillus, for which the name Bacillus salarius sp. nov. is proposed.

scholarly journals Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China

2005 ◽  
Vol 55 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Pei-Gen Ren ◽  
Pei-Jin Zhou
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacteria ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Neutral Salt ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

Two Gram-positive, rod-shaped, spore-forming and moderately halophilic bacteria (strains 28-1T, 28-4), isolated from a soil sample from a neutral salt lake in Xin-Jiang, China, were characterized polyphasically. On the basis of fasta (ungapped) analyses of 16S rRNA gene sequences, strains 28-1T and 28-4 were shown to belong to the Bacillaceae and to be closely related to Filobacillus milensis DSM 13259T (97·0 %) and Bacillus haloalkaliphilus DSM 5271T (95·7 %). 16S rRNA gene sequence similarity with other recognized species was not more than 94·1 %. Phylogenetic, chemotaxonomic, physiological and biochemical data supported the differentiation of these novel strains from F. milensis and B. haloalkaliphilus. Therefore these two previously unidentified strains are considered to represent a new genus and species, for which the name Tenuibacillus multivorans gen. nov., sp. nov. is proposed. The type strain is 28-1T (=AS 1.3442T=NBRC 100370T).

scholarly journals Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus

2005 ◽  
Vol 55 (3) ◽  
pp. 1027-1031 ◽  
Author(s):  
Jee-Min Lim ◽  
Che Ok Jeon ◽  
Dong-Jin Park ◽  
Hye-Ryoung Kim ◽  
Byoung-Jun Yoon ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

A moderately halophilic, Gram-positive, rod-shaped bacterium (BH030004T) was isolated from a solar saltern in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BH030004T belonged to the genus Pontibacillus. Chemotaxonomic data (DNA G+C content, 42 mol%; major isoprenoid quinone, MK-7; cell-wall type, A1γ-type meso-diaminopimelic acid; major fatty acids, iso-C15 : 0 and anteiso-C15 : 0) also supported the affiliation of the isolate to the genus Pontibacillus. Although the 16S rRNA gene sequence similarity between strain BH030004T and Pontibacillus chungwhensis DSM 16287T was relatively high (99·1 %), physiological properties and DNA–DNA hybridization (about 7 % DNA–DNA relatedness) allowed genotypic and phenotypic differentiation of strain BH030004T from the type strain of P. chungwhensis. Therefore, strain BH030004T represents a novel species of the genus Pontibacillus, for which the name Pontibacillus marinus sp. nov. is proposed. The type strain is BH030004T (=KCTC 3917T=DSM 16465T).

Halomonas xianhensis sp. nov., a moderately halophilic bacterium isolated from a saline soil contaminated with crude oil

2012 ◽  
Vol 62 (1) ◽  
pp. 173-178 ◽  
Author(s):  
Baisuo Zhao ◽  
Hui Wang ◽  
Xinwei Mao ◽  
Ruirui Li ◽  
Yun-Jiao Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Crude Oil ◽  
Saline Soil ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

A Gram-negative, aerobic, short rod-shaped and non-motile bacterium, strain A-1T, was isolated from a saline soil contaminated with crude oil in Xianhe, Shangdong Province, China. Strain A-1T formed yellow colonies, was moderately halophilic and grew with 0.05–27.5 % (w/v) total salts (optimum 5–8 %), at 10–42 °C (optimum 30 °C) and at pH 5.5–9.0 (optimum pH 7.2). The dominant fatty acids (>5 %) were C16 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), C18 : 1ω7c, C19 : 0 cyclo ω8c and C12 : 0 3-OH and the predominant ubiquinone was Q-9. The genomic DNA G+C content was 67.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A-1T belonged to the genus Halomonas in the class Gammaproteobacteria. The closest relatives were Halomonas lutea YIM 91125T (97.7 % 16S rRNA gene sequence similarity), H. muralis LMG 20969T (95.6 %), H. pantelleriensis AAPT (95.5 %) and H. kribbensis BH843T (95.2 %). DNA–DNA relatedness between strain A-1T and H. lutea CCTCC AB 206093T was 27±3 %. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain A-1T should be placed in the genus Halomonas as a representative of a novel species. The name Halomonas xianhensis sp. nov. is proposed, with strain A-1T ( = CGMCC 1.6848T  = JCM 14849T) as the type strain.

