In Vivo Measurement of Brain Tissue Response After Irradiation: Comparison of T2 Relaxation, Apparent Diffusion Coefficient, and Electrical Conductivity

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Myriam M. Chaumeil ◽  
Robert Bok ◽  
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K. Krnjević

Ca2+-sensitive microelectrodes, attached to CaCl2-containing micropipettes, were inserted into the dorsal hippocampus of rats under urethane. When Ca2+ was released iontophoretically, the amplitude and time course of the resultant increase in extracellular Ca2+ concentration could be fitted to a simple diffusion model, but the apparent diffusion coefficient of Ca2+ was only about 1/100 of its value in water, possibly because of reversible Ca2+ binding to hippocampal tissue. A further anomaly was a very low transport number (<0.01) for the release of Ca2+ from microelectrodes in vivo.


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