There were executed studies to assess the impact of broadband pulsed electromagnetic radiation on the central nervous system of white mice in test “open field”, “T-maze”, “Forced swimming”. The results of the study proved the influence of pulsed electromagnetic radiation on cognitive function. Experimental studies were performed in a shielded equipped semi-anechoic chambers with the remote control of the work of the simulator of broadband pulse EMT. At the end of the performed studies it was revealed that when exposed to broadband pulsed electromagnetic radiation into three groups of animals (exposure of 15, 30, 60 minutes) only in the group with exposure for 15 minutes there were no detected changes in behavioral responses. In the other two groups there were shown statistically significant changes in indices of the horizontal and vertical locomotor activity. The results confirm the possibility of separation between the processes of inhibition and excitation under the influence of EMF of low intensity (non-thermal) on biological objects.