Effect of LED lights, plant growth regulator and photoperiod on shoot production of in vitro propagated Rosa spp.

AbstractThis study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1/4MS, but was not affected by the addition of 0.1 mg/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free ¼MS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.

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Improvement of Medium Components for in vitro Cuttings of Tea Plant. (2) Optimum Concentration of Plant Growth Regulator.

Chagyo Kenkyu Hokoku (Tea Research Journal) ◽

10.5979/cha.1993.47 ◽

1993 ◽

pp. 47-55 ◽

Cited By ~ 1

Author(s):

Yukikazu KURANUKI ◽

Mariko SHIBATA

Keyword(s):

Plant Growth ◽

Growth Regulator ◽

Plant Growth Regulator ◽

Tea Plant ◽

Optimum Concentration ◽

Medium Components

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USE OF FORCING SOLUTIONS TO STUDY PLANT GROWTH REGULATOR EFFECTS ON VANHOUTTE'S SPIRAEA CULTURED IN VITRO

HortScience ◽

10.21273/hortsci.25.9.1124e ◽

1990 ◽

Vol 25 (9) ◽

pp. 1124e-1124

Author(s):

Guochen Yang ◽

P. E. Read

Keyword(s):

Plant Physiology ◽

Plant Growth ◽

Growth Regulators ◽

Growth Regulator ◽

Plant Growth Regulator ◽

Woody Plant ◽

Shoot Proliferation ◽

Plant Tissues ◽

Forcing Solution

Vanhoutte's spiraea has been propagated in vitro using explants from softwood growth of dormant stems forced in a solution containing 200 mg/l 8-hydroxyquinoline citrate (8-HQC) and 2% sucrose (Yang and Read, 1989). Objectives to further utilize this system were to determine the feasibility of applying plant growth regulators (PGR) via the forcing solution to softwood growth from forced dormant stems and to study the resulting influence on in vitro culture. BA and GA3 were placed in the forcing solution at various concentrations, including a zero PGR control. Explants were cultured on Linsmaier and Skoog (LS) medium containing zero PGR or different amounts of BA or thidiazuron (TDZ) or combinations of BA and IAA. Control explants placed on LS medium supplemented with 5uM BA with or without 1 or 5uM IAA, or with 0.5 or 0.75 uM TDZ alone produced the best shoot proliferation. BA in the forcing solution stimulated micropropagation, while GA3 caused less proliferation than explants from control solutions. Forcing solutions containing PGR are useful for manipulating responses of plant tissues cultured in vitro and for studying PGR influence on woody plant physiology.

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