scholarly journals Dense Label Encoding for Boundary Discontinuity Free Rotation Detection

Author(s):  
Xue Yang ◽  
Liping Hou ◽  
Yue Zhou ◽  
Wentao Wang ◽  
Junchi Yan
Author(s):  
Joseph E. Mazurkiewicz

Immunocytochemistry is a powerful investigative approach in which one of the most exacting examples of specificity, that of the reaction of an antibody with its antigen, isused to localize tissue and cell specific molecules in situ. Following the introduction of fluorescent labeled antibodies in T950, a large number of molecules of biological interest had been studied with light microscopy, especially antigens involved in the pathogenesis of some diseases. However, with advances in electron microscopy, newer methods were needed which could reveal these reactions at the ultrastructural level. An electron dense label that could be coupled to an antibody without the loss of immunologic activity was desired.


Structures ◽  
2021 ◽  
Vol 32 ◽  
pp. 1320-1330
Author(s):  
Suiwen Wu ◽  
Junfei Huang ◽  
Weifeng Li ◽  
Chiyu Jiao ◽  
Jianzhong Li
Keyword(s):  

2012 ◽  
Vol 28 (3) ◽  
pp. 728-733 ◽  
Author(s):  
Giulia Piovan ◽  
Francesco Bullo
Keyword(s):  

1976 ◽  
Vol 33 (2) ◽  
pp. 789-792 ◽  
Author(s):  
S. Suzuki ◽  
M. Takagi
Keyword(s):  

2011 ◽  
Vol 2011 ◽  
pp. 1-14 ◽  
Author(s):  
Victor A. Sipachev

Structural studies are largely performed without taking into account vibrational effects or with incorrectly taking them into account. The paper presents a first-order perturbation theory analysis of the problem. It is shown that vibrational effects introduce errors on the order of 0.02 Å or larger (sometimes, up to 0.1-0.2 Å) into the results of diffraction measurements. Methods for calculating the mean rotational constants, mean-square vibrational amplitudes, vibrational corrections to internuclear distances, and asymmetry parameters are described. Problems related to low-frequency motions, including torsional motions that transform into free rotation at low excitation levels, are discussed. The algorithms described are implemented in the program available from the author (free).


1991 ◽  
Vol 27 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Malcolm B. Huglin ◽  
Jose M. Rego
Keyword(s):  

1979 ◽  
Vol 57 (22) ◽  
pp. 2896-2901 ◽  
Author(s):  
Robert A. McClelland ◽  
William F. Reynolds

Rate constants have been obtained for the acid-catalyzed N–H exchange of N-methyl, 2,N-dimethyl, and 2,4,6,N-tetramethylbenzamide and the acid-catalyzed isomerization of the three corresponding N,N-dimethylbenzamides. The ratio [Formula: see text] increases significantly with increased number of ortho methyl substituents. This is explained in terms of a suggestion of Perrin, that C—N bond rotation is not completely free in the N-protonated amide, since it must compete with a diffusion limited deprotonation reaction. The isomerization reaction, which requires such a rotation, is therefore slowed by ortho methyl substituents which hinder rotation, relative to the exchange reaction, which does not require rotation.


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