Response to a Synthetic Pheromone Source by OX4319L, a Self-Limiting Diamondback Moth (Lepidoptera: Plutellidae) Strain, and Field Dispersal Characteristics of Its Progenitor Strain

2018 ◽  
Author(s):  
Michael Bolton ◽  
Hilda L Collins ◽  
Tracey Chapman ◽  
Neil I Morrison ◽  
Stefan J Long ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Diamondback Moth ◽  
Genetically Engineered ◽  
Field Conditions ◽  
Female Sex Pheromone ◽  
Release Point ◽  
Field Crops ◽  
Synthetic Pheromone ◽  
Mark Release Recapture ◽  
Effective Use

The Diamondback Moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a global pest that infests vegetable and field crops within the Brassica family. A genetically engineered strain of P. xylostella, OX4319L, carrying a self-limiting gene, has shown potential for managing P. xylostella populations, using sustained releases of OX4319L male moths. In order for such a strain to provide control, the transgenic individuals must exhibit attraction to female P. xylostella sex pheromone and adequate dispersal in the field. In this study, we tested these key traits. First, we compared the responses of the OX4319L male moths to a synthetic female sex pheromone source in wind tunnel trials to those of males from three other strains. We found that OX4319L males responded comparably to strains of non-engineered males, with all males flying upwind towards the pheromone source. Second, we used mark-release-recapture studies of a wildtype P. xylostella strain, from which the OX4319L strain was originally developed, to assess dispersal under field conditions. Released males were recaptured using both pheromone-baited and passive traps within a 2.83 ha circular cabbage field, with a recapture rate of 7.93 percent. Males were recaptured up to the boundary of the field at 95 m from the central release point. The median dispersal of males was 14 m. These results showed the progenitor strain of OX4319L retained its ability to disperse within a host field. The results of these experiments are discussed in relation to the potential for the effective use of engineered male-selecting P. xylostella strains under field conditions.

scholarly journals Response to a Synthetic Pheromone Source by OX4319L, a Self-Limiting Diamondback Moth (Lepidoptera: Plutellidae) Strain, and Field Dispersal Characteristics of its Progenitor Strain

2019 ◽  
Vol 112 (4) ◽  
pp. 1546-1551 ◽  
Author(s):  
Michael Bolton ◽  
Hilda L Collins ◽  
Tracey Chapman ◽  
Neil I Morrison ◽  
Stefan J Long ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Diamondback Moth ◽  
Genetically Engineered ◽  
Field Conditions ◽  
Female Sex Pheromone ◽  
Release Point ◽  
Field Crops ◽  
Synthetic Pheromone ◽  
Mark Release Recapture ◽  
Effective Use

AbstractThe diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae), is a global pest that infests vegetable and field crops within the Brassica family. A genetically engineered strain of P. xylostella, OX4319L, carrying a ‘self-limiting’ gene, has shown potential for managing P. xylostella populations, using sustained releases of OX4319L male moths. In order for such a strain to provide control, the transgenic individuals must exhibit attraction to female P. xylostella sex pheromone and adequate dispersal in the field. In this study, we tested these key traits. First, we compared the responses of the OX4319L male moths to a synthetic female sex pheromone source in wind tunnel trials to those of males from three other strains. We found that OX4319L males responded comparably to strains of non-engineered males, with all males flying upwind towards the pheromone source. Second, we used mark-release-recapture studies of a wildtype P. xylostella strain, from which the OX4319L strain was originally developed, to assess dispersal under field conditions. Released males were recaptured using both pheromone-baited and passive traps within a 2.83 ha circular cabbage field, with a recapture rate of 7.93%. Males were recaptured up to the boundary of the field at 95 m from the central release point. The median dispersal of males was 14 m. These results showed the progenitor strain of OX4319L retained its ability to disperse within a host field. The results of these experiments are discussed in relation to the potential for the effective use of engineered male-selecting P. xylostella strains under field conditions.

