Biomagnetic Imaging: A View From Fourier Space

2005 ◽  
Author(s):  
W.J. Dallas
Author(s):  
S. Cusack ◽  
J.-C. Jésior

Three-dimensional reconstruction techniques using electron microscopy have been principally developed for application to 2-D arrays (i.e. monolayers) of biological molecules and symmetrical single particles (e.g. helical viruses). However many biological molecules that crystallise form multilayered microcrystals which are unsuitable for study by either the standard methods of 3-D reconstruction or, because of their size, by X-ray crystallography. The grid sectioning technique enables a number of different projections of such microcrystals to be obtained in well defined directions (e.g. parallel to crystal axes) and poses the problem of how best these projections can be used to reconstruct the packing and shape of the molecules forming the microcrystal.Given sufficient projections there may be enough information to do a crystallographic reconstruction in Fourier space. We however have considered the situation where only a limited number of projections are available, as for example in the case of catalase platelets where three orthogonal and two diagonal projections have been obtained (Fig. 1).


Author(s):  
E. Voelkl ◽  
L. F. Allard

The conventional discrete Fourier transform can be extended to a discrete Extended Fourier transform (EFT). The EFT allows to work with discrete data in close analogy to the optical bench, where continuous data are processed. The EFT includes a capability to increase or decrease the resolution in Fourier space (thus the argument that CCD cameras with a higher number of pixels to increase the resolution in Fourier space is no longer valid). Fourier transforms may also be shifted with arbitrary increments, which is important in electron holography. Still, the analogy between the optical bench and discrete optics on a computer is limited by the Nyquist limit. In this abstract we discuss the capability with the EFT to change the initial sampling rate si of a recorded or simulated image to any other(final) sampling rate sf.


Author(s):  
Neng-Yu Zhang ◽  
Bruce F. McEwen ◽  
Joachim Frank

Reconstructions of asymmetric objects computed by electron tomography are distorted due to the absence of information, usually in an angular range from 60 to 90°, which produces a “missing wedge” in Fourier space. These distortions often interfere with the interpretation of results and thus limit biological ultrastructural information which can be obtained. We have attempted to use the Method of Projections Onto Convex Sets (POCS) for restoring the missing information. In POCS, use is made of the fact that known constraints such as positivity, spatial boundedness or an upper energy bound define convex sets in function space. Enforcement of such constraints takes place by iterating a sequence of function-space projections, starting from the original reconstruction, onto the convex sets, until a function in the intersection of all sets is found. First applications of this technique in the field of electron microscopy have been promising.To test POCS on experimental data, we have artificially reduced the range of an existing projection set of a selectively stained Golgi apparatus from ±60° to ±50°, and computed the reconstruction from the reduced set (51 projections). The specimen was prepared from a bull frog spinal ganglion as described by Lindsey and Ellisman and imaged in the high-voltage electron microscope.


2008 ◽  
Vol 12 (2) ◽  
pp. 1-29 ◽  
Author(s):  
Kenneth Jackson ◽  
Sebastian Jaimungal ◽  
Vladimir Surkov

2021 ◽  
Vol 504 (1) ◽  
pp. 33-52
Author(s):  
Gong-Bo Zhao ◽  
Yuting Wang ◽  
Atsushi Taruya ◽  
Weibing Zhang ◽  
Héctor Gil-Marín ◽  
...  

ABSTRACT We perform a joint BAO and RSD analysis using the eBOSS DR16 LRG and ELG samples in the redshift range of z ∈ [0.6, 1.1], and detect an RSD signal from the cross-power spectrum at a ∼4σ confidence level, i.e., fσ8 = 0.317 ± 0.080 at zeff = 0.77. Based on the chained power spectrum, which is a new development in this work to mitigate the angular systematics, we measure the BAO distances and growth rate simultaneously at two effective redshifts, namely, DM/rd (z = 0.70) = 17.96 ± 0.51, DH/rd (z = 0.70) = 21.22 ± 1.20, fσ8 (z = 0.70) = 0.43 ± 0.05, and DM/rd (z = 0.845) = 18.90 ± 0.78, DH/rd (z = 0.845) = 20.91 ± 2.86, fσ8 (z = 0.845) = 0.30 ± 0.08. Combined with BAO measurements including those from the eBOSS DR16 QSO and Lyman-α sample, our measurement has raised the significance level of a non-zero ΩΛ to ∼11σ. The data product of this work is publicly available at https://github.com/icosmology/eBOSS_DR16_LRGxELG and https://www.sdss.org/science/final-bao-and-rsd-measurements/.


1982 ◽  
Vol 120 ◽  
pp. 155-183 ◽  
Author(s):  
Jon Lee

We have investigated a sequence of dynamical systems corresponding to spherical truncations of the incompressible three-dimensional Navier-Stokes equations in Fourier space. For lower-order truncated systems up to the spherical truncation of wavenumber radius 4, it is concluded that the inviscid Navier-Stokes system will develop mixing (and a fortiori ergodicity) on the constant energy-helicity surface, and also isotropy of the covariance spectral tensor. This conclusion is, however, drawn not directly from the mixing definition but from the observation that one cannot evolve the trajectory numerically much beyond several characteristic corre- lation times of the smallest eddy owing to the accumulation of round-off errors. The limited evolution time is a manifestation of trajectory instability (exponential orbit separation) which underlies not only mixing, but also the stronger dynamical charac- terization of positive Kolmogorov entropy (K-system).


2015 ◽  
Vol 453 (1) ◽  
pp. 456-468 ◽  
Author(s):  
A. Burden ◽  
W. J. Percival ◽  
C. Howlett
Keyword(s):  

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