scholarly journals Engineered orthogonal quorum sensing systems for synthetic gene regulation

2018 ◽  
Author(s):  
Stefan J. Tekel ◽  
Christina L. Smith ◽  
Brianna Lopez ◽  
Amber Mani ◽  
Christopher Connot ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Signaling Pathways ◽  
Gene Networks ◽  
Synthetic Gene ◽  
Synthetic Gene Networks ◽  
Homoserine Lactones ◽  
Signaling Systems ◽  
Sensing Systems ◽  
Engineered Bacteria

AbstractGene regulators that are controlled by membrane-permeable compounds called Homoserine lactones (HSLs) have become popular tools for building synthetic gene networks that coordinate behaviors across populations of engineered bacteria. Synthetic HSL-signaling systems are derived from natural DNA and protein elements from microbial quorum signaling pathways. Crosstalk, where a single HSL can activate multiple regulators, can lead to faults in networks composed of parallel signaling pathways. Here, we report an investigation of quorum sensing components to identify synthetic pathways that exhibit little to no crosstalk in liquid and solid cultures. In previous work, we characterized the response of a single regulator (LuxR) to ten distinct HSL-synthase enzymes. Our current study determined the responses of five different regulators (LuxR, LasR, TraR, BjaR, and AubR) to the same set of synthases. We identified two sets of orthogonal synthase-regulator pairs (BjaI/BjaR + EsaI/TraR and LasI/LasR + EsaI/TraR) that show little to no crosstalk when they are expressed in Escherichia coli BL21. These results expand the toolbox of characterized components for engineering microbial communities.

scholarly journals Two New Plasmid Post-segregational Killing Mechanisms for the Implementation of Synthetic Gene Networks in Escherichia coli

iScience ◽  
2019 ◽  
Vol 14 ◽  
pp. 323-334 ◽  
Author(s):  
Alex J.H. Fedorec ◽  
Tanel Ozdemir ◽  
Anjali Doshi ◽  
Yan-Kay Ho ◽  
Luca Rosa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Networks ◽  
Synthetic Gene ◽  
Synthetic Gene Networks

scholarly journals Engineered Orthogonal Quorum Sensing Systems for Synthetic Gene Regulation in Escherichia coli

2019 ◽  
Vol 7 ◽  
Author(s):  
Stefan J. Tekel ◽  
Christina L. Smith ◽  
Brianna Lopez ◽  
Amber Mani ◽  
Christopher Connot ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Regulation ◽  
Quorum Sensing ◽  
Synthetic Gene ◽  
Sensing Systems

Synthetic gene networks in mammalian cells

2010 ◽  
Vol 21 (5) ◽  
pp. 690-696 ◽  
Author(s):  
Wilfried Weber ◽  
Martin Fussenegger
Keyword(s):  
Mammalian Cells ◽  
Gene Networks ◽  
Synthetic Gene ◽  
Synthetic Gene Networks

Therapeutic synthetic gene networks

2012 ◽  
Vol 23 (5) ◽  
pp. 703-711 ◽  
Author(s):  
Maria Karlsson ◽  
Wilfried Weber
Keyword(s):  
Gene Networks ◽  
Synthetic Gene ◽  
Synthetic Gene Networks

scholarly journals Online model selection for synthetic gene networks

2016 ◽  
Author(s):  
Wei Pan ◽  
Filippo Menolascina ◽  
Guy-Bart Stan
Keyword(s):  
Model Selection ◽  
Gene Networks ◽  
Synthetic Gene ◽  
Synthetic Gene Networks ◽  
Selection For

scholarly journals Quorum-sensing regulation in rhizobia and its role in symbiotic interactions with legumes

2007 ◽  
Vol 362 (1483) ◽  
pp. 1149-1163 ◽  
Author(s):  
Maria Sanchez-Contreras ◽  
Wolfgang D Bauer ◽  
Mengsheng Gao ◽  
Jayne B Robinson ◽  
J Allan Downie
Keyword(s):  
Quorum Sensing ◽  
Potential Role ◽  
Active Role ◽  
Dependent Manner ◽  
Homoserine Lactones ◽  
Signalling Molecules ◽  
Sensing Systems ◽  
Symbiotic Interactions ◽  
Regulatory Systems

Legume-nodulating bacteria (rhizobia) usually produce N -acyl homoserine lactones, which regulate the induction of gene expression in a quorum-sensing (or population-density)-dependent manner. There is significant diversity in the types of quorum-sensing regulatory systems that are present in different rhizobia and no two independent isolates worked on in detail have the same complement of quorum-sensing genes. The genes regulated by quorum sensing appear to be rather diverse and many are associated with adaptive aspects of physiology that are probably important in the rhizosphere. It is evident that some aspects of rhizobial physiology related to the interaction between rhizobia and legumes are influenced by quorum sensing. However, it also appears that the legumes play an active role, both in terms of interfering with the rhizobial quorum-sensing systems and responding to the signalling molecules made by the bacteria. In this article, we review the diversity of quorum-sensing regulation in rhizobia and the potential role of legumes in influencing and responding to this signalling system.

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