scholarly journals Halomonas koreensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea

2004 ◽  
Vol 54 (6) ◽  
pp. 2037-2042 ◽  
Author(s):  
Jee-Min Lim ◽  
Jung-Hoon Yoon ◽  
Jae-Chan Lee ◽  
Che Ok Jeon ◽  
Dong-Jin Park ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain ◽  
Type Strains

A moderately halophilic bacterium, strain SS20T, capable of growing at salinities of 1–20 % (w/v) NaCl was isolated from a solar saltern of the Dangjin area in Korea and was characterized taxonomically. Strain SS20T was a Gram-negative bacterium comprising motile, short rods. Its major cellular fatty acids were C18 : 1 ω7c, C19 : 0 ω8c cyclo and C16 : 0. The DNA G+C content was 70 mol% and the predominant ubiquinone was Q-9. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SS20T belonged to the genus Halomonas. The levels of 16S rRNA gene sequence similarity to the type strains of Halomonas species were in the range 93·0–97·5 %. The levels of DNA–DNA relatedness between strain SS20T and the type strains of phylogenetically closely related Halomonas species were in the range 5·3–12·3 %. On the basis of physiological and molecular properties, strain SS20T represents a novel species of the genus Halomonas, for which the name Halomonas koreensis sp. nov. is proposed. The type strain is SS20T (=KCTC 12127T=JCM 12237T).

Lentibacillus jeotgali sp. nov., a halophilic bacterium isolated from traditional Korean fermented seafood

2010 ◽  
Vol 60 (5) ◽  
pp. 1017-1022 ◽  
Author(s):  
Mi-Ja Jung ◽  
Seong Woon Roh ◽  
Min-Soo Kim ◽  
Jin-Woo Bae
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

A novel, Gram-positive, non-motile, endospore-forming and moderately halophilic bacterium, strain GrbiT, was isolated from a traditional Korean fermented seafood. The organism grew optimally in the presence of 10–15 % NaCl, at 37 °C and pH 8.0. The peptidoglycan of the cell wall consisted of meso-diaminopimelic acid, and the predominant menaquinone was MK-7. The major fatty acids of strain GrbiT were iso-C16 : 0 (36.4 %), anteiso-C15 : 0 (30.3 %) and iso-C14 : 0 (18.2 %). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. The genomic DNA G+C content was 42.5 mol%. Strain GrbiT was most closely related to the type strain Lentibacillus kapialis JCM 12580T, with which it shared 97.5 % 16S rRNA gene sequence similarity. The DNA–DNA hybridization value between strains GrbiT and L. kapialis JCM 12580T was 8 %. Based on phenotypic, genotypic and phylogenetic data, strain GrbiT should be classified as a novel species within the genus Lentibacillus, for which the name Lentibacillus jeotgali sp. nov. is proposed. The type strain is GrbiT (=KCTC 13300T=JCM 15795T).

scholarly journals Virgibacillus subterraneus sp. nov., a moderately halophilic Gram-positive bacterium isolated from subsurface saline soil

2010 ◽  
Vol 60 (12) ◽  
pp. 2763-2767 ◽  
Author(s):  
Xiaowei Wang ◽  
Yanfen Xue ◽  
Yanhe Ma
Keyword(s):  
Gene Sequence ◽  
Saline Soil ◽  
Qaidam Basin ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

