scholarly journals Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China

2006 ◽  
Vol 56 (3) ◽  
pp. 599-604 ◽  
Author(s):  
I. J. Carrasco ◽  
M. C. Márquez ◽  
X. Yanfen ◽  
Y. Ma ◽  
D. A. Cowan ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Analysis ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

Three Gram-positive, moderately halophilic, spore-forming rods, designated XH-63T, XH-62 and EJ-15, were isolated from two salt lakes located near Xilin Hot and Ejinor, in Inner Mongolia, China. The strains were strictly aerobic and motile, with spherical, terminal and deforming endospores. They grew at pH 5·0–9·0 (optimal growth at pH 7·5), between 4 and 45 °C (optimal growth at 37 °C) and at salinities of 1–20 % (w/v) total salts, growing optimally at 10 % (w/v) salts. They had meso-diaminopimelic acid in the cell wall peptidoglycan and DNA G+C contents of 36·1–37·1 mol%. The polar lipid pattern of strain XH-63T, selected as the representative strain, consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and a phospholipid and two amino phospholipids of unknown structure. This strain possessed anteiso-C15 : 0 and anteiso-C17 : 0 as the major fatty acids (altogether representing 72·5 % of total) and MK-7 as the major menaquinone. 16S rRNA gene analysis of the three strains showed that they were within the Gracilibacillus cluster, with highest sequence similarity (95·4–95·8 %) with Gracilibacillus dipsosauri. Based on a combination of phenotypic, chemotaxonomic and phylogenetic features, it is proposed that the three isolates represent a novel species of the genus Gracilibacillus, Gracilibacillus orientalis sp. nov. The type strain is strain XH-63T (=CCM 7326T=AS 1.4250T=CECT 7097T).

scholarly journals Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China

2006 ◽  
Vol 56 (2) ◽  
pp. 373-377 ◽  
Author(s):  
Jee-Min Lim ◽  
Che Ok Jeon ◽  
Sang-Mi Lee ◽  
Jae-Chan Lee ◽  
Li-Hua Xu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

A moderately halophilic bacterium, strain BH169T, capable of growing at salinities of 3–20 % (w/v) NaCl was isolated from a saline lake in China. Strain BH169T was strictly aerobic, short-rod-shaped and non-motile (non-flagellated). Its major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The genomic DNA G+C content was about 43 mol% and the predominant quinone was MK-7. The cell-wall peptidoglycan was of the A1γ type, containing meso-diaminopimelic acid as the diagnostic diamino acid. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a distinct phylogenetic line within the spore-forming rods of the genus Bacillus. The levels of 16S rRNA gene sequence similarity to the type strains of Bacillus species were below 93 %. On the basis of phenotypic and molecular properties, strain BH169T (=KCTC 3912T=DSM 16461T) represents the type strain of a novel species within the genus Bacillus, for which the name Bacillus salarius sp. nov. is proposed.

scholarly journals Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China

2005 ◽  
Vol 55 (5) ◽  
pp. 1805-1809 ◽  
Author(s):  
Jee-Min Lim ◽  
Che Ok Jeon ◽  
Sung-Min Song ◽  
Jae-Chan Lee ◽  
Yoon Jung Ju ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

An aerobic, spore-forming, moderately halophilic bacterium, strain BH260T, was isolated from a salt lake in China. Cells of this strain were found to be motile rods with flagella. The organism grew optimally at 30–32 °C and pH 8·0 in the presence of 12–15 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a clade with the type strains of the genus Lentibacillus. The levels of 16S rRNA gene sequence similarity for strain BH260T with respect to Lentibacillus salsus KCTC 3911T, Lentibacillus juripiscarius JCM 12147T and Lentibacillus salicampi KCCM 41560T were 96·0, 95·5 and 95·3 %, respectively. Chemotaxonomic data (a DNA G+C content of 44 mol%; MK-7 as the major isoprenoid quinone; A1γ-meso-diaminopimelic acid as the cell-wall type; phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids; anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 as the major fatty acids) also supported assignment of the isolate to the genus Lentibacillus. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus lacisalsi sp. nov. is proposed. The type strain is BH260T (=KCTC 3915T=DSM 16462T).

