Vietnam Journal of Biotechnology
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Published By "Publishing House For Science And Technology Vietnam, Vietnam Academy Of Science And Technology"

1811-4989, 1811-4989
Updated Saturday, 16 October 2021

2021 ◽  
Vol 19 (3) ◽  
pp. 547-555
Author(s):  
Le Thi Anh Hong ◽  
Pham Thi Minh Ngoc ◽  
Duong Khanh ◽  
Vo Van Tuan ◽  
Nguyen Hoang Dung

Streptococcus agalactiae is one of the microbial pathogens causing the dark-body disease on snakeskin gourami fish (Trichogaster pectoralis) that affects the growth and quality of fish. This research aimed to isolate and select bacteria inhibiting S. agalactiae which are able to use for controling pathogenic bacteria instead of antibiotics. Fourteen bacteria strains were isolated and screened from healthy fishes, sediment and water samples at fish ponds in Dong Thap province. Among these strains, L7 strain showed the highest inhibition ability with the clear zone diameter was 9,3 mm. The results of the 16S rRNA sequence analysis indicated that the L7 strain belonged to Bacillus subtilis. The experiment to evaluate the inhibition capacity and fish disease control of selected B. subtilis in experimental conditions was conducted by challenging fish with S. agalactiae. Fishes in the control treatment was infected with S. agalactiae at 106 CFU/mL had survival rate 41,7%. The experimental treatments NT1, NT2, NT3 which were treated with B. subtilis at concentrations of 105 CFU/mL, 106 CFU/mL, and 107 CFU/mL gave higher survival rates compared with the non-treated control, with the rates of 60%, 76,7%, and 81,7%, respectively. These results revealed that the isolated B. subtilis is potential used in control dark-body disease on snakeskin gourami fish.


2021 ◽  
Vol 19 (3) ◽  
pp. 495-507
Author(s):  
Huynh Thi Luy ◽  
Nguyen Huu Ho ◽  
Bui Van Le

Schefflera octophylla (Lour.) Harms is a precious plant species belonging to the Araliaceae family. All parts of plant have been used to create products for human health. In tissue culture of medicinal plants, the induction and multiplication of adventitious root of Schefflera octophylla for biomass collection have been studied. In this report, results on induction of adventitious root from leaf explants cultured in vitro of this plant species were presented. Leaf disks (~ 10 x 10 mm), leaf transverse - thin cell layers (t-TCLs) (~ 3 x 10 mm) were cultured on different mineral media MS, ½MS, B5, SH with NAA (0 - 5 mg/L), sucrose (0 - 50 g/L) and light intensity (0 - 4,000 lux). The results showed that, 30 days after culturing on ½MS solid medium plus 3 mg/L NAA, and 30 g/L sucrose in 4,000 lux light condition, direct formation of adventitious root was best from leaf disks, t-TCLs with rooting rate (%) 100, 100; root number/sample 68.80, 21.96; root lenght (mm) 16.53, 15.53, respectively. Leaf disk culture resulted in better rooting than t-TCL culture in two criteria of root number and root length. Morphological and histological observations of adventitious root primordia formation in the leaf disk were also performed. This is the first report on direct formation of adventitious root by in vitro culture of leaf disks/t-TCLs in Schefflera octophylla with very high efficiency, creating basis for further studies on root biomass multiplication for production of bioactive compounds.  


2021 ◽  
Vol 19 (3) ◽  
pp. 509-517
Author(s):  
Nguyen Khanh Hoang Viet ◽  
Ha Thi Thuy Hoa ◽  
Truong Nam Hai ◽  
Do Thi Huyen

A gene coding for GH5 endoglucanase exploited from metagnomic DNA data of bacteria in Vietnamese goats’ rumen was modularity structure including a catalytic module, a fibronectin-3 like module and an X module. The recombinant enzyme was sucessfully expressed in E. coli and purified.  To study the effect of some metal ions and chemicals on enzyme activity, in this study, we used some tools including Swiss-Prot, ProFunc, COFACTOR for prediction of enzyme structure and ligands interaction. The obtained results indicated that the most similar structure with enzyme had two conserved residues (Asp-190 và Asp-192) linked with Mn2+ within a radius of ~ 3.5 Å from the center of ion Mn2+ and enzyme molecule contained a disulphide bond. Experimental results for essessment of the effect of some metal ions (Ca2 +, Mn2 +, Mg2+, Ni2+, K+, Co2+, Cu2+, Zn2+, Fe3+) at the final concentration of 10 mM and of six common chemicals including SDS (1%), urea (1 µM), 2-mercaptoethanol (1 µM), EDTA (1 µM), tween 80 (1mM), triton X-100 (1 µM) showed that only Mn2+ increased enzyme activity slightly at concentration of 10 mM and two times at the concentration of 40 mM Mn2+. The Mn2+ has been identified as a specific binding agent may increase the stability and activity of endoglucanase GH5.


2021 ◽  
Vol 19 (3) ◽  
pp. 529-537
Author(s):  
Le Thi Lan Anh ◽  
Vu Thi Thuong ◽  
Pham Thi Ha Giang ◽  
Bui Thi Thanh Nga ◽  
Minh Thi Hang ◽  
...  