A Gram reaction-positive, moderately halophilic bacterium, designated H57B72T, was isolated from subsurface saline soil of Qaidam basin in the Qinghai province, China. Cells were rod-shaped, strictly aerobic, spore-forming and motile. The isolate grew optimally at 9 % (w/v) NaCl, pH 7.5 and 30 °C. The cell-wall peptidoglycan of strain H57B72T contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0 (59.97 %) and anteiso-C17 : 0 (17.14 %). Phosphatidylglycerol, diphosphatidylglycerol and a glycolipid were found to be the predominant polar lipids. The genomic DNA G+C content of strain H57B72T was 37.1 mol%. 16S rRNA gene sequence analysis showed that strain H57B72T was a member of the genus Virgibacillus and was most closely related to Virgibacillus salinus DSM 21756T (98.3 % gene sequence similarity). The level of DNA–DNA relatedness between strain H57B72T and V. salinus DSM 21756T was 8.5 %. Based on the phenotypic, genotypic and phylogenetic data presented, strain H57B72T represents a novel species, for which the name Virgibacillus subterraneus sp. nov. is proposed. The type strain is H57B72T (=DSM 22441T =CGMCC 1.7734T).

scholarly journals Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China

2006 ◽  
Vol 56 (3) ◽  
pp. 599-604 ◽  
Author(s):  
I. J. Carrasco ◽  
M. C. Márquez ◽  
X. Yanfen ◽  
Y. Ma ◽  
D. A. Cowan ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Analysis ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

Three Gram-positive, moderately halophilic, spore-forming rods, designated XH-63T, XH-62 and EJ-15, were isolated from two salt lakes located near Xilin Hot and Ejinor, in Inner Mongolia, China. The strains were strictly aerobic and motile, with spherical, terminal and deforming endospores. They grew at pH 5·0–9·0 (optimal growth at pH 7·5), between 4 and 45 °C (optimal growth at 37 °C) and at salinities of 1–20 % (w/v) total salts, growing optimally at 10 % (w/v) salts. They had meso-diaminopimelic acid in the cell wall peptidoglycan and DNA G+C contents of 36·1–37·1 mol%. The polar lipid pattern of strain XH-63T, selected as the representative strain, consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and a phospholipid and two amino phospholipids of unknown structure. This strain possessed anteiso-C15 : 0 and anteiso-C17 : 0 as the major fatty acids (altogether representing 72·5 % of total) and MK-7 as the major menaquinone. 16S rRNA gene analysis of the three strains showed that they were within the Gracilibacillus cluster, with highest sequence similarity (95·4–95·8 %) with Gracilibacillus dipsosauri. Based on a combination of phenotypic, chemotaxonomic and phylogenetic features, it is proposed that the three isolates represent a novel species of the genus Gracilibacillus, Gracilibacillus orientalis sp. nov. The type strain is strain XH-63T (=CCM 7326T=AS 1.4250T=CECT 7097T).

Salsuginibacillus kocurii gen. nov., sp. nov., a moderately halophilic bacterium from soda-lake sediment

2007 ◽  
Vol 57 (10) ◽  
pp. 2381-2386 ◽  
Author(s):  
I. J. Carrasco ◽  
M. C. Márquez ◽  
Y. Xue ◽  
Y. Ma ◽  
D. A. Cowan ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Soda Lake ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Novel Genus And Species ◽  
Study Support ◽  
Inner Mongolia Autonomous Region

A Gram-positive, endospore-forming, alkali-tolerant, moderately halophilic bacterium, designated strain CH9dT, was isolated from the sediment of Lake Chagannor in the Inner Mongolia Autonomous Region, China. The cells were rod-shaped and motile. Isolate CH9dT grew at pH 5.8–10.0 (optimally at pH 8.5), at salinities of 3–20 % (w/v) marine salts (optimally at 10.0 %, w/v) and between 20 and 50 °C (optimally at 37 °C). The cell wall contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids of strain CH9dT were anteiso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0 and iso-C15 : 0 and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unknown phospholipids. The G+C content of the DNA was 44.7 mol%. Strain CH9dT exhibited a 16S rRNA gene sequence similarity value of only 91 % with respect to Thalassobacillus devorans DSM 16966T and showed values below 91 % with respect to members of the genera Bacillus, Halobacillus and Marinococcus. Strain CH9dT could be clearly differentiated from its closest phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, data from the polyphasic study support the placement of strain CH9dT in a novel genus and species, for which the name Salsuginibacillus kocurii gen. nov., sp. nov. is proposed. The type strain is CH9dT (=CCM 7365T=CECT 7154T=CGMCC 1.6287T=DSM 18087T).

Sign in / Sign up

Export Citation Format

Share Document