Comparative analysis of the sexual phase of Phanerophlebia (Dryopteridaceae) in Mexico

2003 ◽  
Vol 81 (5) ◽  
pp. 501-516 ◽  
Author(s):  
Aniceto Mendoza-Ruiz ◽  
Blanca Pérez-García
Keyword(s):  
Comparative Analysis ◽  
Spore Germination ◽  
Development Time ◽  
Spore Morphology ◽  
Fern Gametophyte ◽  
Gametophyte Development ◽  
Germination Time

A comparative analysis is presented of the spore morphology, germination pattern, and prothallial development of the sexual phase of seven species in the fern genus Phanerophlebia C. Presl. Gametophyte development was studied from samples grown both on agar and soil. Spores are monolete, ellipsoid, with light brown to dark brown perine; the germination pattern is Vittaria-type and the prothallial development is Aspidium-type in all of the species. The gametangia are of the type typical for leptosporangiate ferns. Gametophytes of all species initially become female, then bisexual. Differences among species include spore germination time (6–12 days), shape of the gametophytes (spatulate–cordiform to cordiform–reniform, with smooth to very irregular margins), development time of trichomes (12–24 days), and appearance of gametangia (40–200 days). Some species develop the first leaves of the sporophytes after 200 days. Results are contrasted with previously published reports on gametophyte development in Arachniodes Blume, Cyrtomium C. Presl, Didymochlaena Desv., Dryopteris Adans, Olfersia Raddi, Polystichum Roth, and Stigmatopteris C. Chr.Key words: Dryopteridaceae, fern gametophyte, Mexico, morphogenesis, Phanerophlebia, sexual phase.

scholarly journals In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Yupi ISNAINI ◽  
Titien Ngatinem Praptosuwiryo
Keyword(s):  
Spore Germination ◽  
Culture Media ◽  
Basal Medium ◽  
Modern Medicine ◽  
Ex Situ ◽  
Naphthalene Acetic Acid ◽  
Gametophyte Development ◽  
In The Wild ◽  
Rare And Endangered

Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and α-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.

scholarly journals Palynological analysis of Dennstaedtiaceae taxa from the Paranaense Phytogeographic Province that produce Trilete spores II: Microlepia speluncae and Pteridium arachnoideum

2016 ◽  
Vol 88 (2) ◽  
pp. 877-890 ◽  
Author(s):  
Agustina Yañez ◽  
Gonzalo J. Marquez ◽  
Marta A. Morbelli
Keyword(s):  
Comparative Analysis ◽  
Three Dimensional ◽  
Middle Layer ◽  
The Other ◽  
Spore Morphology ◽  
Central Channel ◽  
Palynological Analysis ◽  
Dimensional Network ◽  
The Family ◽  
Wall Ultrastructure

The spore morphology and wall ultrastructure of Microlepia speluncae and Pteridium arachnoideum from the Paranaense Province were analyzed with LM, SEM and TEM and a comparative analysis was carried out. In both species the spores are covered by a three-dimensional network of threads branched and fused, tangentially arranged to the surface, and some free-end threads are also seen. The species were differentiated by morphology and the frequency of threads fusion and the networks distribution on the surface of the spores. In both species the exospore is two-layered in section, both layers are traversed by single or branched channels. The perispore is three-layered in section: the inner layer is adhered to the exospore, the middle layer is formed of a three-dimensional network of threads and the outer layer is discontinuous. The perispore ultrastructure of Microlepia speluncae was interpreted as formed of helical subunits displayed around a central channel. The spore morphology and perispore ultrastructure allow differentiating Microlepia from Pteridium but also to the other Dennstaedtiaceae genera that grow in the Paranaense Province. The results obtained allow establishing relationships that let us recognize different groups and gave a new reference to get a better knowledge of the family.

scholarly journals The influence of temperature and humidity on spore formation and germination inBacillus anthracis

1960 ◽  
Vol 58 (2) ◽  
pp. 177-186 ◽  
Author(s):  
D. G. Davies
Keyword(s):  
Relative Humidity ◽  
Spore Germination ◽  
Spore Formation ◽  
Influence Of Temperature ◽  
Germination Time ◽  
Temperature Range ◽  
Wide Range ◽  
Temperature And Humidity ◽  
Formed Part ◽  
Lower Temperature

Experiments performed on spore formation inB. anthracis, in a wide range of closed atmospheres of chemically controlled humidity, showed that the sporing time ofB. anthracisis prolonged considerably as the humidity decreases, both at 26 and 37° C. The effect is less noticeable at the lower temperature.Experiments performed on spore germination in the same organism showed that a temperature range of 20–44° C. permitted germination of the five strains tested. Above a temperature of 39° C. and below 30° C. germination time lengthened considerably.Experiments performed on the influence of humidity on spore germination, both at 26 and 37° C. showed that no germination could take place at a relative humidity of 80% or below. Germination time was considerably prolonged when the humidity value fell below 100%.This work formed part of a thesis for the degree of M.D. in Bristol University. I wish to thank Dr G. S. Wilson for his advice in preparing this paper.

scholarly journals Effects of Ageratina adenophora on Spore Germination and Gametophyte Development of Neocheiropteris palmatopedata

2012 ◽  
Vol 102 (3) ◽  
pp. 208-215 ◽  
Author(s):  
K. M. Zhang ◽  
J. H. Liu ◽  
X. Cheng ◽  
G. F. Zhang ◽  
Y. M. Fang ◽  
...  
Keyword(s):  
Spore Germination ◽  
Gametophyte Development ◽  
Ageratina Adenophora
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