scholarly journals Phylodynamics of sunflower chlorotic mottle virus, a re-emerging pathosystem

2019 ◽  
Author(s):  
Dariel Cabrera Mederos ◽  
Carolina Torres ◽  
Nicolás Bejerman ◽  
Verónica Trucco ◽  
Sergio Lenardon ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Mottle Virus ◽  
Recent Common Ancestor ◽  
Buenos Aires Province ◽  
Santa Fe ◽  
Dispersal Patterns ◽  
Most Recent Common Ancestor ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Entre Rios

AbstractDistribution and epidemiological patterns of sunflower chlorotic mottle virus (SCMoV) in sunflower (Helianthus annuus L.) growing areas in Argentina were studied from 2006 to 2017. The virus was detected exclusively in the Pampas region (Entre Ríos, Santa Fe, Córdoba, La Pampa and Buenos Aires provinces). Phylodynamic analyses performed using the coat protein gene of SCMoV isolates from sunflower and weeds dated the most recent common ancestor (MRCA) back to 1887 (HPD95% = 1572-1971), which coincides with the dates of sunflower introduction in Argentina. The MRCA was located in the south of Buenos Aires province and was associated with sunflower host (posterior probability for the ancestral host, ppah= 0.98). The Bayesian phylodynamic analyses revealed the dispersal patterns of SCMoV, suggesting a link between natural host diversity, crop displacement by human activities and virus spread.

Dipsacus fullonum: an Alternative Host of Sunflower chlorotic mottle virus in Argentina

2009 ◽  
Vol 157 (5) ◽  
pp. 325-328 ◽  
Author(s):  
Fabián Giolitti ◽  
Nicolás Bejerman ◽  
Sergio Lenardon
Keyword(s):  
Mottle Virus ◽  
Alternative Host ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals The Behaviour of Cowpea Chlorotic Mottle Virus in CsCl

1972 ◽  
Vol 15 (3) ◽  
pp. 247-251 ◽  
Author(s):  
J. B. Bancroft ◽  
I. H. Flack
Keyword(s):  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals In Vitro Synthesis of Cowpea Chlorotic Mottle Virus Polypeptides

1979 ◽  
Vol 44 (2) ◽  
pp. 545-549 ◽  
Author(s):  
J. W. Davies ◽  
B. J. M. Verduin
Keyword(s):  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
In Vitro Synthesis ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals Electrostatic interaction between RNA and protein capsid in cowpea chlorotic mottle virus simulated by a coarse-grain RNA model and a Monte Carlo approach

Biopolymers ◽  
2004 ◽  
Vol 75 (4) ◽  
pp. 325-337 ◽  
Author(s):  
Deqiang Zhang ◽  
Robert Konecny ◽  
Nathan A. Baker ◽  
J. Andrew McCammon
Keyword(s):  
Monte Carlo ◽  
Electrostatic Interaction ◽  
Mottle Virus ◽  
Coarse Grain ◽  
Cowpea Chlorotic Mottle Virus ◽  
Monte Carlo Approach ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Expression and Self Assembly of Cowpea Chlorotic Mottle Virus Capsid Proteins inPichia pastorisand Encapsulation of Fluorescent Myoglobin

2011 ◽  
Vol 1317 ◽  
Author(s):  
Yuanzheng Wu ◽  
Hetong Yang ◽  
Hyun-Jae Shin
Keyword(s):  
Coat Protein ◽  
Large Scale ◽  
Materials Science ◽  
Cesium Chloride ◽  
Size Exclusion ◽  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Abstract:Cowpea chlorotic mottle virus (CCMV) has been a model system for virus studies for over 40 years and now is considered to be a perfect candidate as nanoplatform for applications in materials science and medicine. The ability of CCMV to self assemblein vitrointo virus-like particles (VLPs) or capsids makes an ideal reaction vessel for nanomaterial synthesis and entrapment. Here we report expression of codon optimized CCMV coat protein inPichia pastorisand production of self assembled CCMV VLPs by large-scale fermentation. CCMV coat protein gene (573 bp) was synthesized according to codon preference ofP. pastorisand cloned into pPICZA vector. The recombinant plasmid pPICZA-CP was transformed intoP. pastorisGS115 by electroporation. The resulting yeast colonies were screened by PCR and analyzed for protein expression by SDS-PAGE. After large-scale fermentation CCMV coat protein yields reached 4.8 g L−1. The CCMV VLPs were purified by modified PEG precipitation followed by cesium chloride density gradient ultracentrifugation, and then analyzed by size exclusion fast performance liquid chromatography (FPLC), UV spectrometry and transmission electron microscopy. Myoglobin was used as a model protein to be encapsulated in CCMV VLPs. The fluorescence spectroscopy showed that inclusion of myoglobin had occurred. The results indicated the production of CCMV capsids byP. pastorisfermentation now available for utilization in pharmacology or nanotechnology fields.

Interaction with Capsid Protein Alters RNA Structure and the Pathway for In Vitro Assembly of Cowpea Chlorotic Mottle Virus

2004 ◽  
Vol 335 (2) ◽  
pp. 455-464 ◽  
Author(s):  
Jennifer M. Johnson ◽  
Deborah A. Willits ◽  
Mark J. Young ◽  
Adam Zlotnick
Keyword(s):  
Capsid Protein ◽  
Rna Structure ◽  
Mottle Virus ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
In Vitro Assembly

scholarly journals A Titrimetric and Electrophoretic Study of Cowpea Chlorotic Mottle Virus and its Protein

1973 ◽  
Vol 19 (2) ◽  
pp. 263-273 ◽  
Author(s):  
M. W. Johnson ◽  
G. W. Wagner ◽  
J. B. Bancroft
Keyword(s):  
Mottle Virus ◽  
Electrophoretic Study ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle
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