Measuring Cysteine Cathepsin Activity to Detect Lysosomal Membrane Permeabilization

2016 ◽  
Vol 2016 (5) ◽  
pp. pdb.prot087114 ◽  
Author(s):  
Urška Repnik ◽  
Maruša Hafner Česen ◽  
Boris Turk
Keyword(s):  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Permeabilization ◽  
Cathepsin Activity ◽  
Cysteine Cathepsin

Lysosomal membrane permeabilization as a cell death mechanism in cancer cells

2018 ◽  
Vol 46 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Ana Serrano-Puebla ◽  
Patricia Boya
Keyword(s):  
Cell Death ◽  
Cancer Cells ◽  
Proteolytic Enzymes ◽  
Hydrolytic Enzymes ◽  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Permeabilization ◽  
Regulated Cell Death ◽  
Cathepsin Activity ◽  
Intracellular Cascades

Lysosomes are acidic organelles that contain hydrolytic enzymes that mediate the intracellular degradation of macromolecules. Damage of these organelles often results in lysosomal membrane permeabilization (LMP) and the release into the cytoplasm of the soluble lysosomal contents, which include proteolytic enzymes of the cathepsin family. This, in turn, activates several intracellular cascades that promote a type of regulated cell death, called lysosome-dependent cell death (LDCD). LDCD can be inhibited by pharmacological or genetic blockade of cathepsin activity, or by protecting the lysosomal membrane, thereby stabilizing the organelle. Lysosomal alterations are common in cancer cells and may increase the sensitivity of these cells to agents that promote LMP. In this review, we summarize recent findings supporting the use of LDCD as a means of killing cancer cells.

A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy

2020 ◽  
Author(s):  
Karla Alvarez-Valadez ◽  
Allan Sauvat ◽  
Hélène Fohrer-Ting ◽  
Christophe Klein ◽  
Oliver Kepp ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Permeabilization

scholarly journals Lipotoxicity-mediated cell dysfunction and death involve lysosomal membrane permeabilization and cathepsin L activity

2010 ◽  
Vol 1318 ◽  
pp. 133-143 ◽  
Author(s):  
Frankis G. Almaguel ◽  
Jo-Wen Liu ◽  
Fabio J. Pacheco ◽  
Daisy De Leon ◽  
Carlos A. Casiano ◽  
...  
Keyword(s):  
Cathepsin L ◽  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Permeabilization ◽  
Cell Dysfunction

Visualizing Lysosomal Membrane Permeabilization by Fluorescent Dextran Release: Figure 1.

2015 ◽  
Vol 2015 (10) ◽  
pp. pdb.prot086173 ◽  
Author(s):  
Anne-Marie Ellegaard ◽  
Marja Jäättelä ◽  
Jesper Nylandsted
Keyword(s):  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Permeabilization ◽  
Fluorescent Dextran

Enniatin B1-induced lysosomal membrane permeabilization in mouse embryonic fibroblasts

2019 ◽  
Vol 36 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Carlos A. F. Oliveira ◽  
Lada Ivanova ◽  
Anita Solhaug ◽  
Christiane K. Fæste
Keyword(s):  
Membrane Permeabilization ◽  
Lysosomal Membrane ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Lysosomal Membrane Permeabilization
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