Leptospirosis is a common zoonotic disease in the tropics and subtropics. Leptospira infected human without prompt detection and treatment will face serious consequences such as acute hepatitis-kidneys, pulmonary hemorrhage which can lead to death. Besides the MAT gold standard method, Leptosipra antigens developed by DNA recombination technology have been widely studying and applying in diagnosis of Leptospira infection in human and animal. Overcoming the disadvantages of MAT and ELISA such as complicated protocol, which requires highly qualified staff and specialized equipment, the latex agglutination method has been studied and widely used in detecting pathogens such as Salmonella, E. coli, Leptospira in the world. The advantages of this method are simple operation, fast and cheap. In the previous article, we expressed Leptospira LigB antigen in E. coli cells and successfully purified it by affinity chromatography with 98% purity. In this paper, we present the process for establishment of a Lepto-LAT kit to detect Leptospira infection in dogs. This kit had the sensitivity and specificity of 91.75% and 91.57%, respectively.


2021 ◽  
Vol 19 (3) ◽  
pp. 539-545
Author(s):  
Vo Thi Thuong Lan ◽  
Le Thi Thanh

DNA marker is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare recombinant plasmids pSY-60 which was partially digested by one restriction enzyme for generating DNA markers of 7 fragments from 60 to 420 bp. The procedure included a synthesis of 60 bp DNA fragment with EcoRI sites at both ends using PCR extension, self-ligation of the 60 bp fragments and subcloning the ligated product into plasmid, generating recombinant pSY-60. Once being cloned, 500 ng of 420 bp fragment purified from 100 µL PCR product was incompletely digested by EcoRI, sufficiently producing to 50 acrylamide gels. Our procedure for production of DNA markers could be simple, time saving and inexpensive in comparison with current ones widely used in most laboratories.


2021 ◽  
Vol 19 (3) ◽  
pp. 557-568
Author(s):  
Vu Thi Quyen ◽  
Vu Thi Thu Huyen ◽  
Nguyen Mai Anh ◽  
Nguyen Hai Dang ◽  
Doan Thi Mai Huong ◽  
...  

Actinomycetes has been extensively studied due to its ability to produce secondary compounds with high application value. Especially their antibiotic ability, more than 40% of antibiotics are derived from actinomycetes of which genus Streptomyces predominates. Moreover, marine actinomycetes have been being of great interest in recent years due to their ability to produce bioactive substances capable of providing diverse and novel chemical structures. In this study, from 8 samples of marine sediments collected in Vung Ang bay in Ha Tinh provinces, we have isolated 20 strains of actinomycetes. The strains were fermented in A1+ medium, the fermentation fluid was extracted 5 times with ethyl acetate, recovered sediment and determined antibacterial and cytotoxic activity. From the screening results, two strains with the highest antibacterial activity and highest cytotoxicity were selected ie., HT03 and HT06. Both strains had antagonistic activity of Enterococcus faecalis ATCC29212 with MICHT03= 32 μg/mL, MICHT06= 16μg/mL, with Stapphylococus aureus ATCC25923 with MICHT03= 64 μg/mL, MICHT06= 32 μg/mL and with Bacillus cereus ATCC 13245 has the same MIC = 16 μg / mL. In addition, the two strains HT03 and HT06 were able to strongly inhibit the yeast Candida albicans ATCC10231 with MICHT03= 16 μg/mL, MICHT06= 8 μg/mL. Especially, two strains, HT03 and HT06, exhibited very good toxicity on all 5 cancer cell lines (MCF-7 breast cancer cell; MDA-MB-231 breast cancer cell; lung cancer cell). NCI-H1975; HeLa cervical cancer cell; AGS gastric cancer cell) at both test concentrations of 30 µg/mL and 100µg/mL. By the analysis of 16S rRNA sequence, the results showed that the HT03 strain had the highest similarity (99.93%) to that of Streptomyces fradiaes and Streptomyces fradiae ATCC. The HT06 strain was defined to belong to Nocardiopsis synnemataformans with the 16S sequence identity of 99.89% to the Japanese standard Nocardiopsis synnemataformans DSM 44143 strain NBRC-102581.


2021 ◽  
Vol 19 (3) ◽  
pp. 411-432
Author(s):  
Do Manh Hung ◽  
Nguyen Hai Ha ◽  
Nguyen Dang Ton

      Parkinson disease (PD) is the second-most common and complex neurodegenerative disorders in humans, characterized by motor symptoms such as tremor, rigidity, bradykinesia, and non-motor symptoms such as insomnia, constipation, anxiety, depression and fatigue. Up to now, the diagnosis of PD has been mainly based on clinical symptoms with motor features being the mainstay and this limits the possibility of early detection. PD is usually diagnosis after the sixth decade of life, however about 5–10% of patients who develop the disease before the age of 50 are early-onset PD. The rapid development of genetic studies and their application may induce the early diagnosis of PD in the near future, especially for the early-onset PD. A few mechanisms have been implicated in PD pathogenesis, with α-synuclein aggregation central to the development of the disease. Multiple other processes are thought to be involved, with several studies suggesting that abnormal protein clearance, mitochondrial dysfunction, and neuroinflammation play a role in the onset and progression of PD. There are many PD patients in Vietnam, however, the studies are mainly based on clinical symtom descriptions. Given the aging of the population, the prevalence of PD is to increase dramatically, which would lead to increased urgency for the need to identify improved methods in diagnosis and treatment this disease.


