scholarly journals Factors Influencing Spore Germination and Early Gametophyte Development in Anemia mexicana and Anemia phyllitidis

1986 ◽  
Vol 82 (1) ◽  
pp. 230-235 ◽  
Author(s):  
Joan E. Nester ◽  
Ronald C. Coolbaugh
1991 ◽  
Vol 19 (3) ◽  
pp. 308-315
Author(s):  
Martin E. Bannon ◽  
Herbert A. Kordan ◽  
Elizabeth Sheffield

Spores from the ferns Pteridium aquilinum and Anemia phyllitidis were germinated on solid Moore's medium, supplemented with the three oxybarbiturates, namely, barbital, amobarbital and secobarbital, under white light. The severity of the adverse action of these neuroactive agents on spore germination, and in most instances, on subsequent gametophyte development, was secobarbital>amobarbital>barbital. A. phyllitidis was, on the whole, more sensitive than P. aquilinum to the adverse effects exerted by the three oxybarbiturates on spore germination and subsequent gametophyte development.


1968 ◽  
Vol 14 (5) ◽  
pp. 579-585 ◽  
Author(s):  
K. P. Klatt ◽  
J. E. Gander

The uptake of tartrate by P. charlesii has been studied for cultures that were either aerated by shaking or were kept stationary. Stationary cultures were permeable to tartrate when high concentrations of NH4+ (above 36 mM) and glucose (278 mM) were present. Manganous ion (10−5 M) was required for the uptake of tartrate by stationary cultures containing high concentrations of NH4+. Both stationary and shake cultures were able to remove tartrate from the medium when the glucose concentration was reduced below 278 mM; the process was then no longer dependent upon the presence of Mn2+. The influence of changes in the concentrations of glucose and NH4+ was not related to the biochemical events of spore germination.


2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Yupi ISNAINI ◽  
Titien Ngatinem Praptosuwiryo

Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and α-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.


2003 ◽  
Vol 81 (5) ◽  
pp. 501-516 ◽  
Author(s):  
Aniceto Mendoza-Ruiz ◽  
Blanca Pérez-García

A comparative analysis is presented of the spore morphology, germination pattern, and prothallial development of the sexual phase of seven species in the fern genus Phanerophlebia C. Presl. Gametophyte development was studied from samples grown both on agar and soil. Spores are monolete, ellipsoid, with light brown to dark brown perine; the germination pattern is Vittaria-type and the prothallial development is Aspidium-type in all of the species. The gametangia are of the type typical for leptosporangiate ferns. Gametophytes of all species initially become female, then bisexual. Differences among species include spore germination time (6–12 days), shape of the gametophytes (spatulate–cordiform to cordiform–reniform, with smooth to very irregular margins), development time of trichomes (12–24 days), and appearance of gametangia (40–200 days). Some species develop the first leaves of the sporophytes after 200 days. Results are contrasted with previously published reports on gametophyte development in Arachniodes Blume, Cyrtomium C. Presl, Didymochlaena Desv., Dryopteris Adans, Olfersia Raddi, Polystichum Roth, and Stigmatopteris C. Chr.Key words: Dryopteridaceae, fern gametophyte, Mexico, morphogenesis, Phanerophlebia, sexual phase.


Author(s):  
V. Raghavan

SynopsisCertain aspects of the cytology of germination of spores of Anemia phyllitidis and Pteris vittata, with particular reference to the role of nucleic acid synthesis, are reviewed. As studied by autoradiography of 3H-thymidine or 3H-uridine incorporation, spore germination resulting in the formation of the rhizoid and protonemal cell is accompanied by the synthesis of DNA and RNA. Experiments using inhibitors of RNA synthesis on gibberellic acid-induced germination of spores of A. phyllitidis have indicated that dry spores contain conserved mRNA and that gibberellic acid-induced polyadenylation of mRNA occurs during the early phase of germination of spores. Recent demonstrations of the presence of polyadenylated mRNA in dry spores of A. phyllitidis suggest specific roles for both functional, conserved mRNA containing polyadenylic acid, as well as for gibberellic acid-induced polyadenylation of mRNA during the early phase of spore germination.


2012 ◽  
Vol 102 (3) ◽  
pp. 208-215 ◽  
Author(s):  
K. M. Zhang ◽  
J. H. Liu ◽  
X. Cheng ◽  
G. F. Zhang ◽  
Y. M. Fang ◽  
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