Radiation Scattered from the Plasma Produced by a Focused Ruby Laser Beam

With pinacyanol as the supravital stain, a preferential effect on mitochondria of KB cells was achieved by the irradiation with the ruby laser beam. The observation confirmed the results of other workers using janus green B in the same experimental system. The preferential effect on mitochondria was noted in the area extending 8–10 µ beyond the nonpreferential damage of 4–5 µ in diameter. The opaque material associated with mitochondria possibly represented coagulated protein. The effect involved cristae mitochondriales without severe disarrangement of their structure. The opaque material could be interpreted as the result of direct interaction between mitochondria and the laser beam, even though the mitochondria were noted outside of the previously estimated focal spot size of about 3 µ Within the thickness of 2–4 µ of monolayered cells, larger areas of damage can be accounted for by divergence of the beam which is focused by a microscope objective of very short focal length. A threshold of biologic effectiveness is probably also involved.

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Evidence for a Thermonuclear Reaction in aθ-Pinch Plasma from the Scattering of a Ruby Laser Beam

Physical Review Letters ◽

10.1103/physrevlett.19.688 ◽

1967 ◽

Vol 19 (12) ◽

pp. 688-689 ◽

Cited By ~ 13

Author(s):

S. A. Ramsden ◽

P. K. John ◽

B. Kronast ◽

R. Benesch

Keyword(s):

Laser Beam ◽

Ruby Laser ◽

Thermonuclear Reaction ◽

Pinch Plasma

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Spatial coherence of a ruby laser beam by interferometry

Applied Optics ◽

10.1364/ao.17.003500 ◽

1978 ◽

Vol 17 (21) ◽

pp. 3500 ◽

Cited By ~ 1

Author(s):

H. Madjidi-Zolbanine

Keyword(s):

Laser Beam ◽

Ruby Laser ◽

Spatial Coherence

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Manyframe High Speed Schlieren Photography of Shock Waves in a Ruby Laser Beam

High Speed Photography ◽

10.1007/978-1-4613-3377-7_104 ◽

1975 ◽

pp. 616-620 ◽

Cited By ~ 1

Author(s):

A. S. Shikanov ◽

Yu. A. Zakharenkov

Keyword(s):

Shock Waves ◽

Laser Beam ◽

High Speed ◽

Ruby Laser ◽

Schlieren Photography

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Scattering of Ruby-Laser Beam by Gases

Physical Review Letters ◽

10.1103/physrevlett.11.403 ◽

1963 ◽

Vol 11 (9) ◽

pp. 403-406 ◽

Cited By ~ 20

Author(s):

T. V. George ◽

L. Slama ◽

M. Yokoyama ◽

L. Goldstein

Keyword(s):

Laser Beam ◽

Ruby Laser

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Self-bending of a ruby laser beam in CdSxSe1–xsemiconductor crystals

Soviet Journal of Quantum Electronics ◽

10.1070/qe1975v005n03abeh011015 ◽

1975 ◽

Vol 5 (3) ◽

pp. 340-342 ◽

Cited By ~ 6

Author(s):

A A Borshch ◽

M S Brodin ◽

V I Volkov ◽

V V Ovchar

Keyword(s):

Laser Beam ◽

Ruby Laser

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LASER MICROSCOPE IRRADIATION OF PHYSARUM POLYCEPHALUM: DYNAMIC AND ULTRASTRUCTURAL EFFECTS

The Journal of Cell Biology ◽

10.1083/jcb.40.1.108 ◽

1969 ◽

Vol 40 (1) ◽

pp. 108-119 ◽

Cited By ~ 13

Author(s):

Joe L. Griffin ◽

Marvin N. Stein ◽

Robert E. Stowell

Keyword(s):

Electron Microscopy ◽

Laser Beam ◽

Incident Energy ◽

Physarum Polycephalum ◽

Ruby Laser ◽

Movement Patterns ◽

Transient Responses ◽

Pigment Granules

Streaming plasmodia of Physarum polycephalum were irradiated with a microscope-mounted ruby laser and the resulting changes were recorded by cinemicrography or streak photographs. Some lesions were processed for electron microscopy. By varying the incident energy, three levels of response were detected. Two transient responses, a gelation briefly blocking streams and a more severe gelation with contraction, changed movement patterns but not organelle ultrastructure. At higher energies, a permanently coagulated lesion was rapidly segregated from normal and transiently altered cytoplasm by formation of new membranes. Within the coagulum, pigment granules were destroyed, membranes were disrupted, and cytoplasm was flocculent. Nuclei and mitochondria were compact in the center and swollen in a peripheral space left by contraction of the coagulum. These changes are probably caused by heat produced by the interaction between the laser beam and the pigment granules of the plasmodium. Many of the changes seem to be secondary responses that follow the primary capture of energy during irradiation.

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