Genomic DNA preparation enabling multiple replicate reads for accurate nanopore sequencing
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Sequencing at single-nucleotide resolution using nanopore devices is performed with reported error rates 10.5-20.7% (Ip et al., 2015). Since errors occur randomly during sequencing, repeating the sequencing procedure for the same DNA strands several times can generate sequencing results based on consensus derived from replicate readings, thus reducing overall error rates. The method presented in this manuscript constructs copies of a nucleic acid molecule that are consecutively connected to the nucleic acid molecule. Such copies are useful because they can be sequenced by a nanopore device, enabling replicate reads, thus improving overall sequencing accuracy.
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