scholarly journals Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted

2020 ◽  
Author(s):  
Faustine Ryckebusch ◽  
Michel Peterschmitt ◽  
Martine Granier ◽  
Nicolas Sauvion
Keyword(s):  
Transmission Rate ◽  
Infected Plant ◽  
Aphid Transmission ◽  
Transmission Efficiency ◽  
Aphis Craccivora ◽  
Viral Dna ◽  
Aphid Vector ◽  
Alfalfa Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

Alfalfa leaf curl virus (ALCV) is the first geminivirus for which aphid transmission was reported. Transmission by Aphis craccivora was determined previously to be highly specific and circulative. Using various complementary techniques, the transmission journey of ALCV was monitored from its uptake from infected plant tissues up to the head of its vector. ALCV was shown to be restricted to phloem tissues using fluorescence in situ hybridization (FISH) and electropenetrography (EPG) monitoring of virus acquisition. Furthermore, the virus is heterogeneously distributed in phloem tissues, as revealed by FISH and quantitative PCR of viral DNA acquired by EPG-monitored aphids. Despite the efficient ingestion of viral DNA, about 106 viral DNA copies per insect in a 15 h feeding period on ALCV-infected plants, the individual maximum transmission rate was 12 %. Transmission success was related to a critical viral accumulation, around 1.6×107 viral DNA copies per insect, a threshold that generally needed more than 48 h to be reached. Moreover, whereas the amount of acquired virus did not decrease over time in the whole aphid body, it declined in the haemolymph and heads. ALCV was not detected in progenies of viruliferous aphids and did not affect aphid fitness. Compared to geminiviruses transmitted by whiteflies or leafhoppers, or to luteoviruses transmitted by aphids, the transmission efficiency of ALCV by A. craccivora is low. This result is discussed in relation to the aphid vector of this geminivirus and the agroecological features of alfalfa, a hardy perennial host plant.

scholarly journals Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted

2020 ◽  
Author(s):  
Faustine Ryckebusch ◽  
Michel Peterschmitt ◽  
Martine Granier ◽  
Nicolas Sauvion
Keyword(s):  
Transmission Rate ◽  
Infected Plant ◽  
Aphid Transmission ◽  
Transmission Efficiency ◽  
Aphis Craccivora ◽  
Viral Dna ◽  
Aphid Vector ◽  
Alfalfa Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

ABSTRACTAlfalfa leaf curl virus (ALCV) is the first geminivirus for which an aphid transmission was reported. Transmission by Aphis craccivora was determined previously to be highly specific and circulative. Using various complementary techniques, the transmission journey of ALCV was monitored from its uptake in an infected plant tissue up to the head of its vector. ALCV was shown to be restricted in the phloem using fluorescent in situ hybridization (FISH) and electropenetrography (EPG) monitoring of virus acquisition. Furthermore, the virus is heterogeneously distributed in the phloem as revealed by FISH and qPCR quantification of the viral DNA acquired by aphids monitored by EPG. In spite of the efficient ingestion of viral DNA, about 106 in a 15-hour feeding on ALCV infected plants, the individual transmission rate was at a maximum of 12%. Transmission success was related to a critical viral accumulation, around 1.6×107 viral DNA copies per insect, a threshold that needs generally more than 48 hours to be reached. Moreover, whereas the amount of acquired virus does not decrease over time in the whole aphid body, it decreased in hemolymph and heads. ALCV was not detected in progenies of viruliferous aphids and had no effect on aphid fitness. Compared to geminiviruses transmitted by whiteflies or leafhoppers or to luteovirus transmitted by aphids, the transmission efficiency of ALCV by A. craccivora is low. This result is discussed in relation to the aphid vector of this geminivirus and the agroecological features of alfalfa, a hardy perennial host plant.

scholarly journals Alfalfa Leaf Curl Virus: an Aphid-Transmitted Geminivirus

2015 ◽  
Vol 89 (18) ◽  
pp. 9683-9688 ◽  
Author(s):  
Philippe Roumagnac ◽  
Martine Granier ◽  
Pauline Bernardo ◽  
Maëlle Deshoux ◽  
Romain Ferdinand ◽  
...  
Keyword(s):  
Genome Organization ◽  
Sequence Similarity ◽  
Aphis Craccivora ◽  
Insect Vector ◽  
Content Type ◽  
First Report ◽  
The Family ◽  
Alfalfa Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

The familyGeminiviridaecomprises seven genera differentiated by genome organization, sequence similarity, and insect vector. Capulavirus, an eighth genus, has been proposed to accommodate two newly discovered highly divergent geminiviruses that presently have no known vector. Alfalfa leaf curl virus, identified here as a third capulavirus, is shown to be transmitted byAphis craccivora.This is the first report of an aphid-transmitted geminivirus.