scholarly journals Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1908-1914 ◽  
Author(s):  
Kaibiao Wang ◽  
Lei Zhang ◽  
Yang Yang ◽  
Yuanyuan Pan ◽  
Lin Meng ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Alkaline Soil ◽  
Respiratory Quinone ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40T, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3–15 % (w/v) (optimum, 8 %, w/v), at pH 7.0–9.0 (optimum, pH 8.0) and at 4–60 °C (optimum, 30 °C). It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40T and the type strains of related species of the genus Halobacillus ranged from 98.8 % (Halobacillus alkaliphilus FP5T) to 97.1 % (Halobacillus kuroshimensis IS-Hb7T). DNA–DNA hybridization relatedness values between strain NEAU-ST10-40T and H. alkaliphilus DSM 18525T, Halobacillus campisalis KCTC 13144T, Halobacillus yeomjeoni DSM 17110T, Halobacillus halophilus DSM 2266T, Halobacillus litoralis DSM 10405T, Halobacillus dabanensis DSM 18199T, Halobacillus salinus DSM 18897T, Halobacillus naozhouensis DSM 21183T, Halobacillus trueperi DSM 10404T and Halobacillus salsuginis DSM 21185T were from 43 ± 1 to 19 ± 1 % (mean ± sd). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40T is considered to represent a novel species, for which the name Halobacillus andaensis sp. nov. is proposed. The type strain is NEAU-ST10-40T ( = CGMCC 1.12153T = DSM 25866T).

scholarly journals Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China

2007 ◽  
Vol 57 (3) ◽  
pp. 485-488 ◽  
Author(s):  
Sanqing Yuan ◽  
Peigen Ren ◽  
Jun Liu ◽  
Yanfen Xue ◽  
Yanhe Ma ◽  
...  
Keyword(s):  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Neutral Salt ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Bacterium Strain

A Gram-positive, rod-shaped, spore-forming and moderately halophilic bacterium (strain 8-1T) was isolated from a sediment sample of a neutral salt lake in Xin-Jiang, China. The strain grew optimally at 30 °C, pH 7.0–7.5 and 8–12 % (w/v) NaCl. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The predominant isoprenoid quinone was MK-7 and the cell-wall peptidoglycan contained meso-diaminopimelic acid. Phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids were found to be the major polar lipid components. The genomic DNA G+C content was 43.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 8-1T was closely related to Lentibacillus salarius KCTC 3911T (98.0 % sequence similarity) and other recognized species within the genus Lentibacillus (94.5–95.9 %). The level of DNA–DNA relatedness between strain 8-1T and L. salarius KCTC 3911T was 40 %. Based on the phenotypic and genotypic data presented, strain 8-1T is considered to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus halodurans sp. nov. is proposed. The type strain is 8-1T (=CGMCC 1.3702T=DSM 18342T).

scholarly journals Virgibacillus subterraneus sp. nov., a moderately halophilic Gram-positive bacterium isolated from subsurface saline soil

2010 ◽  
Vol 60 (12) ◽  
pp. 2763-2767 ◽  
Author(s):  
Xiaowei Wang ◽  
Yanfen Xue ◽  
Yanhe Ma
Keyword(s):  
Gene Sequence ◽  
Saline Soil ◽  
Qaidam Basin ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

A Gram reaction-positive, moderately halophilic bacterium, designated H57B72T, was isolated from subsurface saline soil of Qaidam basin in the Qinghai province, China. Cells were rod-shaped, strictly aerobic, spore-forming and motile. The isolate grew optimally at 9 % (w/v) NaCl, pH 7.5 and 30 °C. The cell-wall peptidoglycan of strain H57B72T contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0 (59.97 %) and anteiso-C17 : 0 (17.14 %). Phosphatidylglycerol, diphosphatidylglycerol and a glycolipid were found to be the predominant polar lipids. The genomic DNA G+C content of strain H57B72T was 37.1 mol%. 16S rRNA gene sequence analysis showed that strain H57B72T was a member of the genus Virgibacillus and was most closely related to Virgibacillus salinus DSM 21756T (98.3 % gene sequence similarity). The level of DNA–DNA relatedness between strain H57B72T and V. salinus DSM 21756T was 8.5 %. Based on the phenotypic, genotypic and phylogenetic data presented, strain H57B72T represents a novel species, for which the name Virgibacillus subterraneus sp. nov. is proposed. The type strain is H57B72T (=DSM 22441T =CGMCC 1.7734T).

