scholarly journals Basidiobolus haptosporus-like fungus as a causal agent of gastrointestinal basidiobolomycosis and its link to the common house gecko (Hemidactylus frenatus) as a potential risk factor

2018 ◽  
Author(s):  
Ali Al Bshabshe ◽  
Martin R.P. Joseph ◽  
Ahmed M. Al Hakami ◽  
Tarig Al Azraqi ◽  
Sulieman Al Humayed ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Ribosomal Rna ◽  
Large Subunit ◽  
Causal Agent ◽  
Gut Contents ◽  
Ribosomal Rna Gene ◽  
Source Of Infection ◽  
The Common ◽  
Hemidactylus Frenatus ◽  
Common House

ABSTRACTBasidiobolus spp. are a significant causal agent of infections in man and animals including gastrointestinal basidiobolomycosis (GIB). Little information is available on how these infections are acquired or transmitted, apart from the postulation that environmental sources are implicated. This study aimed to identify Basidiobolus spp. from GIB patients and from the house gecko as a possible source of infection in Aseer, Saudi Arabia. Basidiobolus spp. were isolated from patient specimens (colonic mass biopsy) and from house gecko (gut contents) from Muhayil Aseer areas, in southern Saudi Arabia, using Sabouraud dextrose agar (SDA) which was incubated aerobically for up to three weeks at 30°C. Isolated fungi were initially identified using classical mycological tools and confirmed by sequence analysis of the large subunit ribosomal RNA gene. Cultured specimens from humans and geckos revealed phenotypically similar zygomycete-like fungi which conform to those of Basidiobolus species. The strains formed a monophyletic clade in the 28S ribosomal RNA gene phylogenetic tree. They shared 99.97% similarity with B. haptosporus and 99.97% with B. haptosporus var. minor but have a relatively remote similarity to B. ranarum (99.925%). One isolates from a gecko (L3) fall within the sub-clade encompassing B. haptosporus strain NRRL28635. The study strongly suggests a new and a serious causal agent of GIB related to Basidiobolus haptosporus. The isolation of identical Basidiobolus haptosporus-like strains from humans and lizards from one area is an important step towards identifying risk factors for GIB. Research is underway to screen more environmental niches and fully describe the Basidiobolus strains.

New records of the mites Geckobia bataviensis Vitzthum, 1926 and Pterygosoma dracoensis Jack, 1962 (Trombidiformes: Pterygosomatidae) from Timor-Leste

2021 ◽  
Vol 3 ◽  
pp. ec03041
Author(s):  
Fernando de C. Jacinavicius ◽  
Ricardo Bassini-Silva ◽  
Fabrício H. Oda ◽  
Hinrich Kaiser
Keyword(s):  
New Records ◽  
Timor Leste ◽  
The Common ◽  
Hemidactylus Frenatus ◽  
Common House

We report on the presence of scale mites (Trombidiformes: Pterygosomatidae) parasitizing three species of lizards in Timor-Leste. Geckobia bataviensis Vitzthum, 1926 was found associated with the Common House Gecko, Hemidactylus frenatus Duméril & Bibron, 1836, and the Indopacific Tree Gecko, Hemiphyllodactylus typus Bleeker, 1860, whereas Pterygosoma dracoensis Jack, 1962 was found on the patagia of a Timor Flying Dragon, Draco timoriensis Kuhl, 1820. The association of G. bataviensis with H. frenatus was well documented previously, but this is the first properly documented record of this mite for Timor-Leste. Our report of G. bataviensis on H. typus is the first association of this mite with this gecko. This is the second report of P. dracoensis on D. timoriensis and the first for Timor-Leste.

