scholarly journals SOX17 Is Not Required for the Derivation and Maintenance of Mouse Extraembryonic Endoderm Stem Cell Lines

2022 ◽  
Author(s):  
Xudong Dong ◽  
Ailing Ding ◽  
Jiangwei Lin
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Cell Lineage ◽  
Preimplantation Embryos ◽  
Primitive Endoderm ◽  
Extraembryonic Endoderm ◽  
Gene Expression Analyses ◽  
Stem Cell Lines

Extraembryonic endoderm stem (XEN) cell lines can be derived and maintained in vitro and reflect the primitive endoderm cell lineage. SOX17 is thought to be required for the derivation and maintenance of mouse XEN cell lines. Here we have re-evaluated this requirement for SOX17. We derived multiple SOX17-deficient XEN cell lines from preimplantation embryos of a SOX17-Cre knockout strain and chemically converted multiple SOX17-deficient embryonic stem cell lines into XEN cell lines by transient culturing with retinoic acid and Activin A. We confirmed the XEN profile of SOX17-deficient cell lines by immunofluorescence with various markers, by NanoString gene expression analyses, and by their contribution to the extraembryonic endoderm of chimeric embryos produced by injecting these cells into blastocysts. Thus, SOX17 is not required for the derivation and maintenance of XEN cell lines.

scholarly journals The origin of mouse extraembryonic endoderm stem cell lines

2021 ◽  
Author(s):  
Jiangwei Lin
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Cell Lineage ◽  
Preimplantation Embryos ◽  
Visceral Endoderm ◽  
Primitive Endoderm ◽  
Cellular Origin ◽  
Extraembryonic Endoderm ◽  
Parietal Endoderm ◽  
Stem Cell Lines

Mouse extraembryonic endoderm stem (XEN) cell lines can be derived from preimplantation embryos (pre-XEN) and postimplantation embryos (post-XEN). XEN cells share a gene expression profile and cell lineage potential with primitive endoderm (PrE) blastocysts. However, the cellular origin of XEN cells in embryos remains unclear. Here, we report that post-XEN cell lines are derived both from the extraembryonic endoderm and epiblasts of postimplantation embryos and that pre-XEN cell lines are derived both from PrE and epiblasts of blastocysts. Our strategy consisted of deriving post-XEN cells from clumps of epiblasts, parietal endoderm (PE) and visceral endoderm (VE) and deriving pre-XEN cell lines from single PrE and single epiblasts of blastocysts. Thus, XEN cell lines in the mouse embryo originate not only from PrE and PrE-derived lineages but also from epiblast and epiblast-derived lineages of blastocysts and postimplantation embryos.

Derivation and Characterization of Novel Nonhuman Primate Embryonic Stem Cell Lines from In Vitro-Fertilized Baboon Preimplantation Embryos

2011 ◽  
Vol 20 (6) ◽  
pp. 1053-1062 ◽  
Author(s):  
Tien-Cheng Chang ◽  
Ya-Guang Liu ◽  
Carlton A. Eddy ◽  
Ethan S. Jacoby ◽  
Peter A. Binkley ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Nonhuman Primate ◽  
Embryonic Stem ◽  
Preimplantation Embryos ◽  
Stem Cell Lines

scholarly journals Derivation of new human embryonic stem cell lines reveals rapid epigenetic progression in vitro that can be prevented by chemical modification of chromatin

2011 ◽  
Vol 21 (4) ◽  
pp. 751-764 ◽  
Author(s):  
Silvia V. Diaz Perez ◽  
Rachel Kim ◽  
Ziwei Li ◽  
Victor E. Marquez ◽  
Sanjeet Patel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Chemical Modification ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines

Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro

10.1038/74447 ◽  
2000 ◽  
Vol 18 (4) ◽  
pp. 399-404 ◽  
Author(s):  
Benjamin E. Reubinoff ◽  
Martin F. Pera ◽  
Chui-Yee Fong ◽  
Alan Trounson ◽  
Ariff Bongso
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Stem Cell Lines

Chromosomal integrity maintained in five human embryonic stem cell lines after prolonged in vitro culture

2006 ◽  
Vol 14 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Gunilla Caisander ◽  
Hannah Park ◽  
Katarina Frej ◽  
Jenny Lindqvist ◽  
Christina Bergh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
In Vitro Culture ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines

ESTABLISHMENT OF EMBRYONIC STEM CELL LINES DERIVED FROM IN VITRO-PRODUCED BUFFALO (Bubalus bubalis) EMBRYOS

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 186-187
Author(s):  
Vinod Verma ◽  
Sanjeev Gautam ◽  
Manmohansingh Chauhan ◽  
Radheyshyam Manik ◽  
Prabhat Palta ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Bubalus Bubalis ◽  
Stem Cell Lines

Hematopoietic and Endothelial Progenitor Cells from Mouse Adult Testis Derived Stem Cell Lines.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1686-1686
Author(s):  
Jessica Schreiter ◽  
Kaomei Guan ◽  
Karim Nayernia ◽  
Bjoern Chapuy ◽  
Lorenz Truemper ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Germline Stem Cell ◽  
Stem Cell Markers ◽  
Hematopoietic Differentiation ◽  
Stem Cell Lines ◽  
Definitive Hematopoiesis

Abstract Adult mouse testis was recently found to harbour multipotent adult germline stem cells (maGSC) with the differentiation capacity along several mesodermal and epithelial lineages. We here addressed the hematopoietic potential from three multipotent germline stem cell lines in vitro. In the undifferentiated state, these cells expressed stem cell markers including Sca1, Thy1 and c-kit, but lacked markers of definitive hematopoiesis such as CD45. On exposure to in vitro culture conditions favouring hematopoietic differentiation, these cells gave rise to both primitive erythropoiesis with nucleated red blood cells expressing ζ-globin, and definitive hematopoiesis, forming β-globin positive erythroid and myeloid colonies in colony assays. On OP9 stroma cells, the maGSC lines formed mesoderm-like colonies, from which progenitor cell populations for either endothelial or hematopoietic differentiation were isolated. The differentiation patterns and efficiencies were similar to the results obtained from the mouse embryonic stem cell line MPI2. In summary, adult germline derived stem cell lines can be induced to recapitulate early embryonic hemangioblastic development and may thus serve as tools for studies on hematopoietic and angioblastic development as well as applications in regenerative medicine.

scholarly journals Pluripotent pig embryonic stem cell lines originating from in vitro-fertilized and parthenogenetic embryos

2020 ◽  
Vol 49 ◽  
pp. 102093
Author(s):  
Kwang-Hwan Choi ◽  
Dong-Kyung Lee ◽  
Jong-Nam Oh ◽  
Seung-Hun Kim ◽  
Mingyun Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Stem Cell Lines ◽  
Parthenogenetic Embryos
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