A Synthetic Genetic Reversible Feynman Gate in a Single E.coli Cell and its Application in Bacterial to Mammalian Cell Information Transfer

AbstractReversible computing is a nonconventional form of computing where the inputs and outputs are mapped in a unique one-to-one fashion. Reversible logic gates in single living cells have not been demonstrated. Here, we created a synthetic genetic reversible Feynman gate in a single E.coli cell. The inputs were extracellular chemicals, IPTG and aTc and the outputs were two fluorescence proteins EGFP and E2-Crimson. We developed a simple mathematical model and simulation to capture the essential features of the genetic Feynman gate and experimentally demonstrated that the behavior of the circuit was ultrasensitive and predictive. We showed an application by creating an intercellular Feynman gate, where input information from bacteria was computed and transferred to HeLa cells through shRNAs delivery and the output signals were observed as silencing of native AKT1 and CTNNB1 genes in HeLa cells. Given that one-to-one input-output mapping, such reversible genetic systems might have applications in diagnostics and sensing, where compositions of multiple input chemicals could be estimated from the outputs.

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Design of Combinational Circuits Using Reversible Decoder in Tanner Tools

Journal of Computational and Theoretical Nanoscience ◽

10.1166/jctn.2020.8436 ◽

2020 ◽

Vol 17 (4) ◽

pp. 1743-1751

Author(s):

R. Kannan ◽

K. Vidhya

Keyword(s):

Low Power ◽

Optical Computing ◽

Logic Gates ◽

Full Adder ◽

Reversible Logic ◽

Quantum Cost ◽

Combinational Circuits ◽

Nano Technology ◽

Feynman Gate ◽

Reversible Logic Gates

Reversible logic is the emerging field for research in present era. The aim of this paper is to realize different types of combinational circuits like full-adder, full-subtractor, multiplexer and comparator using reversible decoder circuit with minimum quantum cost. Reversible decoder is designed using Fredkin gates with minimum Quantum cost. There are many reversible logic gates like Fredkin Gate, Feynman Gate, Double Feynman Gate, Peres Gate, Seynman Gate and many more. Reversible logic is defined as the logic in which the number output lines are equal to the number of input lines i.e., the n-input and k-output Boolean function F(X1,X2,X3, ...,Xn) (referred to as (n,k) function) is said to be reversible if and only if (i) n is equal to k and (ii) each input pattern is mapped uniquely to output pattern. The gate must run forward and backward that is the inputs can also be retrieved from outputs. When the device obeys these two conditions then the second law of thermo-dynamics guarantees that it dissipates no heat. Fan-out and Feed-back are not allowed in Logical Reversibility. Reversible Logic owns its applications in various fields which include Quantum Computing, Optical Computing, Nano-technology, Computer Graphics, low power VLSI etc. Reversible logic is gaining its own importance in recent years largely due to its property of low power consumption. The comparative study in terms of garbage outputs, Quantum Cost, numbers of gates are also presented. The Circuit has been implemented and simulated using Tannaer tools v15.0 software.

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