scholarly journals The mitochondrial DNA content can not predict the embryo viability

2018 ◽  
Author(s):  
M.S. Yong Qiu ◽  
M.S. Songchang Chen ◽  
Chen Dayang ◽  
M.S. Ping Liu ◽  
M.S. Jun Xia ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Female Patient ◽  
Live Birth ◽  
Dna Content ◽  
Birth Rate ◽  
Live Birth Rate ◽  
Embryo Morphology ◽  
Embryo Viability ◽  
Mitochondrial Dna Content ◽  
Chromosome Status

ABSTRACTObjectiveTo investigate whether the mitochondrial DNA content could predict the embryo viabilityDesignRetrospective analysis.SettingReproductive genetics laboratoryPatient(s)A total of 421 biopsied samples obtained from 129 patientsIntervention(s)Embryo biopsies samples underwent whole genome amplification (WGA) and were tested by next generation sequencing (NGS) and array Comparative Genomic Hybridization (aCGH), 30 samples were selected randomly to undergo quantitative real-time polymerase chain reaction (qPCR).Main Outcome Measure(s)Those embryos which obtained the consistent chromosome status determined both aCGH and NGS platform were further classified. We investigated the relationship of mtDNA content with several factors including female patient age, embryo morphology, chromosome status, and live birth rate of both blastocysts and blastomeres.Result(s)A total of 386 (110 blastomeres and 276 blastocysts) out of 399 embryos showed consistent chromosome status outcome. We found no statistically difference was observed in aneuploid and euploid blastocysts (p=0.14), the same phenomenon was observed in aneuploid and euploid blastomeres (p=0.89). Similarly, the mtDNA content was independent of female patient age, embryo morphology and live birth rate.Conclusion(s)The mtDNA content did not provide a reliable prediction of the viability of blastocysts to initiate a pregnancy.

Utility of the national embryo morphology data collected by SART: correlation between morphologic grade and live birth rate

2009 ◽  
Vol 92 (3) ◽  
pp. S164 ◽  
Author(s):  
M.W. Vernon ◽  
J.E. Stern ◽  
G.D. Ball ◽  
J.D. Wininger ◽  
J.F. Mayer ◽  
...  
Keyword(s):  
Live Birth ◽  
Birth Rate ◽  
Live Birth Rate ◽  
Embryo Morphology

scholarly journals Effect of Age and Embryo Morphology on Live Birth Rate After Transfer of Unbiopsied Blastocysts

2021 ◽  
Author(s):  
Michael Awadalla ◽  
Ashley Kim ◽  
Nicole Vestal ◽  
Jacqueline Ho ◽  
Kristin Bendikson
Keyword(s):  
Live Birth ◽  
Birth Rate ◽  
Live Birth Rate ◽  
Embryo Morphology ◽  
Effect Of Age

scholarly journals ASSOCIATION BETWEEN MITOCHONDRIAL DNA (mtDNA) CONTENT AND LIVE BIRTH RATE (LBR)

2021 ◽  
Vol 116 (3) ◽  
pp. e187
Author(s):  
Irving Olaf Morales ◽  
Roberto Santos Haliscak ◽  
Pasquale Patrizio ◽  
S. Alberto Dávila-Garza ◽  
Genaro Garcia Villafaña
Keyword(s):  
Mitochondrial Dna ◽  
Live Birth ◽  
Birth Rate ◽  
Live Birth Rate

scholarly journals Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer

2021 ◽  
Vol 12 ◽  
Author(s):  
Xuanyou Zhou ◽  
Xueli Liu ◽  
Weihui Shi ◽  
Mujin Ye ◽  
Songchang Chen ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Live Birth ◽  
Embryo Implantation ◽  
Live Birth Rate ◽  
Blastocyst Stage ◽  
Blastocyst Transfer ◽  
Embryo Viability ◽  
Potential Biomarker ◽  
Significant Difference

An increasing number of studies have related the mitochondrial DNA (mtDNA) content to embryo viability and transfer outcomes. However, previous studies have focused more on the relationship between mtDNA and embryo implantation, few studies have studied the effect of the mtDNA content on live birth. In the study, we investigated whether mtDNA content is a reliable screening biomarker for live birth after single blastocyst transfer. A total of 233 couples with 316 blastocyst stage embryos undergoing in vitro fertilization treatment and pre-implantation genetic testing analysis were included in the study. All embryos were chromosomally normal and had undergone single-embryo transfers. There was no significant difference observed in the blastocyst mtDNA content among the live birth, miscarriage and non-implanted groups (p=0.999), and the mtDNA content in blastocysts from the miscarriage and live birth groups was similar [median (interquartile range), 1.00*108(7.59*107- 1.39*108) vs 1.01*108 (7.37*107- 1.32*108)]. Similarly, no significant association was observed between mtDNA content and embryo implantation potential (p=0.965). After adjusting for multiple confounders in a logistic regression analysis with generalized estimating equations, no associations between mtDNA content and live birth were observed in all blastocysts, Day-5 and Day-6 blastocysts (p=0.567, p=0.673, p=0.165, respectively). The live birth rate was not significantly different between blastocysts with an elevated mtDNA content and blastocysts with a normal mtDNA content (26.7% vs 33.6% p=0.780). Additionally, there was no linear correlation between the mtDNA content and maternal age (p=0.570). In conclusion, the mtDNA content does not seem to be a potential biomarker for embryo transfer outcomes (i.e., implantation and live birth) based on the existing testing tools. Embryos with an elevated mtDNA content also have development potential for successful live birth.

Sign in / Sign up

Export Citation Format

Share Document