A 3-D deconvolution based particle detection method for wide-field microscopy image

Author(s):  
Jing Zhao ◽  
Yang Li ◽  
Sidan Du
2012 ◽  
Author(s):  
James Dou ◽  
Lu Chen ◽  
Rakesh Nayyar ◽  
Stewart Aitchison

2014 ◽  
Vol 945-949 ◽  
pp. 1558-1560
Author(s):  
Zhong Min Li ◽  
Li Fei Mei ◽  
Mao Song

Infrared weak small target detection is one of the key technologies in the early infrared imaging guidance and wide-field view surveillance system. In the complex and low signal-to-noise ratio background, the target has only a few pixels. There is no shape and texture information to use. It brings great difficulties to the infrared weak small target detection. In this paper, we sum up the research status of infrared weak small target detection method, and analyze the key problems of infrared weak small targets detection.


Author(s):  
Peter Shaw ◽  
Alison Beven ◽  
David Rawlins ◽  
Martin Highett

Fluorescent in situ hybridization and 3-D confocal microscopy have been used to study the arrangement of genes and transcripts in interphase plant nuclei. Wide-field fluorescence microscopy coupled with computer deconvolution has also been used on the same specimens and gives comparable results to confocal imaging. When image deconvolution is applied to confocal images, these show a significant improvement. A comparison of confocal and wide-field (CCD) images of the same specimen (a plant nucleolus labelled with probe to the rRNA genes) is shown in Figure 1. The deconvoluted wide-field data (Fig 1b) and the raw confocal data (Fig 1c) are remarkably similar; the former is somewhat clearer. The deconvoluted confocal data (Fig 1d) is clearest of all.Double-stranded DNA and single-stranded RNA probes to the 45S and 5S RNA genes have been used to investigate the functional organization of the nucleolus in pea root tissue. In situ hybridization was carried out on vibratome slices approximately 50μm thick (about 3 cells).


2021 ◽  
Vol 42 (16) ◽  
pp. 6315-6332
Author(s):  
Zhipeng Dong ◽  
Yanxiong Liu ◽  
Wenxue Xu ◽  
Yikai Feng ◽  
Yilan Chen ◽  
...  

Sensors ◽  
2021 ◽  
Vol 22 (1) ◽  
pp. 202
Author(s):  
Zeqian Wu ◽  
Jinxing Cheng ◽  
Mei Xu ◽  
Qingbo Wang ◽  
Ai Yu ◽  
...  

The detection of α particles is of great significance in military and civil nuclear facility management. At present, the contact method is mainly used to detect α particles, but its shortcomings limit the broad application of this method. In recent years, preliminary research on noncontact α-particle detection methods has been carried out. In this paper, the theory of noncontact α-particles detection methods is introduced and studied. We also review the direct detection and imaging methods of α particles based on the different wavelengths of fluorescence photons, and analyze the application and development of this method, providing an important reference for researchers to carry out related work.


2008 ◽  
Vol 1 (3-4) ◽  
pp. 241-246
Author(s):  
Guoqiang Sun ◽  
Jihong Zheng ◽  
Gang Zheng ◽  
Songlin Zhuang

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