scholarly journals Halobacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4434-4440 ◽  
Author(s):  
Su-Jin Kim ◽  
Jae-Chan Lee ◽  
Song-Ih Han ◽  
Kyung-Sook Whang
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Solar Saltern ◽  
Moderately Halophilic ◽  
Gram Staining ◽  
Moderately Halophilic Bacterium ◽  
Type Strains

A Gram-staining-positive, moderately halophilic bacterium, designated strain NGS-2T, was isolated from sediment of a solar saltern pond located in Shinan, Korea. Strain NGS-2T was a strictly aerobic, non-motile rod that grew at pH 5.0–10.0 (optimum, pH 8.0), at 10–30 °C (optimum, 28 °C) and in the presence of 1–20 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain NGS-2T belonged to the genus Halobacillus, with sequence similarity of 98.4–95.8 % to existing type strains, showing the highest sequence similarity to Halobacillus dabanensis D-8T (98.4 %), H. litoralis SL-4T (98.4 %), H. trueperi SL-5T (98.2 %), H. faecis IGA7-4T (98.2 %), H. profundi IS-Hb4T (98.1 %) and H. mangrovi MS10T (98.0 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-N-methylethanolamine and an unknown glycolipid. The cell-wall peptidoglycan was based on l-Orn–d-Asp, the predominant isoprenoid quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C15: 0 and anteiso-C17: 0. The DNA G+C content of the novel isolate was 45.0 mol%. Levels of DNA–DNA relatedness between strain NGS-2T and the type strains of 12 other species of the genus ranged from 32 to 3 %. On the basis of the polyphasic analysis conducted in this study, strain NGS-2T represents a novel species of the genus Halobacillus, for which the name Halobacillus sediminis sp. nov. is proposed. The type strain is NGS-2T ( = KACC 18263T = NBRC 110639T).

scholarly journals Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus

2005 ◽  
Vol 55 (3) ◽  
pp. 1027-1031 ◽  
Author(s):  
Jee-Min Lim ◽  
Che Ok Jeon ◽  
Dong-Jin Park ◽  
Hye-Ryoung Kim ◽  
Byoung-Jun Yoon ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

A moderately halophilic, Gram-positive, rod-shaped bacterium (BH030004T) was isolated from a solar saltern in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BH030004T belonged to the genus Pontibacillus. Chemotaxonomic data (DNA G+C content, 42 mol%; major isoprenoid quinone, MK-7; cell-wall type, A1γ-type meso-diaminopimelic acid; major fatty acids, iso-C15 : 0 and anteiso-C15 : 0) also supported the affiliation of the isolate to the genus Pontibacillus. Although the 16S rRNA gene sequence similarity between strain BH030004T and Pontibacillus chungwhensis DSM 16287T was relatively high (99·1 %), physiological properties and DNA–DNA hybridization (about 7 % DNA–DNA relatedness) allowed genotypic and phenotypic differentiation of strain BH030004T from the type strain of P. chungwhensis. Therefore, strain BH030004T represents a novel species of the genus Pontibacillus, for which the name Pontibacillus marinus sp. nov. is proposed. The type strain is BH030004T (=KCTC 3917T=DSM 16465T).

Aquisalimonas asiatica gen. nov., sp. nov., a moderately halophilic bacterium isolated from an alkaline, saline lake in Inner Mongolia, China

2007 ◽  
Vol 57 (5) ◽  
pp. 1137-1142 ◽  
Author(s):  
M. C. Márquez ◽  
I. J. Carrasco ◽  
Y. Xue ◽  
Y. Ma ◽  
D. A. Cowan ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Type Strain ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Strictly Aerobic ◽  
Moderately Halophilic ◽  
Hybridization Data ◽  
Moderately Halophilic Bacterium