Female Sex Pheromone of Cameraria ohridella Desch. and Dim. (Lepidoptera: Gracillariidae): Structure Confirmation, Synthesis and Biological Activity of (8E,10Z)-8,10-tetradecadienal and Some Analogues

2002 ◽  
Vol 57 (7-8) ◽  
pp. 739-752 ◽  
Author(s):  
Wittko Francke ◽  
Stephan Franke ◽  
Jan Bergmann ◽  
Till Tolasch ◽  
Mitko Subchev ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Biological Activity ◽  
Sex Pheromone ◽  
Field Tests ◽  
Mass Spectrometric ◽  
Horse Chestnut ◽  
Female Sex Pheromone ◽  
Synthetic Pheromone ◽  
Trap Catches ◽  
Structure Confirmation

Mass spectrometric investigations confirmed the structure of the female produced sex pheromone of the horse-chestnut leafminer Cameraria ohridella Desch. and Dim. to be (8E,10Z)-8,10-tetradecadienal. Pure samples, prepared in a straightforward synthesis, were highly attractive in field tests and proved to be suitable for monitoring of flight activities and population dynamics. In mixtures with the synthetic pheromone, analogues like 9-tridecynal and 7-dodecynyl formate were shown to reduce trap catches. In electroantennographic experiments, pheromone analogues were less active than the pheromone. 9-Tridecynal was the most EAG active analogue tested, followed by 7-dodecyn-1-yl formate and 7-undecyn-1- yl formate.

Identification and field evaluation of female sex pheromone of leaf-eating caterpillar, Opisina arenosella (Lepidoptera: Oecophoridae)

2018 ◽  
Vol 38 (04) ◽  
pp. 274-282
Author(s):  
K. R. M. Bhanu ◽  
D. R. Hall ◽  
R. V. Awalekar ◽  
M. Chandrashekharaiah ◽  
T. N. Divya ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Sex Pheromone ◽  
Coconut Palm ◽  
Field Conditions ◽  
Female Sex Pheromone ◽  
Male Moth ◽  
Opisina Arenosella ◽  
Gland Extract ◽  
Female Pheromone ◽  
Antennal Response

AbstractThe leaf-eating caterpillar,Opisina arenosellaWalker, is the most destructive pest of coconut palm in India and Southeast Asia. The management practices employed againstO. arenosellaso far have been unsuccessful in many instances in India, due to the pest behaviour and coconut palm phenology. The life cycle, incidence and behaviour ofO. arenosellaare rather interesting and useful for the intervention of pheromone trapping technique for its management. We conducted the present study with the intention of identifying the female sex pheromone ofO. arenosellaand testing its efficacy under field conditions. Gas chromatography coupled electroantennographic detection (GC–EAD) and gas chromatography–mass spectrometry (GC–MS) analysis of female pheromone glands extract of one-day-oldO. arenosellafemales confirmed the presence of (Z,Z,Z)-3,6,9-tricosatriene (Z3Z6Z9-23Hy) as the dominant sex pheromone component. The male antennal response to female pheromone gland extract and syntheticZ3Z6Z9-23Hy was recorded using GC–EAD, and the results revealed that antennal response was positive to both the treatments at 0.13 mV and 0.14 mV respectively, compared to control (air), which was 0.016 mV. It was also evident from wind-tunnel experiments that the male moth response was high (80%) with the female gland extract, compared to 60% with synthetic pheromone and 0% for control (air). Male moths caught in the traps with and without lure were assessed in two field sites and recorded 69.26% and 54.25% more moth catches in the traps with the lure. We also observed a similar result in the cage experiment in which male moths caught in the traps with and without lure were 64.50% and 12.40%, respectively. The study also confirmed that 93.20% moths caught in the pheromone-baited traps were male. From the study, it is evident that the presence of (Z,Z,Z)-3,6,9-tricosatriene, which is the sex pheromone compound from the female gland extract ofO. arenosella, is an effective attractant in pheromone traps for the male moth under field conditions.

Studies on the Sex pheromone of the diamondback moth Plutella xylostella (Lepidoptera: Yponomeutidae) in India

1996 ◽  
Vol 86 (5) ◽  
pp. 585-590 ◽  
Author(s):  
G.V.P. Reddy ◽  
K.C.D. Urs
Keyword(s):  
Sex Pheromone ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Virgin Female ◽  
Progressive Reduction ◽  
Synthetic Pheromone ◽  
Synthetic Sex Pheromone

AbstractMarked male moths of Plutella xylostella (Linnaeus) released at up to 12 m from a sticky delta trap baited with synthetic pheromone, were caught in significantly higher numbers than moths released at greater distances, both in downwind and upwind directions, from the traps. Maximum catches were obtained when the traps were set at 30 cm above the crop level. Continued exposure of septa in the field led to a progressive reduction in catches, and catches were significantly reduced after 28 days. Newly emerged virgin female moths attracted significantly more male moths than one day old or older virgin female moths. Five day old virgin females attracted no male moths. When virgin females were used as a bait in the field, the peak catch of males was between 1600 and 1800 h. Tests at two field locations revealed that, at the concentration tested, synthetic sex pheromone attracted a similar number of moths to the number attracted by 10 virgin females in delta traps, and significantly more than 8, or fewer virgin females.

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