Research on Biological Materials for Artificial Propagation of Bird Nest Fern Spore

Bird Nest Fern is a perennial evergreen large epiphytic or herb. Under natural conditions, most nest fern spores lack of suitable conditions for germination, and because of certain conditions are not met and stop growth. the people digging improperly on the Nest Fern ,the Nest Fern resources have been severely damaged, in order to protect the Nest Fern natural resources .we should strengthen the cultivation of nest fern. This study were different amount of coconut husk, different concentrations of 6-BA and different concentrations of GA3 nest fern spore germination test, results are as follows:When the Coconut husk amount of certain nest fern spore germination rate increased with increasing concentration of 6-BA,6-BA concentration of 0.7mg/L,the spore germination rate of 6.25%. Concentration of 6-BA, nest fern spore germination rate increased with the coconut husk increased. When the coconut husk volume and 6-BA concentration must soak spores with different concentrations of GA3 12~15min, nest fern spore germination with GA3 concentration increases, by analysis of variance, GA3 concentration of 200 mg/L, can greatly enhance spore germination rate; Three true leaves of seedlings grown by transplanting, and has successfully bred a number of nest fern seedlings.

Effects of Oxybarbiturates on Fern Spore Germination and Gametophyte Development

Spores from the ferns Pteridium aquilinum and Anemia phyllitidis were germinated on solid Moore's medium, supplemented with the three oxybarbiturates, namely, barbital, amobarbital and secobarbital, under white light. The severity of the adverse action of these neuroactive agents on spore germination, and in most instances, on subsequent gametophyte development, was secobarbital>amobarbital>barbital. A. phyllitidis was, on the whole, more sensitive than P. aquilinum to the adverse effects exerted by the three oxybarbiturates on spore germination and subsequent gametophyte development.