scholarly journals Target Site Specificity of the Tos17 Retrotransposon Shows a Preference for Insertion within Genes and against Insertion in Retrotransposon-Rich Regions of the Genome

2003 ◽  
Vol 15 (8) ◽  
pp. 1771-1780 ◽  
Author(s):  
Akio Miyao ◽  
Katsuyuki Tanaka ◽  
Kazumasa Murata ◽  
Hiromichi Sawaki ◽  
Shin Takeda ◽  
...  
Keyword(s):  
Target Site ◽  
Site Specificity

scholarly journals Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis

2019 ◽  
Vol 39 (13) ◽  
Author(s):  
Angad Garg ◽  
Yehuda Goldgur ◽  
Ana M. Sanchez ◽  
Beate Schwer ◽  
Stewart Shuman
Keyword(s):  
Dna Binding ◽  
Fission Yeast ◽  
Dna Sequences ◽  
Phosphate Starvation ◽  
Transcriptional Factor ◽  
Target Site ◽  
Site Specificity ◽  
Phosphate Homeostasis ◽  
Content Type ◽  
Promoter Dna

ABSTRACT Pho7 is the Schizosaccharomyces pombe fission yeast Zn2Cys6 transcriptional factor that drives a response to phosphate starvation in which phosphate acquisition genes are upregulated. Here we report a crystal structure at 1.6-Å resolution of the Pho7 DNA-binding domain (DBD) bound at its target site 2 in the pho1 promoter (5′-TCGGAAATTAAAAA). Comparison to the previously reported structure of Pho7 DBD in complex with its binding site in the tgp1 promoter (5′-TCGGACATTCAAAT) reveals shared determinants of target site specificity as well as variations in the protein-DNA interface that accommodate different promoter DNA sequences. Mutagenesis of Pho7 amino acids at the DNA interface identified nucleobase contacts at the periphery of the footprint that are essential for the induction of pho1 expression in response to phosphate starvation and for Pho7 binding to site 1 in the pho1 promoter.

scholarly journals Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity

2019 ◽  
Vol 76 (3) ◽  
pp. 423-436.e3 ◽  
Author(s):  
Jonas Westergaard Højfeldt ◽  
Lin Hedehus ◽  
Anne Laugesen ◽  
Tülin Tatar ◽  
Laura Wiehle ◽  
...  
Keyword(s):  
Target Site ◽  
Site Specificity

scholarly journals Structural Basis for the Activation and Target Site Specificity of CDC7 Kinase

Structure ◽  
2020 ◽  
Vol 28 (8) ◽  
pp. 954-962.e4 ◽  
Author(s):  
Samual D. Dick ◽  
Stefania Federico ◽  
Siobhan M. Hughes ◽  
Valerie E. Pye ◽  
Nicola O'Reilly ◽  
...  
Keyword(s):  
Target Site ◽  
Structural Basis ◽  
Site Specificity ◽  
Cdc7 Kinase

scholarly journals Functional Characterization of Tn4401, a Tn3-Based Transposon Involved inblaKPCGene Mobilization

2011 ◽  
Vol 55 (11) ◽  
pp. 5370-5373 ◽  
Author(s):  
Gaelle Cuzon ◽  
Thierry Naas ◽  
Patrice Nordmann
Keyword(s):  
High Frequency ◽  
Recipient Cell ◽  
Functional Characterization ◽  
Target Site ◽  
Target Site Duplication ◽  
Site Specificity ◽  
Carbapenemase Gene

ABSTRACTThe carbapenemase geneblaKPC, which is rapidly spreading worldwide, is located on a Tn3-based transposon, Tn4401. In a transposition-conjugation assay, Tn4401was able to mobilizeblaKPC-2gene at a frequency of 4.4 × 10−6/recipient cell. A 5-bp target site duplication was evidenced upon each insertion without target site specificity. This study demonstrated that Tn4401is an active transposon capable of mobilizingblaKPCgenes at high frequency.

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