Molecular Genetic Analysis of Klebsiella Pneumoniae Nitrogen-fixation (nif) Genes: Appendix: Molecular Genetics of Symbiotic Nitrogen Fixation

1981 ◽  
Vol 45 (0) ◽  
pp. 487-499 ◽  
Author(s):  
G. B. Ruvkun ◽  
S. R. Long ◽  
H. M. Meade ◽  
F. M. Ausubel
Keyword(s):  
Nitrogen Fixation ◽  
Klebsiella Pneumoniae ◽  
Genetic Analysis ◽  
Molecular Genetics ◽  
Symbiotic Nitrogen Fixation ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Nif Genes

Molecular genetics of the parasitic protozoan Leishmania

1995 ◽  
Vol 73 (5-6) ◽  
pp. 235-240 ◽  
Author(s):  
Alexander Kin-Choi Wong
Keyword(s):  
Genetic Analysis ◽  
Molecular Genetics ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Parasitic Protozoan ◽  
The Past ◽  
The Family ◽  
Recent Developments ◽  
Genetic Techniques ◽  
Higher Eukaryotes

Leishmania is a member of the family Trypanosomatidae, which causes debilitating diseases in humans. Over the past several years, researchers have applied molecular genetic techniques to study extensively the biology of this parasitic protozoan. Many aspects of Leishmania biology are found to be unique when compared with the higher eukaryotes. This minireview highlights some recent developments in the molecular genetic analysis of this fascinating organism.Key words: Leishmania, protozoan, molecular genetics, disease.

scholarly journals ANALYSIS OF CANDIDATE POLYMORPHISMS AT EPILEPSY PATIENTS WITHOUT MECHANICAL DISTURBANCES

2018 ◽  
pp. 50-57
Author(s):  
O. Kh. Khamdiyeva ◽  
Zh. B. Tileules ◽  
G. S. Baratzhanova ◽  
A. V. Perfilyeva ◽  
Z. A. Menlimuratova ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Molecular Genetics ◽  
De Novo ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Dravet Syndrome ◽  
De Novo Mutation ◽  
Normal Functioning ◽  
Temporal Epilepsy ◽  
Specific Pcr

Thе article presents the results of a molecular-genetics research on patients with diagnosed epilepsy without mechanical disturbance. The aim of this research was the analysis of candidate gene polymorphisms in development of different forms of epilepsy excepting mechanical reasons. 78 patients of V.M. Savinov SVS clinic with different forms of epilepsy were selected for the molecular-genetic analysis. Genotyping on candidate polimorphisms of gene coding the methyl-СрG-binding protein 2 (МЕСР2, 3 polymorphisms), the genes of sodium (SCN1A, 4 polymorphisms) and potassium (KCNT1, 2 polymorphisms) channels was performed by a site-specific PCR-RFLP method. Molecular genetic analysis revealed the presence of normal functioning alleles for 3 investigated candidate polymorphisms (p.Thr158Met, p.Thr197Met, p.Arg306Ter) of 3d exon of МЕСР2 gene at all epilepsy patients. However, 1 case (patient suffering from Dravet syndrome) of de novo mutation was defined for sodium channel gene (SCN1A p.Ala1783Thr) and 3 cases (2 patients suffering from temporal epilepsy and 1 patient with residual encephalopathy) of new mutations in gene responsible for potassium channel (KCNT1 p.Ala934Thr). To determine the inherited SCN1A and KCNT1 mutations, the molecular-genetics analysis was conducted for close relatives of patients. As a result, we conclude that, candidate polymorphisms of SCN1A p.Ala1783Thr and KCNT1 p.Ala934Thr, disrupting the ion channels normal functioning, can be involved in development of non-mechanical forms of epilepsy.

scholarly journals Repeated molecular genetic analysis in Brugada syndrome revealed a novel disease-associated large deletion in the SCN5A gene

2016 ◽  
Vol 2 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Anders Krogh Broendberg ◽  
Lisbeth Noerum Pedersen ◽  
Jens Cosedis Nielsen ◽  
Henrik Kjaerulf Jensen
Keyword(s):  
Genetic Analysis ◽  
Brugada Syndrome ◽  
Molecular Genetic ◽  
Large Deletion ◽  
Molecular Genetic Analysis ◽  
Scn5a Gene
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