Studies with Escherichia coli Sex Factors

1968 ◽  
Vol 33 (0) ◽  
pp. 425-434 ◽  
Author(s):  
D. Freifelder
Keyword(s):  
Escherichia Coli ◽  
Sex Factors

INCOMPATIBILITY OF INTEGRATED SEX FACTORS IN DOUBLE MALE STRAINS OF ESCHERICHIA COLI

Genetics ◽  
1972 ◽  
Vol 70 (1) ◽  
pp. 31-39
Author(s):  
Anthony R Kaney ◽  
K C Atwood
Keyword(s):  
Escherichia Coli ◽  
Sex Factors ◽  
Chromosome Transfer ◽  
F Factor ◽  
Chromosomal Markers ◽  
K 12

ABSTRACT Several strains of Escherichia coli K-12 harboring two F factors were isolated from Hfr x Hfr crosses. These strains were transiently capable of initiating chromosome transfer from two separate points of origin, and of transferring two different sex factors as integrated chromosomal markers. Each strain tested invariably reverted to a simple Hfr by loss of one of the inherited F factors. The F factor persisting in the revertant was, in nearly every case, that which had been inherited from the recipient Hfr parent.

scholarly journals Transfer characteristics of two resistance determinants in a wild strain of Klebsiella aerogenes (V9A)

1970 ◽  
Vol 16 (2) ◽  
pp. 235-240 ◽  
Author(s):  
E. C. R. Reeve
Keyword(s):  
Escherichia Coli ◽  
Cell Attachment ◽  
Wild Strain ◽  
Klebsiella Aerogenes ◽  
Sex Factors ◽  
Chromosomal Gene ◽  
E Coli ◽  
Resistance Determinants ◽  
A Cell ◽  
Plasmid F

SUMMARYKlebsiella aerogenes strain V9A carries determinants AK and TK, giving resistance to ampicillin and tetracycline, and a plasmid FKlac, but no active sex factor. F− and I-type sex factors were able to transfer TK from V9A to Escherichia coli K12 and between strains of K12, and TK behaved as a separate plasmid with its own replicon. AK could not be transferred, except possibly by a sex factor carrying its own A determinant, but the evidence for such transfer was inconclusive. It is suggested that AK is either a chromosomal gene or is in a plasmid with a cell attachment in Klebsiella not represented in E. coli K12. AK produces a β-lactamase.

Studies on Escherichia coli sex factors

1968 ◽  
Vol 34 (1) ◽  
pp. 31-38 ◽  
Author(s):  
David Freifelder
Keyword(s):  
Escherichia Coli ◽  
Sex Factors

Studies on the chemical nature and size of sex factors of Escherichia coli K12

1963 ◽  
Vol 6 (6) ◽  
pp. 483-497 ◽  
Author(s):  
Patrice J. Driskell-Zamenhof ◽  
Edward A. Adelberg
Keyword(s):  
Escherichia Coli ◽  
Chemical Nature ◽  
Sex Factors ◽  
Escherichia Coli K12

Studies on Escherichia coli sex factors

1968 ◽  
Vol 32 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Dorothy Read Freifelder ◽  
David Freifelder
Keyword(s):  
Escherichia Coli ◽  
Sex Factors

Effective Elimination of Drug Resistance and Sex Factors in Escherichia coli by Sodium Dodecyl Sulfate

1968 ◽  
Vol 95 (3) ◽  
pp. 1078-1089 ◽  
Author(s):  
Munemitsu Tomoeda ◽  
Manabu Inuzuka ◽  
Noriko Kubo ◽  
Sachiko Nakamura
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Sex Factors ◽  
Dodecyl Sulfate

scholarly journals Mutant Hfr strains defective in transfer: restoration by F-like and I-like de-repressed R factors

1970 ◽  
Vol 16 (1) ◽  
pp. 101-112 ◽  
Author(s):  
Marylyn Cooke ◽  
Elinor Meynell ◽  
A. M. Lawn
Keyword(s):  
Escherichia Coli ◽  
Sex Factors ◽  
Serological Tests ◽  
Specific Phage ◽  
F Factor ◽  
Donor Behaviour ◽  
R Factors

SUMMARYMutants of Hfr strains ofEscherichia coliK12 defective in conjugation owing to failure to produce sex pili were isolated by resistance to F-specific phage. Tests of the ability of six de-repressed F-like R factors and four de-repressed I-like R factors to restore Hfr donor behaviour to these mutants indicated that there were several ways in which such restoration could occur. Of a total of 26 defective mutants, 12 were restored by F-like R factors. In these twelve, the function of the integrated F factor was evidently restored, because the sex pili contained F pilin subunits, distinguishable from the R pilin by serological tests. In contrast, among the four I-like R factors, only two were effective, butin all 26 defective mutants; the restored Hfr bacteria produced only I-like pili. The I-like sex factors, in restoring Hfr donor behaviour, did not therefore act by complementing the defective F.

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