Coexpression, purification, crystallization and preliminary X-ray characterization of glycine decarboxylase (P-protein) of the glycine-cleavage system fromThermus thermophilusHB8

2003 ◽  
Vol 59 (3) ◽  
pp. 554-557 ◽  
Author(s):  
Tadashi Nakai ◽  
Noriko Nakagawa ◽  
Nobuko Maoka ◽  
Ryoji Masui ◽  
Seiki Kuramitsu ◽  
...  
Keyword(s):  
Glycine Decarboxylase ◽  
Glycine Cleavage System ◽  
X Ray ◽  
P Protein ◽  
Glycine Cleavage

scholarly journals Synthesis and Characterization of Selenolipoylated H-protein of the Glycine Cleavage System

1997 ◽  
Vol 272 (32) ◽  
pp. 19880-19883 ◽  
Author(s):  
Kazuko Fujiwara ◽  
Kazuko Okamura-Ikeda ◽  
Lester Packer ◽  
Yutaro Motokawa
Keyword(s):  
Synthesis And Characterization ◽  
Glycine Cleavage System ◽  
Glycine Cleavage ◽  
H Protein

scholarly journals Effects of tetrahydrofolate polyglutamates on the kinetic parameters of serine hydroxymethyltransferase and glycine decarboxylase from pea leaf mitochondria

1993 ◽  
Vol 292 (2) ◽  
pp. 425-430 ◽  
Author(s):  
V Besson ◽  
F Rebeille ◽  
M Neuburger ◽  
R Douce ◽  
E A Cossins
Keyword(s):  
Kinetic Parameters ◽  
Serine Hydroxymethyltransferase ◽  
Affinity Constant ◽  
Plant Tissues ◽  
Glycine Decarboxylase ◽  
Glycine Cleavage System ◽  
Matrix Space ◽  
The Matrix ◽  
Order Of Magnitude ◽  
Glycine Cleavage

Plant tissues contain highly conjugated forms of folate. Despite this, the ability of plant folate-dependent enzymes to utilize tetrahydrofolate polyglutamates has not been examined in detail. In leaf mitochondria, the glycine-cleavage system and serine hydroxymethyltransferase, present in large amounts in the matrix space and involved in the photorespiratory cycle, necessitate the presence of tetrahydrofolate as a cofactor. The aim of the present work was to determine whether glutamate chain length (one to six glutamate residues) influenced the affinity constant for tetrahydrofolate and the maximal velocities displayed by these two enzymes. The results show that the affinity constant decreased by at least one order of magnitude when the tetrahydrofolate substrate contained three or more glutamate residues. In contrast, maximal velocities were not altered in the presence of these substrates. These results are consistent with analyses of mitochondrial folates which revealed a pool of polyglutamates dominated by tetra and pentaglutamates. The equilibrium constant of the serine hydroxymethyltransferase suggests that, during photorespiration, the reaction must be permanently pushed toward the formation of serine (the unfavourable direction) to allow the recycling of tetrahydrofolate necessary for the operation of the glycine decarboxylase T-protein.

scholarly journals High-Resolution X-ray Crystallography Studies of the H-Protein of Glycine Cleavage System

2008 ◽  
Vol S2 (01) ◽  
pp. 286-286
Author(s):  
A. Nakagawa ◽  
A. Higashiura ◽  
T. Kurakane ◽  
M. Matsuda ◽  
M. Suzuki ◽  
...  
Keyword(s):  
High Resolution ◽  
Glycine Cleavage System ◽  
X Ray ◽  
X Ray Crystallography ◽  
Glycine Cleavage ◽  
H Protein

scholarly journals Crystal structure of P-protein of the glycine cleavage system

2005 ◽  
Vol 61 (a1) ◽  
pp. c189-c190
Author(s):  
T. Nakai ◽  
N. Nakagawa ◽  
N. Maoka ◽  
R. Masui ◽  
S. Kuramitsu ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Glycine Cleavage System ◽  
P Protein ◽  
Glycine Cleavage
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