scholarly journals Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

2019 ◽  
Author(s):  
Giulia Borile ◽  
Stefano Rossi ◽  
Andrea Filippi ◽  
Enrico Gazzola ◽  
Pietro Capaldo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cell Number ◽  
Label Free ◽  
Cellular Processes ◽  
Spr Sensor ◽  
Microfluidic Chamber ◽  
On Chip

ABSTRACTThe application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.

Label-free, real-time on-chip sensing of living cells via grating-coupled surface plasmon resonance

2019 ◽  
Vol 254 ◽  
pp. 106262 ◽  
Author(s):  
Giulia Borile ◽  
Stefano Rossi ◽  
Andrea Filippi ◽  
Enrico Gazzola ◽  
Pietro Capaldo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Living Cells ◽  
Label Free ◽  
On Chip

A Rapid and Quantitative Serum Test for SARS-CoV-2 Antibodies with Portable Surface Plasmon Resonance Sensing

2020 ◽  
Author(s):  
Abdelhadi Djaileb ◽  
Benjamin Charron ◽  
Maryam Hojjat Jodaylami ◽  
Vincent Thibault ◽  
Julien Coutu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Serum ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
The Novel ◽  
Label Free ◽  
Spr Sensor ◽  
Novel Coronavirus ◽  
Nanomolar Range

We report a surface plasmon resonance (SPR) sensor detecting nucleocapsid antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in undiluted human serum. When exposed to SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the patient population immunized against SARD-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with SARS-CoV-2 nucleocapsid recombinant protein detected anti-SARS-CoV-2 antibodies in the nanomolar range. This bioassay was performed on a portable SPR instrument in undiluted human serum and results were collected within 15 minutes of sample/sensor contact. This strategy paves the way to point-of-care and label-free rapid testing for antibodies.

A Rapid and Quantitative Serum Test for SARS-CoV-2 Antibodies with Portable Surface Plasmon Resonance Sensing

2020 ◽  
Author(s):  
Abdelhadi Djaileb ◽  
Benjamin Charron ◽  
Maryam Hojjat Jodaylami ◽  
Vincent Thibault ◽  
Julien Coutu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Serum ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
The Novel ◽  
Label Free ◽  
Spr Sensor ◽  
Novel Coronavirus ◽  
Nanomolar Range

We report a surface plasmon resonance (SPR) sensor detecting nucleocapsid antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in undiluted human serum. When exposed to SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the patient population immunized against SARD-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with SARS-CoV-2 nucleocapsid recombinant protein detected anti-SARS-CoV-2 antibodies in the nanomolar range. This bioassay was performed on a portable SPR instrument in undiluted human serum and results were collected within 15 minutes of sample/sensor contact. This strategy paves the way to point-of-care and label-free rapid testing for antibodies.

Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays

2007 ◽  
Vol 25 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Rositsa Karamanska ◽  
Jonathan Clarke ◽  
Ola Blixt ◽  
James I. MacRae ◽  
Jiquan Q. Zhang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

scholarly journals Topological insulator overlayer to enhance the sensitivity and detection limit of surface plasmon resonance sensor

Nanophotonics ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1941-1951
Author(s):  
Jiaqi Zhu ◽  
Yuxuan Ke ◽  
Jianfeng Dai ◽  
Qi You ◽  
Leiming Wu ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free ◽  
Spr Sensor ◽  
Wide Range ◽  
Resonance Sensor ◽  
Label Free Detection ◽  
Potential Applications

AbstractSurface plasmon resonance (SPR) sensors have been applied in a wide range of applications for real-time and label-free detection. In this article, by covering the topological insulators nanosheets on the surface of the noble metal (Au), the sensitivity of the SPR sensor is greatly enhanced because of the strong interaction of light with Au–bismuth selenide (Bi2Se3) heterostructure. It is shown that the sensitivity of proposed SPR sensors depends on the concentration of Bi2Se3 solution or the thickness of the coated Bi2Se3 film. The optimised sensitivity (2929.1 nm/RIU) and figure of merit (33.45 RIU−1) have been obtained after three times drop-casting, and the enhancement sensitivity of proposed sensors is up to 51.97% compared to the traditional Au–SPR sensors. Meanwhile, the reflection spectrum is simulated by using the method of effective refractive index, and the reason for the increase of sensitivity is analysed theoretically. For researching the application of modified SPR sensor, heavy metal detection is employed to detect in the last part. Our proposed SPR sensors have potential applications in heavy metal detections and biosensing.

scholarly journals Surface plasmon resonance label-free monitoring of antibody antigen interactions in real time

2007 ◽  
Vol 35 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Asta Kausaite ◽  
Martijn van Dijk ◽  
Jan Castrop ◽  
Almira Ramanaviciene ◽  
John P. Baltrus ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free
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