Swarming bacterial fronts: Dynamics and morphology of active swarm interfaces propagating through passive frictional domains

Swarming, a multicellular mode of flagella-based motility observed in many bacteria species, enables coordinated and rapid surface translocation, expansion and colonization. In the swarming state, bacterial films display several characteristics of active matter including intense and persistent long-ranged flocks and strong fluctuating velocity fields with significant vorticity. Swarm fronts are typically dynamically evolving interfaces. Many of these fronts separate motile active domains from passive frictional regions comprised of dead or non-motile bacteria. Here, we study the dynamics and structural features of a model active-passive interface in swarming Serratia marcescens. We expose localized regions of the swarm to high intensity wide-spectrum light thereby creating large domains of tightly packed immotile bacteria. When the light source is turned off, swarming bacteria outside this passivated region advance into this highly frictional domain and continuously reshape the interphase boundary. Combining results from Particle Image Velocimetry (PIV) and intensity based image analysis, we find that the evolving interface has quantifiable and defined roughness. Correlations between spatially separated surface fluctuations and damping of the same are influenced by the interaction of the interphase region with adjacently located and emergent collective flows. Dynamical growth exponents characterizing the spatiotemporal features of the surface are extracted and are found to differ from classically expected values for passive growth or erosion. To isolate the effects of hydrodynamic interactions generated by collective flows and that arising from steric interactions, we propose and analyze agent-based simulations with full hydrodynamics of rod-shaped, self-propelled particles. Our computations capture qualitative features of the swarm and predict correlation lengths consistent with experiments. We conclude that hydrodynamic and steric interactions enable different modes of surface dynamics, morphology and thus front invasion.

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Non-Canonical Jets-in-Crossflow

Volume 2: Symposia, Parts A, B, and C ◽

10.1115/fedsm2003-45342 ◽

2003 ◽

Author(s):

Michael W. Plesniak

Keyword(s):

Film Cooling ◽

Particle Image ◽

Vortex Pair ◽

Structural Features ◽

Complementary Information ◽

Global Features ◽

The Past ◽

Jet Trajectory ◽

Image Velocimetry ◽

Counter Rotating Vortex Pair

This invited paper presents a review of research done over the past several years at Purdue on non-canonical jets-in-crossflow. It is a retrospective and an integrative compilation of results previously reported as well as some new ones. The emphasis is on jets emanating from “short” holes, with length-diameter ratios of one or less. These are non-canonical in the sense that the flow is unable to “adjust” to the hole, unlike that in case of a long hole in which fully developed pipe flow can be attained. This motivated by gas turbine film cooling applications. Experimental results acquired with Particle Image Velocimetry will be presented primarily, with some complementary information gained from RANS simulations of the flow. Many different aspects of the problem have been investigated, and in this paper the focus will be on structural features within the hole and in the developing jet and crossflow interaction. A significant result is that the in-hole vertical structures, depending on their sense of rotation, tend to augment or weaken the primary counter-rotating vortex pair. This impacts global features such a jet trajectory and spreading.

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