2021 ◽  
Vol 19 (3) ◽  
pp. 449-457
Author(s):  
Nguyen Thi Giang An ◽  
Nguyen Quang Trung

Thyroid cancer accounts for about 1% of all types of cancers, with about 80% of cases being differentiated and 20% undifferentiated. Our study was conducted on 209 Differentiated Thyroid Carcinoma (DTC) patients treated at the Department of Nuclear Medicine of Nghe An Cancer Hospital with 91.39% of patients having papillary thyroid carcinoma, with the mean age of 47.91 ± 12.08. The geographic distribution of these patients was determined to be 68.42% coastal plains. Patients living in mountainous areas accounted for only 6.22%. For our experiment, treatment for patients including having the tumor removed and thyroid tissue destroyed using 131I. Patients were then prescribed with alternative hormones varying at different doses depending on the degree and stage of cancer. Among 209 patients, 8 patients were treated with 30mCi dose(3.83%); 196 patients treated with the dose of 100–150 mCi (93.78%) and 5 patients treated 200–250 mCi (2.39%). After 3–5 days of treatment, we observed significant changes in blood count with p<0.05. Initially, erythrocytes and hemoglobin count decreased slightly for 30–150 mCi dose patients but with 200–250 mCi dose patients, those indexes fell below the reference level. The platelet index decreased significantly after 3–5 days of treatment (p<0.05). Total leukocytes, lymphocytes and mono count decreased sharply after treatment (p<0.05) but neutrophils changed very little. After 3–6 months of treatment, the blood cell formula gradually stabilized, the recovery of red blood cells took place faster than the white blood cells and platelets. The recovery of blood cells of patients treated with doses of 200–500 mCi took place more slowly (p<0.05). Thus, the use of 131I in the treatment of DTC is a common and effective method, but they may also be at risk of impairing blood cells, especially in high-dose 131I patients. However, this change is still within the allowable limits and after 6 months of treatment, the blood cell counts tend to return to their original values. The speed of recovery depends on the individual blood cell line and the amount of treatment. This research result can be used as a reference for deciding on 131I dosage and supportive measures, interventions in treatment, helping patients avoiding the decrease of blood cells and increase the effectiveness of the treatment process.


2021 ◽  
Vol 19 (3) ◽  
pp. 471-480
Author(s):  
Nguyen Hong Chien ◽  
Nguyen Thi Kim Linh ◽  
Trin Thi Kim My ◽  
Nguyen Xuan Truong ◽  
Nguyen Van Chung ◽  
...  

Wax tree has been cultivated to get lacquer, a valuable source of material that is necessary for many industries and handicrafts. Evaluating not only phenotype but also genotype is essential in order to increase the efficiency of new breeding program. In this study, the genetic relationship at the molecular level of 90 wax trees collected in three regions, i.e., Tam Nong, Thanh Son (Phu Tho) and Chiem Hoa (Tuyen Quang), was analyzed by SSR and ScoT markers. The results revealed a significant diversity among the individuals, with similarity coefficient from 0.41 to 0.98. The sample group collected in Chiem Hoa was significantly different from that in Tam Nong. Most of samples which had a high level of genetic similarity, from 0.95 to 0.98, were pairs of samples at the same geographical origin. The high similarity degree of these samples is likely due to the practice of selecting seeds from high-yield wax tree to be kept for seeds. These results provide the necessary information for new wax tree breeding program.


2021 ◽  
Vol 19 (3) ◽  
pp. 569-575
Author(s):  
Nguyen Thi My Yen ◽  
Tran Thanh Thai ◽  
Ngo Xuan Quang ◽  
Pham Ngoc Hoai

The researchs on the biomass spectra - a functional characteristic of biotic communities is still limited. In this study, the nematode biomass spectra in the bottom of Ba Lai estuary was investigated at six subtidal stations from the sea toward the upstream. The result showed that nematode biomass spectra ranged between -8 and 1 being significantly different between stations, and the lowest biomass of those spectras was in station BL4 (< 2 µg) which is upwardly closed to the Ba Lai dam. BL4 was also characterized by the lowest nematode abundance in the studied area. In addition, station BL3 downwardly closed to the dam exhibited low number of individuals. The heterogeneity in the nematode biomass spectra of BL3 and BL4 might due to the disturbance in the sedimentary environment of Ba Lai estuary related to the dam impact. This research again supports the important role of biomass spectra as bioindicator tool for biomonitoring and environmental quality assessment. Therefore, applying nematode biomass spectra is recommended for environmental assessment due to their advantages such as timesaving, not taxonomical expertise-requirement.


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