The partial discharge direction of arrival (DOA) estimation based on ultrasonic array sensor is the basic of partial discharge’s accurate positioning. The precision and searching success rate of traditional MUSIC direction finding algorithm is low. So this paper presented a new method based on ultrasonic array sensor and genetic MUSIC algorithm in partial discharge DOA estimation. The genetic MUSIC algorithm constructed fitness function by spatial spectrum estimation formula, searched the spectral peak of azimuth based on the idea of genetic iterative searching in order to obtain the global optimal solution. This paper carried out a large number of simulation experiments through the establishment of signal model and array model, then used three indicators of searching precision, searching success rate and searching time as the basis, to compare the direction finding results of classical MUSIC and genetic MUSIC. The results show that the optimization precision and searching success rate based on the genetic MUSIC algorithm is improved greatly, can obtain higher accuracy of partial discharge DOA angle.