New observations on Miculenchus Andrássy, 1959 (Nematoda: Tylenchidae) with descriptions of two new and one known species from Iran

Nematology ◽  
2019 ◽  
Vol 21 (9) ◽  
pp. 937-956 ◽  
Author(s):  
Yousef Panahandeh ◽  
Ebrahim Pourjam ◽  
Sergio Álvarez-Ortega ◽  
Farahnaz Jahanshahi Afshar ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Large Subunit ◽  
Small Subunit ◽  
Lsu Rdna ◽  
Natural Forests ◽  
Electron Microscopic ◽  
Pharyngeal Bulb ◽  
Ribosomal Rna Gene ◽  
Molecular Approaches

Summary During nematological surveys in grasslands and natural forests of north and north-western Iran, three species of Miculenchus, including two new and one known species, were recovered and characterised based upon morphological and molecular approaches. Miculenchus brevisalvus n. sp., the first new species, is mainly characterised by its short females 334-388 μm long and with a short 6.0-7.5 μm long stylet, pyriform to pyriform-elongate pharyngeal bulb, 4-8 μm long post-uterine sac (PUS), offset rounded spermatheca filled with small spheroid sperm, elongate conoid tail 62-83 μm long with a sharp tip, and males with simple cloacal lips. Miculenchus muscus n. sp., the second new species, is characterised by a combination of the following features: body 401-467 μm long, well-developed protuberant labial plate at the anterior end under light microscopy, stylet 7-9 μm long, pyriform pharyngeal bulb, PUS 4-9 μm long, gradually narrowing conical tail 62-74 μm long with a finely pointed or sharp end and bearing several fine bristles at tip, and a male with projecting cloacal lips. Both newly described species were morphologically compared with four currently known species of the genus, viz., M. elegans, M. salmae, M. salvus, and M. tesselatus. Miculenchus salmae was also recovered and reported from Iran for the first time. It is mainly characterised by lacking a PUS and the characteristic vagina shape. Miculenchus muscus n. sp. and M. salmae were both characterised using scanning electron microscopic images, yielding new morphological observations for the genus. All three species are studied for their molecular phylogenetic characters using sequences of near-full length fragments of the small subunit ribosomal RNA gene (SSU rDNA) and the D2-D3 expansion segments of the large subunit ribosomal RNA gene (LSU rDNA D2-D3). In both SSU and LSU phylogenies, all currently sequenced species of Miculenchus formed a monophyletic group with maximal clade support in both Bayesian inference and maximum likelihood analysis.

Morphological and phylogenetic analyses reveal a new species of Fistulina (Fistulinaceae, Agaricales) from Australia

Phytotaxa ◽  
2019 ◽  
Vol 420 (3) ◽  
pp. 233-240
Author(s):  
YI-FEI SUN ◽  
SHUN LIU ◽  
BAO-KAI CUI
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Pore Surface ◽  
Ribosomal Rna Gene ◽  
Hyphal System ◽  
Cotton Blue ◽  
A New Species

A new species of Fistulina, F. tasmanica sp. nov., is described and illustrated from Tasmania in Australia. Fistulina tasmanica is distinguished by its pileate and fleshy basidiomata with rose pore surface, fibrous context, separated tubes with small pores (6–7 per mm), a monomitic hyphal system with only clamped generative hyphae, subellipsoid to ellipsoid basidiospores (5.4–6 × 3.4–4 μm) which are negative in Melzer’s reagent and cotton blue. Phylogenetic analyses inferred from the internal transcribed spacer regions (ITS) and the large subunit of nuclear ribosomal RNA gene (nLSU) sequences also confirmed that F. tasmanica is a new member within Fistulina.

Sequence similarities between large subunit ribosomal RNA gene intervening sequences from different Helicobacter species

Gene ◽  
1997 ◽  
Vol 194 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Ana Hurtado ◽  
Jonathan P. Clewley ◽  
Dennis Linton ◽  
Robert J. Owen ◽  
John Stanley
Keyword(s):  
Ribosomal Rna ◽  
Large Subunit ◽  
Ribosomal Rna Gene ◽  
Intervening Sequences ◽  
Sequence Similarities ◽  
Large Subunit Ribosomal Rna
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