scholarly journals Epidermophyton floccosum: nucleotide sequence analysis and antifungal susceptibility testing of 40 clinical isolates

2019 ◽  
Vol 68 (11) ◽  
pp. 1655-1663 ◽  
Author(s):  
Saham Ansari ◽  
Bahram Ahmadi ◽  
Maryam Norouzi ◽  
Zohreh Ansari ◽  
Mohammad Hosein Afsarian ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Clinical Isolates ◽  
Nucleotide Sequence Analysis ◽  
Epidermophyton Floccosum

scholarly journals Molecular identification and antifungal susceptibility testing of Pucciniomycotina red yeast clinical isolates from Rio de Janeiro, Brazil

2019 ◽  
Vol 51 (1) ◽  
pp. 95-98
Author(s):  
Fabio Brito-Santos ◽  
Maria Helena Galdino Figueiredo-Carvalho ◽  
Rowena Alves Coelho ◽  
Jean Carlos Almeida de Oliveira ◽  
Raissa Vieira Monteiro ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Rio De Janeiro ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Clinical Isolates ◽  
Red Yeast

scholarly journals Evaluation of Etest and Disk Diffusion Methods Compared with Broth Microdilution Antifungal Susceptibility Testing of Clinical Isolates of Candida spp. against Posaconazole

2007 ◽  
Vol 45 (6) ◽  
pp. 1974-1977 ◽  
Author(s):  
D. J. Diekema ◽  
S. A. Messer ◽  
R. J. Hollis ◽  
L. B. Boyken ◽  
S. Tendolkar ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Clinical Isolates ◽  
Disk Diffusion ◽  
Broth Microdilution ◽  
Candida Spp

scholarly journals Reliability of the WIDERYST Susceptibility Testing System for Detection of In Vitro Antifungal Resistance in Yeasts

2008 ◽  
Vol 52 (3) ◽  
pp. 1062-1065 ◽  
Author(s):  
Manuel Cuenca-Estrella ◽  
Alicia Gomez-Lopez ◽  
M. Olga Gutierrez ◽  
M. Jose Buitrago ◽  
Juan L. Rodriguez-Tudela
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Clinical Isolates ◽  
Computer Assisted ◽  
Correlation Index ◽  
System A ◽  
Computer Assisted Image Processing ◽  
Computer Assisted Image

ABSTRACT This study evaluated the WIDERYST system, a commercially available computer-assisted image-processing device for the antifungal susceptibility testing of yeasts. A collection of 90 clinical isolates selected to represent ranges of susceptibilities in vitro as broad as possible was tested. An evaluation compared the results obtained by the new system with those achieved by both the Clinical and Laboratory Standards Institute (CLSI) microdilution reference procedure and the antifungal susceptibility standard of the European Committee for Antimicrobial Susceptibility Testing (EUCAST). Overall, the agreement and the correlation index between results obtained by the EUCAST method and the WIDERYST system were 89% and 0.84 (P < 0.01), respectively, and agreement and correlation index between data obtained by the CLSI procedure and the WIDERYST system were 90% and 0.86 (P < 0.01), respectively. The system was able to detect amphotericin B-resistant isolates. All Candida sp. isolates with resistance in vitro to azole agents were detected as well. The system misclassified some isolates belonging to the slowly growing genera Dipodascus and Pichia. A total of 2.7% very major errors were detected for fluconazole. The WIDERYST system is an alternative to reference procedures for antifungal susceptibility testing of clinical isolates of yeasts, particularly for Candida and Cryptococcus species.

Identification and in vitro antifungal susceptibility testing of 200 clinical isolates of Candida spp. responsible for fingernail infections

2007 ◽  
Vol 164 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Valda Teixeira Figueiredo ◽  
Daniel de Assis Santos ◽  
Maria Aparecida Resende ◽  
Júnia Soares Hamdan
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Clinical Isolates ◽  
Candida Spp ◽  
In Vitro Antifungal Susceptibility

Investigation of in vitro antifungal susceptibility testing and genetic diversity of clinical isolates of Trichophyton benhamiae and Trichophyton eriotrephon in Iran

Mycoses ◽  
2020 ◽  
Author(s):  
Saham Ansari ◽  
Bahram Ahmadi ◽  
Mohammad T. Hedayati ◽  
Sadegh Nouripour‐Sisakht ◽  
Mojtaba Taghizadeh‐Armaki ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Clinical Isolates ◽  
Trichophyton Benhamiae ◽  
In Vitro Antifungal Susceptibility

scholarly journals Population differentiation, antifungal susceptibility, and host range of Trichophyton mentagrophytes isolates causing recalcitrant infections in humans and animals

2020 ◽  
Vol 39 (11) ◽  
pp. 2099-2113
Author(s):  
Sebastian Gnat ◽  
Dominik Łagowski ◽  
Aneta Nowakiewicz ◽  
Marcelina Osińska ◽  
Łukasz Kopiński
Keyword(s):  
Host Range ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Trichophyton Mentagrophytes ◽  
Antifungal Susceptibility Testing ◽  
Antifungal Drugs ◽  
Genomic Diversity ◽  
Clinical Isolates ◽  
Chemical Groups

Abstract The major problems in determining the causative factors of the high prevalence of dermatophytoses include the lack of a well-standardized antifungal susceptibility testing method, the low consistency of in vitro and clinical minimal inhibitory concentration values, the high genomic diversity of the population, and the unclear mechanism of pathogenicity. These factors are of particular importance when the disease is recalcitrant and relapses. Herein, we identified and characterized Trichophyton mentagrophytes isolates obtained from therapy-resistant cases in humans and animals. We used genomic diversity analysis of 17 human and 27 animal clinical isolates with the MP-PCR technique, determined their phenotypic enzymatic activity and host range, and performed antifungal susceptibility testing to currently available antifungal drugs from various chemical groups. Genomic diversity values of 35.3% and 33.3% were obtained for clinical isolates from humans and animals, respectively, yet without any relationship to the host species or antifungal drug to which resistance in therapy was revealed. The highest activity of keratinase enzymes was recorded for fox, guinea pig, and human hairs. These hosts can be considered as the main species in the host range of these isolates. A phenyl morpholine derivative, i.e. amorolfine, exhibited superior activity against strains obtained from both humans and animals with the lowest MIC50. Interestingly, high compliance of terbinafine in vitro resistance with clinical problems in the treatment with this substance was shown as well. The high resistance of dermatophytes to drugs is the main cause of the recalcitrance of the infection, whereas the other features of the fungus are less important.

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