Imaging Actin in Tissue Slices from Transgenic Mouse Brain

2007 ◽  
Vol 2007 (2) ◽  
pp. pdb.ip28-pdb.ip28
Author(s):  
A. Matus ◽  
V. Biou ◽  
H. Brinkhaus ◽  
M. Roelandse
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Tissue Slices

Conditional control of human wild-type and Parkinson's disease-associated mutant alpha-synuclein in transgenic mouse brain

2004 ◽  
Vol 31 (S 1) ◽  
Author(s):  
S Nuber ◽  
T Schmidt ◽  
D Berg ◽  
M Neumann ◽  
C Holzmann ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Transgenic Mouse ◽  
Mouse Brain ◽  
Alpha Synuclein ◽  
Wild Type ◽  
Conditional Control ◽  
Human Wild Type

Expression of Myoglobin in the Transgenic Mouse Brain

2003 ◽  
pp. 331-345 ◽  
Author(s):  
Ross D. Shonat ◽  
Alan P. Koretsky
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain

scholarly journals Subcellular Localization of Wild-Type and Parkinson's Disease-Associated Mutant α-Synuclein in Human and Transgenic Mouse Brain

2000 ◽  
Vol 20 (17) ◽  
pp. 6365-6373 ◽  
Author(s):  
Philipp J. Kahle ◽  
Manuela Neumann ◽  
Laurence Ozmen ◽  
Veronika Müller ◽  
Helmut Jacobsen ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Subcellular Localization ◽  
Transgenic Mouse ◽  
Mouse Brain ◽  
Wild Type

Specific Uptake of an Amyloid-β Protofibril-Binding Antibody-Tracer in AβPP Transgenic Mouse Brain

2013 ◽  
Vol 37 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Kristina Magnusson ◽  
Dag Sehlin ◽  
Stina Syvänen ◽  
Marie M. Svedberg ◽  
Ola Philipson ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Amyloid Β ◽  
Specific Uptake ◽  
Binding Antibody

scholarly journals Imaging Sterols and Oxysterols in Mouse Brain Reveals Distinct Spatial Cholesterol Metabolism

2018 ◽  
Author(s):  
Eylan Yutuc ◽  
Roberto Angelini ◽  
Mark Baumert ◽  
Natalia Mast ◽  
Irina Pikuleva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Brain Function ◽  
Mass Spectrometry Imaging ◽  
Cholesterol Metabolism ◽  
Biologically Active ◽  
Wild Type ◽  
Tissue Slices ◽  
The Brain

AbstractDysregulated cholesterol metabolism is implicated in a number of neurological disorders. Many sterols, including cholesterol and its precursors and metabolites, are biologically active and important for proper brain function. However, spatial cholesterol metabolism in brain and the resulting sterol distributions are poorly defined. To better understand cholesterol metabolism in situ across the complex functional regions of brain, we have developed on-tissue enzyme-assisted derivatisation in combination with micro-liquid-extraction for surface analysis and liquid chromatography - mass spectrometry to image sterols in tissue slices (10 µm) of mouse brain. The method provides sterolomic analysis at 400 µm spot diameter with a limit of quantification of 0.01 ng/mm2. It overcomes the limitations of previous mass spectrometry imaging techniques in analysis of low abundance and difficult to ionise sterol molecules, allowing isomer differentiation and structure identification. Here we demonstrate the spatial distribution and quantification of multiple sterols involved in cholesterol metabolic pathways in wild type and cholesterol 24S-hydroxylase knock-out mouse brain. The technology described provides a powerful tool for future studies of spatial cholesterol metabolism in healthy and diseased tissues.SignificanceThe brain is a remarkably complex organ and cholesterol homeostasis underpins brain function. It is known that cholesterol is not evenly distributed across different brain regions, however, the precise map of cholesterol metabolism in the brain remains unclear. If cholesterol metabolism is to be correlated with brain function it is essential to generate such a map. Here we describe an advanced mass spectrometry imaging platform to reveal spatial cholesterol metabolism in situ at 400 µm resolution on 10 µm tissue slices from mouse brain. We mapped, not only cholesterol, but also other biologically active sterols arising from cholesterol turnover in both wild type and mice lacking cholesterol 24-hydroxylase (Cyp46a1), the major cholesterol metabolising enzyme.

Age-Associated Inclusions in Normal and Transgenic Mouse Brain

Science ◽  
1992 ◽  
Vol 255 (5050) ◽  
pp. 1445-1445 ◽  
Author(s):  
D. WIRAK ◽  
R. BAYNEY ◽  
T. V. RAMABHADRAN ◽  
R. P. FRACASSO ◽  
J. T. HART ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain

DNA Recombination in the Transgenic Mouse Brain

1993 ◽  
pp. 175-179
Author(s):  
Linda Kingsbury ◽  
Hitoshi Sakano
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Dna Recombination

scholarly journals Cell-specific expression of high levels of human S100 beta in transgenic mouse brain is dependent on gene dosage

1992 ◽  
Vol 12 (11) ◽  
pp. 4337-4346 ◽  
Author(s):  
WC Friend ◽  
S Clapoff ◽  
C Landry ◽  
LE Becker ◽  
D O'Hanlon ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Gene Dosage ◽  
Specific Expression

Clioquinol Reduces Zinc Accumulation in Neuritic Plaques and Inhibits the Amyloidogenic Pathway in AβPP/PS1 Transgenic Mouse Brain

2012 ◽  
Vol 29 (3) ◽  
pp. 549-559 ◽  
Author(s):  
Tao Wang ◽  
Chun-Yan Wang ◽  
Zhong-Yan Shan ◽  
Wei-Ping Teng ◽  
Zhan-You Wang
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Neuritic Plaques ◽  
Zinc Accumulation
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