scholarly journals Alfalfa leaf curl virus is transmitted by Aphis craccivora in a highly specific circulative manner

2019 ◽  
Author(s):  
F. Ryckebusch ◽  
N. Sauvion ◽  
M. Granier ◽  
P. Roumagnac ◽  
M. Peterschmitt
Keyword(s):  
Salivary Gland ◽  
Acyrthosiphon Pisum ◽  
Intracellular Localization ◽  
Aphis Craccivora ◽  
Latent Virus ◽  
Vector Species ◽  
Gut Barrier ◽  
Alfalfa Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

AbstractTwo members of the genus Capulavirus (Geminiviridae) are transmitted by aphids including Alfalfa leaf curl virus (ALCV) transmitted by Aphis craccivora. The capulavirus Euphorbia caput-medusae latent virus was shown here to be transmitted also by A. craccivora, using the population EuphorbiaSA. ALCV was transmissible by several A. craccivora populations including Robinia, but not EuphorbiaSA, reflecting a high transmission specificity. ALCV accumulation and localization were analyzed in whole insects, midguts, hemolymphs, and heads of aphids from both populations and from the non-vector species Acyrthosiphon pisum. A 6-day persistence was observed in A. craccivora populations but not in A. pisum. Vector and non-vector A. craccivora populations could be distinguished by contrasted virus accumulations and midgut intracellular localization. Results confirm that ALCV is transmitted according to a circulative-persistent mode, and are consistent with a gut barrier to the transmission of ALCV in A. pisum and a primary salivary gland barrier in A. craccivora.

scholarly journals Alfalfa leaf curl virus is transmitted by Aphis craccivora in a highly specific circulative manner

Virology ◽  
2020 ◽  
Vol 546 ◽  
pp. 98-108 ◽  
Author(s):  
F. Ryckebusch ◽  
N. Sauvion ◽  
M. Granier ◽  
P. Roumagnac ◽  
M. Peterschmitt
Keyword(s):  
Aphis Craccivora ◽  
Alfalfa Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

Genome characterization of an Argentinean isolate of alfalfa leaf curl virus

2017 ◽  
Vol 163 (3) ◽  
pp. 799-803 ◽  
Author(s):  
Nicolás Bejerman ◽  
Verónica Trucco ◽  
Soledad de Breuil ◽  
Patricia Rodriguez Pardina ◽  
Sergio Lenardon ◽  
...  
Keyword(s):  
Genome Characterization ◽  
Alfalfa Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa

Virology ◽  
2016 ◽  
Vol 493 ◽  
pp. 142-153 ◽  
Author(s):  
Pauline Bernardo ◽  
Brejnev Muhire ◽  
Sarah François ◽  
Maëlle Deshoux ◽  
Penelope Hartnady ◽  
...  
Keyword(s):  
South Africa ◽  
Molecular Characterization ◽  
Latent Virus ◽  
Alfalfa Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Effect of okra plant resistance on transmission rate of okra enation leaf curl virus by its vector whitefly, Bemisia tabaci

2021 ◽  
Vol 13 (SI) ◽  
pp. 63-68
Author(s):  
E. Pasupathi ◽  
M. Murugan ◽  
C. Chinniah ◽  
J. Ramalingam ◽  
G. Karthikeyan ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Plant Resistance ◽  
Transmission Rate ◽  
Resistance Mechanisms ◽  
Plant Viruses ◽  
Symptom Expression ◽  
Virus Symptom ◽  
Leaf Curl Virus ◽  
Effect Of Age ◽  
Leaf Curl

The present study aimed to investigate the effect of age of the okra plants that showed varying whitefly resistance responses on the transmission rate of okra enation leaf curl virus (OELCV) by its vector whitefly Bemisia tabaci. The OELCV infected whitefly adults were collected from whitefly colonies and were challenged on the test okra accessions (Upl mona 2, Co 1, Arka anamika and AE 64) of differential ages which were individually caged (7, 10 and 15 d after germination) with glass chimney and the number of such whiteflies used were at the rate of 2, 4, 6, 8, 10, 12, 14 and 20 adults per plant. Observations were made on the virus symptom expression 30 d after challenge. The efficiency of transmission was determined. The efficiency of transmission of OELCV was the highest (maximum T and P*, 0.80, 1.00 and 0.08, 0.10) when 7 d old seedlings were inoculated (Arka anamika and AE 64 respectively) and transmission had decreased as the age of seedlings increased. The estimated transmission rate for single whitefly (P*) increased with an increase in the number of whiteflies used per plant. Okra plant resistance to B. tabaci significantly changed the transmission rates of OELCV on okra. Understanding the resistance mechanisms of the okra accessions and interactions between plant viruses and their insect host can pave the way for novel approaches to protect plants from virus infection.

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