Two novel moderately halophilic, Gram-negative rods (strains CG12T and CG13) were isolated from Lake Chagannor in Inner Mongolia Autonomous Region, China. They were strictly aerobic and motile. They grew at pH 6.0–10.8 (optimally at pH 7.5–8.5), at 20–50 °C (optimally at 37 °C) and at salinities of 1–20 % (w/v) total salts (optimally at 7–10 %, w/v). Phylogenetic analysis of the two strains, based on a comparison of their 16S rRNA genes, led to their classification within the class Gammaproteobacteria, the closest recognized type strain being Alkalispirillum mobile DSM 12769T, with which they were found to share 94.4–94.6 % sequence similarity. On the basis of DNA–DNA hybridization data (showing 100 and 99 % relatedness for each other), the two isolates were found to be members of the same species. The DNA G+C contents of strains CG12T and CG13 were found to be 63.6 and 64.0 mol%, respectively. The major cellular fatty acids of strain CG12T, selected as the representative strain, were C18 : 1 ω7c, C16 : 0 and C12 : 0, and its polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, a phosphoglycolipid and six unidentified phospholipids. On the basis of the polyphasic evidence from this study, strains CG12T and CG13 represent a novel genus and species, for which the name Aquisalimonas asiatica gen. nov., sp. nov. is proposed. The type strain of Aquisalimonas asiatica is CG12T (=CCM 7368T=CECT 7151T=CGMCC 1.6291T=DSM 18102T).

scholarly journals Aquisalimonas halophila sp. nov., a moderately halophilic bacterium isolated from a hypersaline mine

2014 ◽  
Vol 64 (Pt_7) ◽  
pp. 2210-2216 ◽  
Author(s):  
Yun-Jiao Zhang ◽  
Man Jia ◽  
Yong-Chun Ma ◽  
Kai-Yang Lu ◽  
Fei Tian ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A Gram-negative, moderately halophilic, strictly aerobic strain, designated YIM 95345T, was isolated from a soil sample of a hypersaline mine in Yunnan province, PR China, and subjected to a polyphasic taxonomic study. Strain YIM 95345T grew at 15–45 °C (optimum 30–35 °C), 3.0–23.0 % (w/v) NaCl (optimum 10.0–11.0 %, w/v) and pH 6.0–9.0 (optimum pH 7.0–8.0). Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism belongs to the genus Aquisalimonas and exhibited sequence similarity of 96.6 % to the sole type strain Aquisalimonas asiatica CG12T. The predominant isoprenoid quinone was Q-8 and the major fatty acids were C16 : 0, C19 : 0 cyclo ω8c and C18 : 1ω7c. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three aminolipids and three unidentified phospholipids. The G+C content of the genomic DNA was 59.4 mol%. Based on the results of our comparative phylogenetic, chemotaxonomic and physiological analyses, the new isolate is assigned to a novel species of the genus Aquisalimonas , for which the name Aquisalimonas halophila sp. nov. is proposed, with the type strain YIM 95345T ( = DSM 25902T = CCTCC AB 2012043T).

scholarly journals Bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field

2011 ◽  
Vol 61 (6) ◽  
pp. 1344-1349 ◽  
Author(s):  
Rong Shi ◽  
Min Yin ◽  
Shu-Kun Tang ◽  
Jae-Chan Lee ◽  
Dong-Jin Park ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Phenotypic Characters ◽  
Diamino Acid

A novel Gram-stain-positive, motile, strictly aerobic bacterium, designated YIM 93174T, was isolated from a salt field in Korea. Cells of this strain were rod-shaped and formed pale tangerine colonies and grew at pH 6.0–8.0 (optimal growth at pH 7.0), at 15–45 °C (optimum 28–37 °C) and at salinities of 0–10 % (w/v) NaCl (optimum 0–2 % NaCl). Some phenotypic characters allowed differentiation of strain YIM 93174T from its nearest phylogenetic relatives. Comparative 16S rRNA gene sequence analysis showed that strain YIM 93174T belongs to the genus Bacillus, exhibiting the highest level of sequence similarity with the type strain of Bacillus humi (95.7 %), followed by those of Bacillus alkalitelluris (94.9 %) and Bacillus litoralis (94.5 %). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. The cell-wall peptidoglycan was of the A1γ type, containing meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 36.9 mol% and the predominant respiratory quinone was MK-7. The major polar lipids of strain YIM 93174T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unknown phospholipids. On the basis of the evidence from this polyphasic study, strain YIM 93174T represents a novel species of the genus Bacillus, for which the name Bacillus luteolus sp. nov. is proposed, with YIM 93174T ( = DSM 22388T  = KCTC 13210T  = CCTCC AA 208068T) as the type strain.

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